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dc.contributor.authorToledo, Jeobert D.
dc.contributor.authorKurokura, Hisashi
dc.date.accessioned2013-01-22T03:35:43Z
dc.date.available2013-01-22T03:35:43Z
dc.date.issued1990
dc.identifier.citationToledo, J. D., & Kurokura, H. (1990). Cryopreservation of the euryhaline rotifer Brachionus plicatilis embryos. Aquaculture, 91(3–4), 385–394.en
dc.identifier.issn0044-8486
dc.identifier.urihttp://hdl.handle.net/10862/1323
dc.description.abstractA method for the separation and cryopreservation of Brachionus plicatilis embryos is described. Juveniles with uniform development were collected from a cultured stock by passing them through a series of nets. Collected juveniles were cultured and the embryos separated by vigorous vortex mixing as soon as the majority had laid their first eggs. Separated embryos at stage I (cleavage stage), stage II (invagination stage), stage III (symmetrical embryo stage), or stage IV (“eyed” stage) were frozen to −196°C using various concentrations of DMSO and a two-step freezing procedure. No stage I embryos survived freezing and the highest post-thaw survival was obtained with stage III embryos. A DMSO concentration of 10% of the freezing medium resulted in high post-thaw survival while concentrations higher than 10% appeared to be harmful to embryos. Prolonged incubation in 10% DMSO for up to 30 min before freezing increased post-thaw survival. Incorporating the above results, stage III embryos from a single batch culture were incubated in 10% DMSO for 30 min and frozen to −196°C. Post-thaw survival rates of 63%, 62%, 53%, and 55% were obtained after 3, 7, 15, and 30 days of storage in liquid nitrogen, respectively. Survivors fed actively on marine chlorella and started to lay eggs 2–3 days after thawing.en
dc.language.isoenen
dc.publisherElsevieren
dc.subjectBrachionus plicatilis
dc.titleCryopreservation of the euryhaline rotifer Brachionus plicatilis embryosen
dc.typeArticleen
dc.identifier.doi10.1016/0044-8486(90)90202-X
dc.citation.volume91
dc.citation.issue3-4
dc.citation.spage385
dc.citation.epage394
dc.citation.journalTitleAquacultureen
seafdecaqd.library.callnumberVF SJ 0307
seafdecaqd.databank.controlnumber1990-29
dc.subject.asfaAquaculture techniquesen
dc.subject.asfaCryobiologyen
dc.subject.asfaEmbryosen
dc.subject.asfaFreezing storageen
dc.subject.asfaSurvivalen
dc.subject.asfaThawingen


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