Southeast Asian Fisheries Development Center, Aquaculture Department Institutional Repository (SAIR) is the official digital repository of scholarly and research information of the department. This is to enable the effective dissemination of AQD researchers' in-house and external publications for free and online. The repository uses DSpace, an open source software, developed at Massachusetts Institute of Technology (MIT) Libraries. It is an Open Archives Initiative (OAI)-compliant.

Initially, the repository shall contain preprints, full-texts or abstracts of journal articles, books and conference proceedings written by SEAFDEC/AQD scientists and researchers. The aim is to promote these publications especially those published in international peer-reviewed journals and generate higher citation through increased visibility.

It will also provide free access to all in-house publications of SEAFDEC/AQD. Full-text digitized copies of fishfarmer-friendly materials like books, handbooks, policy guidebooks, conference proceedings, extension manuals, institutional reports, annual reports (AQD Highlights), and newsletters (SEAFDEC Asian Aquaculture, Aqua Farm News, AquaDept News and AQD Matters) can be retrieved and downloaded.

In the future, SAIR will expand its collection to include images, presentations, audios, and videos among others.

The objectives of the repository are to: (1) to provide reliable means for SEAFDEC/AQD researchers to store, preserve and share their research outputs and (2) to provide easy access and increase the visibility of SEAFDEC/AQD scientific publications

SAIR also aims to encourage SEAFDEC/AQD researchers for self-archiving and submitting pre-prints from which metadata will be screened and approved by the library staff.

  • Article

    Dietary soy peptide enhances thermotolerance and survival of juvenile japanese flounder, Paralichthys olivaceus 

    JA Ragaza, REP Mamauag, S Yokoyama, M Ishikawa & S Koshio - Journal of the World Aquaculture Society, 2015 - World Aquaculture Society
    Soy peptide (SP), a soy protein enzymatic hydrolysate, contains bioactive substances that could be utilized as an immune-stimulating feed ingredient. The experiment evaluated the efficacy of dietary SP on promoting growth, and enhancing tolerance and survival to heat stress in juvenile Japanese flounder, Paralichthys olivaceus. Four diets were incorporated with different levels of SP (0, 2, 5, and 10%) and a 6-wk feeding trial ensued. Following the feeding trial, the experimental groups were subjected to heat stress to measure survival rate and heat shock protein 70s (HSP70s) in gill, liver, and skin. Fish fed diets with SP inclusion showed considerable decrease in percent weight gain. Significantly higher lethal time values to 50% mortality (LT50) value were recorded for fish fed 10% SP. Moreover, LT50 values of fish fed 2 and 5% SP were significantly higher compared with fish fed control diet. HSP70s produced in all the tissues were significantly highest in fish fed 10% SP. HSP70s values were significantly higher in fish fed 2 and 5% SP compared with fish fed control diet. A significant reduction in HSP70s among all groups during recovery period was also observed. These results suggest that SP can be used to enhance the immune response and survival of P. olivaceus under heat stress.

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  • Article

    Mortality of pond-cultured Litopenaeus vannamei associated with acute hepatopancreatic necrosis disease (AHPND) and white spot syndrome virus (WSSV) infection in the Philippines 

    LD de la Peña, NAR Cabillon, EC Amar, DD Catedral, RC Usero, JP Faisan Jr., JI Arboleda, WD Monotilla, AT Calpe, DD Fernandez & CP Saloma - Fish Pathology, 2017 - Japanese Society of Fish Pathology
    Mortalities of up to 60% were observed in pond-cultured Litopenaeus vannamei in Bohol, Philippines. Histopathological examination revealed typical acute hepatopancreatic necrosis disease (AHPND) pathology. PCR test generated 1,269 bp and 230 bp amplicons confirmative for the toxin-producing AHPND strain of Vibrio parahaemolyticus among shrimp sampled from eight ponds. The same samples were subjected to PCR analyses for the presence of other viruses, namely WSSV, IHHNV, IMNV, and TSV. The samples were negative for the viruses except WSSV, which was detected after one-step PCR in six out of eight ponds. These results suggested that shrimp were infected dually with AHPND V. parahaemolyticus and WSSV.
  • Article

    A probiotic Bacillus strain containing amorphous poly-beta-hydroxybutyrate (PHB) stimulates the innate immune response of Penaeus monodon postlarvae 

    JLQ Laranja, EC Amar, GL Ludevese-Pascual, Y Niu, MJ Geaga, P De Schryver & P Bossier - Fish and Shellfish Immunology, 2017 - Elsevier
    In this study, the PHB-accumulating Bacillus sp. JL47 strain (capable of accumulating 55% PHB on cell dry weight) was investigated for its effects on the immune response of giant tiger shrimp (Penaeus monodon) postlarvae (PL) before and after the Vibrio campbellii challenge. Briefly, shrimp PL were cultured and fed with Artemia nauplii enriched with Bacillus sp. JL47. Shrimp receiving the Artemia nauplii without JL47 enrichment were used as control. After 15 days of feeding, the shrimp were challenged with pathogenic V. campbellii LMG 21363 at 106 cells mL-1 by immersion. Relative expression of the immune related genes encoding for prophenoloxidase (proPO), transglutaminase (TGase) and heat shock protein 70 (Hsp70) in the shrimp were measured before (0 h) and after (3, 6, 9, 12, 24 h) the Vibrio challenge by quantitative real-time PCR using β-actin as the reference gene. The expressions of TGase and proPO were significantly up-regulated (p < 0.05) within 9 h and 12 h, respectively after challenge in shrimp receiving the Bacillus sp. JL47 as compared to the challenged and non-challenged controls. Hsp70 expression was significantly increased (p < 0.05) at 3 h post-challenge in all challenged shrimp. Interestingly, proPO and TGase genes were significantly up-regulated (p < 0.05) in Bacillus sp. JL47 treated shrimp even before the Vibrio challenge was applied. No up-regulation in the Hsp70 gene, however, was observed under these conditions. The data suggest that the protective effect of the PHB-accumulating Bacillus sp. JL47 in shrimp was due to its capacity to stimulate the innate immune related genes of the shrimp, specifically the proPO and TGase genes. The application of probiotic Bacillus species, capable of accumulating a significant amount of PHB, is suggested as potential immunostimulatory strategy for aquaculture.
  • Article

    Optimum low salinity to reduce cannibalism and improve survival of the larvae of freshwater African catfish Clarias gariepinus 

    G Kawamura, T Bagarinao, ASK Yong, PW Sao, LS Lim & S Senoo - Fisheries Science, 2017 - Springer Verlag
    The freshwater African catfish Clarias gariepinus is carnivorous and cannibalistic even during the larval and juvenile stages and this behavior causes economic losses in aquaculture. This study examined for the first time the effect of salinity on cannibalism, survival, and growth of African catfish larvae in the hatchery. Larvae (4 days old, median 7.8 mm TL, 2.8 mg BW) of the African catfish were reared for 21 days at nominal salinity 0, 1, 2, 3, 4, 5, 6, and 7 ppt. After 21 days, they grew to 10–39 mm (median 22 mm) and 10–490 mg (median 90 mg), with no significant difference by salinity treatments. Survival ratios were similarly low (24–31%) at 0, 1, 3, and 7 ppt and significantly higher (49–55%) at 2, 4, 5, and 6 ppt. Cannibalism was significantly lower, 15–30% at 4–6 ppt, than the 40–50% at 0–3 and 7 ppt. Size variation was lower at 4–6 ppt and higher at 0–3 and 7 ppt. We recommend hatchery rearing of African catfish at the optimum low salinity of 4–6 ppt rather than in full fresh water at least up to 21 days. This rearing method fosters larval welfare and improves hatchery production.
  • Article

    Shelter colour preference of the postlarvae of the giant freshwater prawn Macrobrachium rosenbergii 

    G Kawamura, T Bagarinao, ASK Yong, TC Fen & LS Lim - Fisheries Science, 2017 - Springer Verlag
    The addition of artificial shelters of various materials has been used in Macrobrachium rosenbergii tanks and ponds as a means of increasing productivity. The present study investigated the shelter colour preference of M. rosenbergii postlarvae (age 15–18 days after metamorphosis) in the laboratory. Shelter occupancy tests were performed on four groups of 200 postlarvae in four 57-l aquaria, into which were placed shelters made of rigid coloured netting sewn into four-layered cubes open on two sides. The shelters were presented in six colour pairs: black vs dark green, black vs light green, black vs blue, dark green vs light green, dark green vs blue, and light green vs blue. Colour preference was tested six times for each colour pair. The data obtained were analyzed using Thurstone’s law of comparative judgment. The mean z-score was significantly highest (i.e., highest preference) for the black shelter. Vision was involved in the detection of and approach to the shelter. When released in aquaria, intact-eyed individual postlarvae directly approached the shelter straight away, whereas blind postlarvae (paint over the eyes) swam around randomly and occupied the shelter only after accidental contact much later. Postlarvae in open water out of the shelter exhibited frequent aggressive contact, while those in the shelter were quiescent. The use of black shelters in rearing tanks is thus recommended for reducing stress and aggression among M. rosenbergii postlarvae in the hatchery.
  • Article

    Clonal production of Kappaphycus alvarezii (Doty) Doty in vitro 

    MRJ Luhan & JP Mateo - Journal of Applied Phycology, 2017 - Springer Verlag
    Micropropagation has proven to be a reliable method to mass produce certain crops. This method also has been applied in macroalgae to produce clones for seaweed farming. Protocols for callus production and shoot regeneration from protoplasts have been established for some seaweed species like Kappaphycus alvarezii. Cells and larger tissues, whether in solid or suspension medium, have been used to propagate clones which were later tested for suitability for farming. Although clonal production was successful, the long duration of culture in vitro limits the production process making the growing of Kappaphycus in vitro an expensive technique to produce clones. In this study, K. alvarezii was grown in vitro to develop a more efficient protocol for the production of clones. Small sections of Kappaphycus were grown in suspension for 1 month under the same temperature, light, and salinity. The type of media, source of explants, length of explants, and stocking density that resulted in the highest growth rate and survival rate were determined. Growth rate of K. alvarezii is significantly higher in media with inorganic nitrogen added than in Grund medium or Ascophyllum nodosum medium only. The appearance of shoot primordia as early as 5 days was observed in media with higher nitrogen concentration. Growth rates of explants approximately 3 and 5 mm are significantly higher than 10 mm sections. Shoots develop significantly faster in explants from tips than sections from older branches. Growth rate of K. alvarezii grown at 0.5, 0.75, 1, 1.25 s 10 mL−1 of medium is not significantly different. This protocol could significantly reduce the (1) time of culture and (2) cost of plantlets production by not using plant growth regulators and formulated media in vitro. Nursery reared plantlets/propagules for farming would be affordable to the stakeholders for sustainability of seaweed production.

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