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dc.contributor.authorHara, Shiro
dc.contributor.authorCanto Jr., Jose T.
dc.contributor.authorAlmendras, Jesus M. E.
dc.date.accessioned2012-08-24T09:15:56Z
dc.date.available2012-08-24T09:15:56Z
dc.date.issued1982
dc.identifier.citationHara, S., Canto Jr., J.T., & Almendras, J.M.E. (1982). A comparative study of various extenders for milkfish, Chanos chanos (Forsskal), sperm preservation. Aquaculture, 28(3-4), 339-346. doi:10.1016/0044-8486(82)90076-Xen
dc.identifier.issn0044-8486
dc.identifier.urihttp://hdl.handle.net/10862/1041
dc.description.abstractVarious extenders, containing potassium chloride, sodium chloride, glucose, sodium citrate, Ringer's solution, cow serum and milkfish serum were used to preserve milkfish (Chanos chanos) sperm at near-zero temperatures (0-4°C) and in liquid nitrogen (?196°C). Milkfish serum was a superior extender in both cases. After 5 days, comparatively good motility (> 30%) and fertilizing capacity (6.7-18.9%) were observed in the near-zero liquid samples, while in other extenders, sperm ceased to show motility after 2 days. The fertilization success of 4-5 days cryopreserved sperm averaged 67.5% (n = 2) with milkfish serum, 60.5% (n = 2) with 400 mM glucose, 58.0% (n = 2) with 150 mM sodium chloride, 41.2% (n = 1) with Ringer's solution and 31.9% (n = 2) with cow serum.en
dc.language.isoenen
dc.publisherElsevieren
dc.subjectChanos chanosen
dc.subjectSpermen
dc.subjectPreservativesen
dc.subjectFreezing storageen
dc.subjectAquaculture techniquesen
dc.subjectMilkfishen
dc.subjectFertilizationen
dc.titleA comparative study of various extenders for milkfish, Chanos chanos (Forsskal), sperm preservationen
dc.typeArticleen
dc.identifier.doi10.1016/0044-8486(82)90076-X
dc.citation.volume28
dc.citation.issue3-4
dc.citation.spage339
dc.citation.epage346
dc.citation.journalTitleAquacultureen


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