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The in vitro effects of cyclic nucleotides, cyanoketone, and cycloheximide on the production of estradiol-17β by vitellogenic ovarian follicles of goldfish (Carassius auratus)

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Date
1986
Author
Tan, Josefa D.
Adachi, Shinji
Nagahama, Yoshitaka
Page views
1,822
Subject
vitellogenesis ASFA
steroids ASFA
nucleotides ASFA
biosynthesis ASFA
ovaries ASFA
hormones ASFA
Carassius auratus AGROVOC
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Abstract
The effects of human chorionic gonadotropin (HCG), forskolin, cyclic nucleotides, the phosphodiesterase inhibitors IBMX and theophylline, cyanoketone, and cycloheximide on the production of estradiol-17β by isolated ovarian follicles of vitellogenic goldfish (Carassius auratus) were examined using 18-hr incubations. HCG and all test agents which are known to increase intracellular concentrations of cAMP significantly stimulated the production of estradiol-17β. However, dibutyryl cGMP was unable to stimulate estradiol-17β production at any concentration used (1–10 mM). Cyanoketone at a concentration of 1 μg/ml completely blocked forskolin-induced estradiol-17β production. Even in the presence of cyanoketone, however, forskolin stimulated conversion of exogenous testosterone to estradiol-17β in a dose-dependent manner, suggesting the involvement of an adenylate-cyclase system in the induction of aromatase activation by vitellogenic follicles of goldfish. Cycloheximide also completely abolished HCG-induced estradiol-17β production when this inhibitor was added within the first 1 hr after the addition of HCG. These results provide evidence that the stimulation of estradiol-17β by goldfish vitellogenic follicles in response to HCG is dependent upon the synthesis of new protein.
URI
http://hdl.handle.net/10862/1200
Suggested Citation
Tan, J. D., Adachi, S., & Nagahama, Y. (1986). The in vitro effects of cyclic nucleotides, cyanoketone, and cycloheximide on the production of estradiol-17β by vitellogenic ovarian follicles of goldfish (Carassius auratus). General and Comparative Endocrinology, 63(1), 110-116. https://doi.org/10.1016/0016-6480(86)90188-7 
DOI
10.1016/0016-6480(86)90188-7
Type
Article
ISSN
0016-6480
Collections
  • Journal Articles [1162]


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