Southeast Asian Fisheries Development Center, Aquaculture Department Institutional Repository (SAIR) is the official digital repository of scholarly and research information of the department. This is to enable the effective dissemination of AQD researchers' in-house and external publications for free and online. The repository uses DSpace, an open source software, developed at Massachusetts Institute of Technology (MIT) Libraries. It is an Open Archives Initiative (OAI)-compliant.

Initially, the repository shall contain preprints, full-texts or abstracts of journal articles, books and conference proceedings written by SEAFDEC/AQD scientists and researchers. The aim is to promote these publications especially those published in international peer-reviewed journals and generate higher citation through increased visibility.

It will also provide free and open access to all in-house publications of SEAFDEC/AQD. Full-text digitized copies of fishfarmer-friendly materials like books, handbooks, policy guidebooks, conference proceedings, extension manuals, institutional reports, annual reports (AQD Highlights), and newsletters (SEAFDEC Asian Aquaculture, Aqua Farm News, AquaDept News and AQD Matters) can be retrieved and downloaded.

In the future, SAIR will expand its collection to include images, presentations, audios, and videos among others.

The objectives of the repository are to: (1) to provide reliable means for SEAFDEC/AQD researchers to store, preserve and share their research outputs and (2) to provide easy access and increase the visibility of SEAFDEC/AQD scientific publications

SAIR also aims to encourage SEAFDEC/AQD researchers for self-archiving and submitting pre-prints from which metadata will be screened and approved by the library staff.

  • Article

    Development and characterization of nine novel microsatellite markers for the milkfish Chanos chanos 

    BS Santos, MRR Romana-Eguia, ZU Basiao & M Ikeda - Conservation Genetics Resources, 2015 - Springer Verlag
    The milkfish, Chanos chanos, is an important aquaculture resource in Southeast Asia. Using NGS data, 72 microsatellite markers were developed. PCR product confirmation using agarose gel electrophoresis allowed the identification of 24 utilizable markers. Milkfish samples (n = 48) from a hatchery stock in Palawan, Philippines were analyzed for genetic variability at the aforementioned 24 loci. Consequently, nine of these microsatellite loci were noted to have high success rate in amplification, exhibited polymorphism with 19 maximum number of alleles and no null alleles. These are the first microsatellite markers to be developed and characterized for C. chanos that will efficiently enable genetic stock delineation and monitoring as well as marker-aided genetic improvement research.

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  • Article

    Immunization regimen in Asian sea bass (Lates calcarifer) broodfish: A practical strategy to control vertical transmission of nervous necrosis virus during seed production 

    R Pakingking Jr., EG de Jesus-Ayson, O Reyes & NB Bautista - Vaccine, 2018 - Elsevier
    Outbreaks of viral nervous necrosis (VNN) in Asian sea bass (Lates calcarifer) at the larval stages via vertical transmission of nervous necrosis virus (NNV) from asymptomatic broodfish remain as a major deterrent during seed production. A five-year study was conducted to produce NNV-specific-free sea bass broodfish reared in land-based tanks through an annual immunization regimen with the formalin-inactivated NNV. We primarily immunized (intraperitoneal injection) sea bass juveniles (5 g) and monitored the neutralizing antibody (Nab) titers in the sera of these fish at scheduled intervals post-immunization. Nab titers in the sera of immunized fish peaked at Month 2 (titer: 1:4480 ± 1185) but thereafter gradually declined and significantly dropped (1:260 ± 83) at Month 12 post-primary immunization. Booster immunization of these fish at Month 12 post-immunization led to abrupt increases in Nab titers in booster immunized (B-Im) fish at Month 1 (1:12800 ± 6704) but thereafter declined and dropped at Month 12 (1:480 ± 165) post-booster immunization. The annual booster injections with the inactivated vaccine or L-15 (Unimmunized [U-Im]) were consecutively conducted for 4 years until the fish became sexually mature. Mature fish from both groups were successively induced to spawn twice (1-month interval) via intramuscular injection with luteinizing hormone-releasing hormone analogue (LHRH-a; 100 µg/kg BW). NNV was not detected by RT-PCR in oocytes and milts, and spawned eggs of B-Im fish. In contrast, oocytes and milts, and spawned eggs of U-Im fish were NNV positive. Spawned eggs of B-Im broodfish exhibited Nab titers ranging from 1:192 ± 34 to 1:240 while such was not detected (<1:40) in eggs of U-Im fish. Taken together, current data clearly demonstrate that annual immunization regimen with inactivated NNV vaccine is a pragmatic approach for sustaining immunocompetent sea bass broodfish reared in land-based tanks and circumvent the risk of vertical transmission of NNV from asymptomatic broodfish to their offspring under stress of repetitive spawning.
  • Article

    Responsible aquaculturists: The information-seeking behavior of milkfish farmers in Iloilo, Philippines 

    DL Superio, ES Nemiz, MGH Oliveros, VEV Pacullo, AMA Yap-Zerrudo & JB Canaman - Information Development, 2018 - SAGE Publications
    Compliance with a policy, law, standard or code requires understanding of its provisions. However, for someone to understand it, he must be aware of its existence and be provided access to it. A qualitative-quantitative research was conducted to determine the awareness of milkfish farmers about the Philippine Code of Practice for Aquaculture in the municipalities of Leganes and Zarraga, Iloilo Province, the Philippines and their information-seeking behaviors. Results revealed that the majority of the respondents were not aware of the existence of the Code, hence, there is a low level of compliance. When seeking everyday life information, the majority of the milkfish farmers depended on television, personal or person-to-person communication and radio, while when seeking for fish farming information, personal communication was the preferred source. None of the respondents was aware of the existence of their municipal libraries.
  • Article

    Status of sea cucumber fishery and populations across sites with different levels of management in Palawan, Philippines 

    JBS Jontila, HM Monteclaro, GF Quinitio, SM Santander-de Leon & JP Altamirano - Ocean and Coastal Management, 2018 - Elsevier
    This study was conducted to investigate the sea cucumber fishery and compare populations between exploited and unexploited sites. Three islands were selected, the Arrecife Island as the unexploited site and the Johnson and Green Islands representing the exploited sites. In each site, sea cucumber species richness, diversity and densities were assessed by laying as much as 15 transects (50 m × 5 m) per habitat in each island. Differences in species richness, diversity and relative densities across sites and habitats were tested using a Two-way Analysis of Variance (ANOVA) and Tukey test for post hoc comparisons. An interview with gatherers, key informants and focus group discussions were also conducted to gather information on the sea cucumber fishery and its management. Results showed that the unexploited site had a higher species richness (14 vs. 7 and 7). Relative density of species was also higher in the unexploited site at 1245 ind ha-1 while it was only 78 and 39 ind ha-1 in exploited sites (p < 0.05). Interviews and a focus group discussion revealed that national regulations on permit system and size limits were not enforced at the local level and that gatherers had low levels of awareness regarding regulations on sea cucumber harvest. Current management measures appear to be ineffective and insufficient, leaving the sea cucumbers in areas open to exploitation at risk of depletion. Given that sea cucumbers provide substantial income to local communities, the depletion of this resource could compromise the livelihood of people in island communities that rely heavily on marine resources for living. Thus, this study highlights the need to review national policies on sea cucumber fishery and place the management at the local level.
  • Article

    Quality assessment of newly hatched mud crab, Scylla serrata, larvae 

    ET Quinitio, JJ dela Cruz-Huervana & FD Parado-Estepa - Aquaculture Research, 2018 - Wiley
    Starvation and exposure to formalin were investigated as possible stress tests for evaluating the quality of mud crab, Scylla serrata, larvae. For the starvation stress test, newly hatched zoeae stocked in 150-ml containers were either starved or fed rotifers. Similarly, newly hatched zoeae were stocked in containers with seawater of 0 (control), 20, 30 and 40 mg/L formalin for the formalin stress test. The zoeae from the same batches were used for seed production to monitor their performance and validate the results of stress tests. Starvation was found to be unsuitable for larval quality evaluation. However, the impact of initial food deprivation on the newly hatched larvae indicates that feeding immediately after hatching is necessary for mud crab larvae. Exposure of larvae to 40 mg/L formalin for 3 hr appeared to be a reliable and practical method for larval quality assessment as the survival of larvae in the mass production tanks validated the classification of good and poor quality batches in the stress tests. On this basis, a hatchery operator can decide which batch should be cultured further. Finally, there appears to be a link between the quality of larvae and the performance at the megalopa and early juvenile crabs.
  • Newsletter

    AQD Matters 2018 March - April 

    JMV de la Cruz (Ed.) - 2018 - Aquaculture Department, Southeast Asian Fisheries Development Center

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