Resistance of rainbow trout Oncorhynchus mykiss to infectious hematopoietic necrosis virus (IHNV) experimental infection following ingestion of natural and synthetic carotenoids
MetadataShow full item record
Cited times in Scopus
Further to previous studies showing modulation of innate immune responses by dietary carotenoids, an experiment was conducted to examine the resistance to a viral pathogen in rainbow trout after oral ingestion of synthetic or natural carotenoids. Rainbow trout fry weighing 0.11 g on average (n = 30) were fed casein-based semi-purified diets supplemented with 100 mg carotenoids kg− 1 diet for 6 weeks. The synthetic sources tested were pure β-carotene, astaxanthin, and canthaxanthin, whereas the natural sources were Dunaliella salina, Phaffia rhodozyma, Tagetes erecta, and Capsicum annuum. No significant differences (P > 0.05) in growth and feed performance were found among the groups after 6 weeks of feeding. Subsequently, fish were challenged by immersion in two concentrations (2 × 103 and 2 × 104 TCID50 ml− 1) of a virulent strain of infectious hematopoietic necrosis virus (IHNV) and cumulative mortalities were recorded over a 30-day period. No significant differences in survival (P > 0.05) were found among the groups when challenged with the high viral dose. However, at the lower viral dose, mortality was markedly reduced in fish fed astaxanthin (22%). Consequently, this group exhibited the highest relative percent survival (RPS) of 58%, which was significantly different from the control (P < 0.05). Rainbow trout fed D. salina and T. erecta among the natural sources, had reduced mortality rates and elevated RPS that, nonetheless, did not significantly differ from the control. This study highlights the influence of carotenoids, particularly astaxanthin, in maintaining fish health and disease resistance.
CitationAmar, E. C., Akutsu, T., Satoh, S., & Watanabe, T. (2012). Resistance of rainbow trout Oncorhynchus mykiss to infectious hematopoietic necrosis virus (IHNV) experimental infection following ingestion of natural and synthetic carotenoids.
Roche Vitamins Japan, KK, gifted the synthetic carotenoids employed in the study. The assistance of Mr. Yoshihiro Yasuda and Mr. Akihiro Harakawa of the Yoshida Research Station of Tokyo University of Marine Science and Technology (TUMSAT) is gratefully acknowledged. We are thankful to Dr. Hideo Fukuda of the Fish Disease Laboratory for allowing the use of experimental infection facilities and to Dr. Nobuaki Okamoto of the Fish Physiology Laboratory for fruitful discussions. This study was partly supported by the Ministry of Education, Culture, Sports, Science and Technology of Japan (MEXT). SEAFDEC AQD is acknowledged for providing paid leave of absence to the first author.
- Journal Articles