Influence of nutrition on the hepatocytes of Chanos chanos (Chanidae: Teleostei)
Abstract
The hepatocytes of milkfish (C. chanos) fry and fingerlings offered a variety of diets differ considerably as was shown by means of transmission electron microscopy. For fry it was shown that a 7 day starvation period results in a heavily altered hepatocyte ultrastructure but that even in this stadium fast regeneration was possible. Chlorella turned out to be the worst diet, among the artificial diets, the trout diet provoked the best regeneration. In fingerlings a prolonged starvation period was necessary to affect hepatocytes. In some cases considerable indications of liver cell degeneration were found after feeding certain diets for 2-3 months. Even dried lumut and lab-lab did not create optimal hepatocyte ultrastructure. Feeding with cod liver oil did not result in deposition of lipid droplets in the hepatocytes of fingerlings, what is in contrast to milkfish fry hepatocytes.
Citation
Storch, V., Segner, H., Juario, J. V., & Duray, M. N. (1984). Influence of nutrition on the hepatocytes of Chanos chanos (Chanidae: Teleostei).Publisher
ElsevierSubject
Type
ArticleISSN
0044-5231Acknowledgment/Sponsorship
This work was financially supported by DFG Sto 75/8-9, DAAD 951 322 002/3, and IDRC 3-P.740146.
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Article
Lactate dehydrogenase isozyme patterns during the development of milkfish, (Chanos chanos (Forskal))
-Kalikasan, The Philippine Journal of Biology , 1981 - University of the Philippines at Los BañosPolyacrylamide disc gel electrophoresis was done to determine the lactate dehydrogenase (LDH) isozyme patterns for fry (5-3 mg), fingerling (6-12 g), pond-size (150-250 g) and adult (6-9 kg) milkfish. The patterns were tissue specific; the different tissues examined, viz., eye, liver, heart, and skeletal muscle had different expressions of LDH isozymes. The resolved patterns appeared to be products of LDH gene loci A, B, and C. Subunits A and B were present in all tissues. A4 and B4 were predominant in skeletal and heart muscle, respectively; the two associated non-randomly in vivo and formed only the heteropolymers A3B and AB3. A liver band, L4, was most conspicuous in the fingerling, pond-size, and adult; it was assumed to be coded by locus C. A negatively charged band, X4, was detected in fully developed ovary and in fry homogenized as whole individuals, but it could not be resolved in tissues of fingerling. Six-mo old stunts and 3-mo old fingerlings had similar LDH patterns for all tissues examined. The patterns for 11-mo old stunts and fingerlings also were similar but the one for the eye of the former was the same pattern resolved for the eye of adults. There was no change in the LDH isozyme patterns of milk fish stunted for 6 mo under different salinity levels (0-5, 15-20, 32-35 ppt). -
Book
Intensive culture of milkfish Chanos chanos in polyculture with white shrimp Penaeus indicus or mud crab Scylla serrata in brackishwater earthen ponds
- 2014 - Aquaculture Department, Southeast Asian Fisheries Development Center
Series: Aquaculture extension manual; No. 57A 30-page extension manual describing the biology, site selection, pond management and harvest & post-harvest of milkfish intensive polyculture in earthen pond.
