02 SEAFDEC/AQD Collaborative Publications
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Differentiation of crocodilian granulocytes using histochemical techniques
(Fish Health Section, Asian Fisheries Society, 2002)Blood smears from 54 crocodiles of three breeds (Crocodylus siamensis, C. porosus, and crossbred) were examined after Wright-Giemsa staining. Detection for alkaline phosphatase and peroxidase activities were performed. ... -
Isolation and characterization of an Achlya sp. associated with diseased soft-shelled turtle (Trionyx sinensis) from Hong Kong
(Fish Health Section, Asian Fisheries Society, 2002)In September 1996, a disease outbreak was reported in one turtle farm in the New Territories, Hong Kong. Soft-shelled turtle (Trionyx sinensis) cultured in tanks showed white spots on their body surface and carapace resulting ... -
Bacterial diseases of cultured tiger frog (Rana tigerina)
(Fish Health Section, Asian Fisheries Society, 2002)Diseased cultured tiger frogs (Rana tigerina), from 173 farms throughout Thailand, were submitted for diagnosis at Aquatic Animal Health Research Institute from 1994-1998. Bacterial species were isolated from sick frogs ... -
Isolation of FV3-like iridovirus from a cutaneous ulceration disease of cultured frog, Rana tigrina Cantor, in Thailand
(Fish Health Section, Asian Fisheries Society, 2002)A new frog disease occurred in frog culture farms located in Central Thailand since early 1998. The disease affects 20-100% of the frog population in affected farms. Diseased adult frogs usually exhibit ulcerative lesions ... -
Hematological changes in snakeskin gourami, Trichogaster pectoralis, affected by epizootic ulcerative syndrome
(Fish Health Section, Asian Fisheries Society, 2002)Selected hematological parameters of the circulating blood of snakeskin gourami, Trichogaster pectoralis, naturally affected by epizootic ulcerative syndrome (EUS) were compared with those of apparently healthy fish collected ... -
Cross-sectional survey of epizootic ulcerative syndrome (EUS) cases in Bangladesh
(Fish Health Section, Asian Fisheries Society, 2002)An interview-based questionnaire survey of one fish farmer and one fisherman randomly selected in each of the 64 districts of Bangladesh was carried out to study prevalence of EUS, and risk factors associated with EUS ... -
Epizootic ulcerative syndrome of fishes: rhabdovirus infection and EUS induction experiments in snakehead fish
(Fish Health Section, Asian Fisheries Society, 2002)Rhabdoviral agents have long been found to be associated with Epizootic Ulcerative Syndrome (EUS) although the presence of granulomas caused by the fungus Aphanomyces invadans is currently used as the diagnostic criterium ... -
VP2 protein carries neutralization epitopes in blotched snakehead virus (BSNV)
(Fish Health Section, Asian Fisheries Society, 2002)A new aquabirnavirus (BSNV, blotched snakehead virus) was isolated from a persistently infected warmwater fish cell line (BSN) developed from blotched snakehead (Channa lucius). Four polypeptides of molecular mass 112 ... -
Genome analysis of Pasteurella piscicida
(Fish Health Section, Asian Fisheries Society, 2002)Genome analysis was conducted to detect the virulence genes in Pasteurella piscicida (Photobacterium damsela subsp. piscicida) KP9038. To date, we have analyzed 366 clones and determined 333,341 bp nucleotide sequences. ... -
Cloning of biodefense and immune-related genes of Japanese flounder, Paralichthys olivaceus
(Fish Health Section, Asian Fisheries Society, 2002)A Japanese flounder Paralichthys olivaceus cDNA library was constructed from leukocytes that had been previously infected with hirame rhabdovirus (HRV). Eight hundred ninety six independent clones were isolated from which ... -
Production and characterization of a monoclonal antibody against Aeromonas hydrophila
(Fish Health Section, Asian Fisheries Society, 2002)A monoclonal antibody (MAb), 8D10, was produced against Aeromonas hydrophila strain 94030 isolated from giant gourami (Osphronemus gourami). This was classified as an IgG2a immunoglobulin by ELISA, and recognised a 41 kDa ... -
Study of bacterial loading in macrophage cells of hybrid catfish
(Fish Health Section, Asian Fisheries Society, 2002)Hybrid catfish (Clarias macrocephalus x Clarias gariepinus) farming has developed very successfully in Thailand over the last 10 years. Farmers, however, are still encountering disease problems and, as a result, face ... -
Humoral immune response of channel catfish to Flavobacterium columnare antigens, lipopolysaccharide and formalin-killed cells, and the protective ability of these antigens
(Fish Health Section, Asian Fisheries Society, 2002)The objective of the experiment was to determine the immunogenicity of various concentrations of formalin-killed whole cells of Flavobacterium columnare and lipopolysaccharide (LPS) from the bacterium, with and without ... -
Vibriosis vaccine development: Immunological characterization of Vibrio alginolyticus
(Fish Health Section, Asian Fisheries Society, 2002)The lipopolysaccharide (LPS) profiles of five Vibrio alginolyticus strains (14, 17, 26, 78 and ATCC 17749) were studied by SDS-PAGE. Strains (ST) 14, 17 and 26 were found to possess high molecular weight bands, suggesting ... -
Finfish immunology and its use in preventing infectious diseases in cultured finfish
(Fish Health Section, Asian Fisheries Society, 2002)This review draws heavily on knowledge and experience gained from studies on cultured finfish, particularly salmonids. The advantages of using the finfish immune system as a means of protecting them against infectious ... -
In situ hybridization: A detection tool for fish pathogens and its application on recent advances on whirling disease research
(Fish Health Section, Asian Fisheries Society, 2002)A non-radioactive in situ hybridization (ISH) protocol was used as a diagnostic tool for several fish pathogens including Candidatus Xenohaliotis californiensis, the etiologic agent of withering syndrome, which is a new ... -
Effects of praziquantel on metacercarial cysts of Centrocestus sp. on the gills of goldfish Carassius auratus
(Fish Health Section, Asian Fisheries Society, 2002)Effects of praziquantel (a compound for trematode and cestode control in dogs) on metacercarial cysts of Centrocestus sp. (a digenetic trematode: Family Heterophyidae) occurring on the gills of goldfish Carassius auratus ... -
Diseases of seabass, Lates calcarifer, larvae from hatcheries in Indonesia
(Fish Health Section, Asian Fisheries Society, 2002)This paper describes three diseases of seabass (Lates calcarifer) larvae: viral nervous necrosis (VNN), glugeosis microsporidian infection, and a bacterial infection observed in private hatcheries in Indonesia from 1997-1998. ... -
Life cycle of the blood fluke Sanguinicola armata Plehn, 1905 (Digenea: Sanguinicolidae), parasite of freshwater fishes in Malaysia
(Fish Health Section, Asian Fisheries Society, 2002)The blood fluke Sanguinocola armata is a parasite with a simple, two-host life cycle involving an aquatic mollusc, the intermediate host where cercariae develop, and a fish, which is the definitive host. In Malaysia, S. ... -
Recent advances in fish parasitology in Japan
(Fish Health Section, Asian Fisheries Society, 2002)Recent advances in fish parasitological studies in Japan focus mainly on myxosporean, microsporean and monogenean infections. Myxosporeans: The two-host life cycle has been elucidated for several freshwater species ...
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