Natural spawning, egg and fry production of milkfish, Chanos chanos (Forsskal), broodstock reared in concrete tanks
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Gonadal maturation, rematuration, and spawning of nine- (1982 stock) and ten-year-old (1981 stock) milkfish reared in 150 and 200 m3 concrete tanks were observed in 1990 and 1991. From 23 September to 9 November 1990, the 1981 stock spawned 15 times with an average daily collection of 61,000 ± 21,000 eggs. From 4 larval rearing trials, the mean overall survival rate (from eggs to 21 -day-old fry) was 9.68 ± 4.3%. From 25 Aril to 31 October 1991, the stock spawned a totaf of 108 times. Mean daily egg collection increasef to 305,000 ± 32,000. Successful rearing trials conducted during the latter part of the spawning season had a mean overall survival rate of 17 ± 3% (n = 17) indicating that mass fry production techniques can be improved. Spawning of milkfish broodstock in concrete tanks provides an alternative to other existing methods of seed production.
Preliminary results were partially reported in SEAFDEC Asian Aquaculture 12(4): 1-3 (1990).
CitationEmata, A. C., & Marte, C. L. (1994). Natural spawning, egg and fry production of milkfish, Chanos chanos (Forsskal), broodstock reared in concrete tanks.
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Lactate dehydrogenase isozyme patterns during the development of milkfish, (Chanos chanos (Forskal)) PD Requintina, LM Engle & LV Benitez -
Kalikasan, The Philippine Journal of Biology, 1981 - University of the Philippines at Los BañosPolyacrylamide disc gel electrophoresis was done to determine the lactate dehydrogenase (LDH) isozyme patterns for fry (5-3 mg), fingerling (6-12 g), pond-size (150-250 g) and adult (6-9 kg) milkfish. The patterns were tissue specific; the different tissues examined, viz., eye, liver, heart, and skeletal muscle had different expressions of LDH isozymes. The resolved patterns appeared to be products of LDH gene loci A, B, and C. Subunits A and B were present in all tissues. A4 and B4 were predominant in skeletal and heart muscle, respectively; the two associated non-randomly in vivo and formed only the heteropolymers A3B and AB3. A liver band, L4, was most conspicuous in the fingerling, pond-size, and adult; it was assumed to be coded by locus C. A negatively charged band, X4, was detected in fully developed ovary and in fry homogenized as whole individuals, but it could not be resolved in tissues of fingerling. Six-mo old stunts and 3-mo old fingerlings had similar LDH patterns for all tissues examined. The patterns for 11-mo old stunts and fingerlings also were similar but the one for the eye of the former was the same pattern resolved for the eye of adults. There was no change in the LDH isozyme patterns of milk fish stunted for 6 mo under different salinity levels (0-5, 15-20, 32-35 ppt).
Conference paperLV Benitez - In RD Fortes, LC Darvin & DL de Guzman (Eds.), Fish and crustacean feeds and nutrition : Proceedings of the seminar-workshop on fish and crustacean feeds and nutrition held on 25-26 February 1985 at UPV, Iloilo City, 1989 - Philippine Council for Aquatic and Marine Research and DevelopmentThis paper reviews recent work on milkfish nutrition. Substantial progress had been made towards understanding the digestive physiology of milkfish. Major enzaymes envolved in the digestions of carbohydrates, protein and lipids had been detected in the pyloric caece, intestines and pancreas of milkfish. The most active carbohydrates were involved in the hydrolysis of α - glocosidic bonds. Intestinal amylase activity consistently reached the peak at about noon when milkfish gut was full. This confirms that milkfish is s daytime feeder. No cellulase activity was detected in any region orf the digertive treat although the fish relies heavily algae and other plant source for food. Trypsin, chymotrypsin and general proteases were also detected in milkfish digestive tract. A powerful milkfish trypsin inhabitor was detected in the filementous algae, Chaetomorpha brachygona which is predominant species in lumot. Lipass in the pancreas and intestines had two pH optima, suggesting a physiologic versatility for lipid digestion in milkfish. There is a limit information on the nutrient requirement of milkfish. Most studies showed that milkfish fry has a dietary requirement of 40% protein, and 7-10 lipid. Studies on the protein-energy requirement of fingerlings suggested that 30-40% protein, 10% fat and 25% carbohydrates are required. Subsequent studies showed an optimum protein energy to total metabolizable energy ratio of 44.4%. Amino acid test diets for milkfish had been formulated to contain white fish meal, gelatin and approprate amino acid mix.
Milkfish (Chanos chanos) fingerling production in freshwater ponds with the use of natural and artificial feeds Milkfish fry were reared to fingerling size in freshwater ponds. For the first experiment, fish were fed the blue-green algae Oscillatoria inoculated and grown in the ponds, Oscillatoria supplemented with a fishmeal-based formulated diet, and the formulated diet alone. Twelve 50-m2 earthen ponds were prepared to enhance growth of the indigenous natural foods. Acclimated wild milkfish fry were stocked randomly at 90/m2 and were fed for 6 weeks. Milkfish fed the formulated diet alone had a significantly higher (P<0.05) mean weight gain (1.314±0.201 g) than milkfish given the combination of Oscillatoria and formulated diet (0.882±0.230 g). Growth was lowest for fish fed Oscillatoria alone. The feeding treatments in the second experiment were: combination of Spirulina powder and formulated diet, formulated diet alone, and rice bran alone. The stocking rate was equivalent to 91.5–92.5 fry/m2 and feeding lasted for 7 weeks. All feeds promoted some growth but the milkfish fed the formulated diet alone invariably had the highest weight increment (1.504±0.167 g), followed by fish given the feed combination (0.881±0.140 g). Rice bran alone gave the lowest growth response. For both pond experiments, growth trends of the young milkfish were similar to those grown under laboratory conditions. Although survival rates were significantly different in one aquarium experiment, survival rates of milkfish in ponds did not differ significantly (P>0.05) among treatments.