Spermiation response of mature rabbitfish, Siganus guttatus Bloch, to luteinizing hormone-releasing hormone analogue (LHRHa) injection
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A method to quantify the spermiation response of mature rabbitfish to hormonal therapy is described. Spermatocrit or packed sperm volume was measured after milt loaded in non-heparinized microhematocrit capillary tubes was centrifuged for 45 min at 15 000 × g. Sperm density was estimated by a standard hemacytometer method. However, sperm density can be predicted from spermatocrit since their relationship is highly and positively correlated (Y = 29.5 + 2.95 Xr = 0.83; P < 0.01). Milt production by mature rabbitfish was highest (4.9 ml per kg body weight) at 24 h after injection of 20 μg of luteinizing hormone-releasing hormone analogue (D-Ala6, Pro9-LHRH-ethylamide) per kg body weight and coincided with low spermatocrit (68%) and sperm density (14.1 × 106 spermatozoa per μl milt) levels. These results demonstrate that spermiation in mature rabbitfish can be reliably assessed by a spermatocrit method and that LHRHa can effectively stimulate spermiation in this species.
Garcia, L. (1991). Spermiation response of mature rabbitfish, Siganus guttatus Bloch, to luteinizing hormone-releasing hormone analogue (LHRHa) injection.
Aquaculture, 97(2-3), 291-299. https://doi.org/10.1016/0044-8486(91)90271-8
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