Studies on the digestive lipases of milkfish, Chanos chanos
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Milkfish grown on two natural foods were examined to determine the distribution pattern of the digestive lipases along the digestive tract and to identify the optimum condition for lipase activity. One food consisted of a biological complex of unicellular algae and diatoms (Food A) and the other consisted of fibrous filamentous green algae, predominantly Chaetomorpha brachygona (Food B). The major sites of lipase secretion in milkfish digestive tract were the intestines, pancreas and pyloric caeca. Lipase activity was somewhat higher for fish grown on Food A than those grown on Food B. Intestinal lipase activity was observed to be maximal at 45°C and at pH 6.8 and 8.0. Activity of pancreatic lipase was observed to be maximal at 50°C and at pH 6.4 and 8.6. The detection of two well-defined pH optima, one at slightly acidic and the other at alkaline pH for both the intestinal and pancreatic lipases suggests a physiological versatility for lipid digestion in milkfish.
CitationBorlongan, I. G. (1990). Studies on the digestive lipases of milkfish, Chanos chanos.
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Conference paperLV Benitez - In RD Fortes, LC Darvin & DL de Guzman (Eds.), Fish and crustacean feeds and nutrition : Proceedings of the seminar-workshop on fish and crustacean feeds and nutrition held on 25-26 February 1985 at UPV, Iloilo City, 1989 - Philippine Council for Aquatic and Marine Research and DevelopmentThis paper reviews recent work on milkfish nutrition. Substantial progress had been made towards understanding the digestive physiology of milkfish. Major enzaymes envolved in the digestions of carbohydrates, protein and lipids had been detected in the pyloric caece, intestines and pancreas of milkfish. The most active carbohydrates were involved in the hydrolysis of α - glocosidic bonds. Intestinal amylase activity consistently reached the peak at about noon when milkfish gut was full. This confirms that milkfish is s daytime feeder. No cellulase activity was detected in any region orf the digertive treat although the fish relies heavily algae and other plant source for food. Trypsin, chymotrypsin and general proteases were also detected in milkfish digestive tract. A powerful milkfish trypsin inhabitor was detected in the filementous algae, Chaetomorpha brachygona which is predominant species in lumot. Lipass in the pancreas and intestines had two pH optima, suggesting a physiologic versatility for lipid digestion in milkfish. There is a limit information on the nutrient requirement of milkfish. Most studies showed that milkfish fry has a dietary requirement of 40% protein, and 7-10 lipid. Studies on the protein-energy requirement of fingerlings suggested that 30-40% protein, 10% fat and 25% carbohydrates are required. Subsequent studies showed an optimum protein energy to total metabolizable energy ratio of 44.4%. Amino acid test diets for milkfish had been formulated to contain white fish meal, gelatin and approprate amino acid mix.
Lactate dehydrogenase isozyme patterns during the development of milkfish, (Chanos chanos (Forskal)) PD Requintina, LM Engle & LV Benitez -
Kalikasan, The Philippine Journal of Biology, 1981 - University of the Philippines at Los BañosPolyacrylamide disc gel electrophoresis was done to determine the lactate dehydrogenase (LDH) isozyme patterns for fry (5-3 mg), fingerling (6-12 g), pond-size (150-250 g) and adult (6-9 kg) milkfish. The patterns were tissue specific; the different tissues examined, viz., eye, liver, heart, and skeletal muscle had different expressions of LDH isozymes. The resolved patterns appeared to be products of LDH gene loci A, B, and C. Subunits A and B were present in all tissues. A4 and B4 were predominant in skeletal and heart muscle, respectively; the two associated non-randomly in vivo and formed only the heteropolymers A3B and AB3. A liver band, L4, was most conspicuous in the fingerling, pond-size, and adult; it was assumed to be coded by locus C. A negatively charged band, X4, was detected in fully developed ovary and in fry homogenized as whole individuals, but it could not be resolved in tissues of fingerling. Six-mo old stunts and 3-mo old fingerlings had similar LDH patterns for all tissues examined. The patterns for 11-mo old stunts and fingerlings also were similar but the one for the eye of the former was the same pattern resolved for the eye of adults. There was no change in the LDH isozyme patterns of milk fish stunted for 6 mo under different salinity levels (0-5, 15-20, 32-35 ppt).
ArticleThe study was conducted in twelve 144-m2 ponds to evaluate the effect of different organic and inorganic fertilizers on the growth, survival, gross production, and profitability of marketable milkfish. The following treatments were used: Treatment I, SEAFDEC traditional fertilization practice (16-20-0 at 50 kg/ha and 45-0-0 at 15 kg/ha); Treatment II, half-dosage of Treatment I; Treatment III, chicken manure at 0.5 ton/ha; and Treatment IV, MASA (processed from agricultural and industrial wastes) fertilizer at 0.5 ton/ha. All treatments were applied once in every 2 weeks. No significant difference (P > 0.05) existed in the harvest and production of milkfish among the treatments. However, economic indicators such as return-on-investment (ROI), payback period, and marginal analysis ranked the performance of the fertilizer treatments in the order of I, II, III and IV. Fish kills occurred in three ponds applied with chicken manure and MASA fertilizer. This could have been due to a heavy build-up of organic matter in the pond bottom which led to the collapse of the benthic algal community, depletion of dissolved oxygen and the presence of hydrogen sulfide. It is therefore suggested that a lower dosage of organic fertilizer should be applied in ponds especially during the rainy season.