Development of an ovarian biopsy technique in the sea bass, Lates calcarifer (Bloch).
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A convenient and rapid biopsy method for taking ovarian samples from mature sea bass (Lates calcarifer Bloch) is described. Intra-ovarian oocytes siphoned with polyethylene tubing from any region of the ovary provide a sample representative of the maturational stage of sea bass. The osmolality of a 5% phosphate buffered formalin solution is similar to that of sea bass plasma. The follicular diameter of cannulated sea bass oocytes can be measured within an hour after fixation in 5% phosphate buffered formalin without significantly deviating from the diameter of fresh oocytes.
Garcia, L. (1989). Development of an ovarian biopsy technique in the sea bass, Lates calcarifer (Bloch).
Aquaculture, 77(1), 97-102. https://doi.org/10.1016/0044-8486(89)90025-2
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