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dc.contributor.authorAyson, Felix G.
dc.contributor.authorde Jesus-Ayson, Evelyn Grace T.
dc.contributor.authorTakemura, Akihiro
dc.date.accessioned2013-11-12T10:20:25Z
dc.date.available2013-11-12T10:20:25Z
dc.date.issued2007
dc.identifier.citationAyson, F. G., de Jesus-Ayson, E. G. T., & Takemura, A. (2007). mRNA expression patterns for GH, PRL, SL, IGF-I and IGF-II during altered feeding status in rabbitfish, Siganus guttatus. General and Comparative Endocrinology, 150(2), 196-204.en
dc.identifier.issn0016-6480
dc.identifier.urihttp://hdl.handle.net/10862/1720
dc.description.abstractFeeding time is a major synchronizer of many physiological rhythms in many organisms. Alteration in the nutritional status, specifically fasting, also affects the secretion rhythms of growth hormone (GH) and insulin-like growth factor-I (IGF-I). In this study, we investigated whether the expression patterns for the mRNAs of GH, prolactin (PRL) and somatolactin (SL) in the pituitary gland, and insulin-like growth factor I and II (IGF-I and IGF-II) in the liver of juvenile rabbitfish (Siganus guttatus) follow a rhythm according to feeding time and whether these hormone rhythms changes with starvation. Hormone mRNA levels were determined by real time PCR. The daily expression pattern for the mRNAs of GH, PRL and SL was not altered whether food was given in the morning (10:00 h) or in the afternoon (15:00 h). The daily GH mRNA expression pattern, however, was affected when food was not available for 3 days. In contrast, the daily expression pattern for IGF-I mRNA reaches its peak at roughly 5–6 h after feeding. This pattern, however, was not observed with IGF-II mRNA. During 15-day starvation, GH mRNA levels in starved fish were significantly higher than the control fish starting on the 9th day of starvation until day 15. The levels returned to normal after re-feeding. In contrast to GH, PRL mRNA levels in starved fish were significantly lower than the control group starting on the 6th day of starvation until 3 days after re-feeding. SL mRNA levels were not significantly different between the control and starved group at anytime during the experiment. Both IGF-I and IGF-II mRNA levels in starved group were significantly higher than the control fish on the 3rd and 6th day of starvation. mRNA levels of both IGF-I and II in the starved fish decreased starting on the 9th day of starvation. While IGF-I mRNA levels in the starved group continued to decrease as starvation progressed, IGF-II mRNA levels were not significantly different from the control during the rest of the starvation period. The results indicate that aside from GH and IGF-I, PRL and IGF-II are likewise involved in starvation in rabbitfish.en
dc.language.isoenen
dc.publisherElsevieren
dc.subjectSiganus guttatusen
dc.subjectGrowth hormoneen
dc.subjectProlactinen
dc.subjectSomatolactinen
dc.subjectInsulin-like growth factor-Ien
dc.subjectInsulin-like growth factor-IIen
dc.subjectRabbitfishen
dc.subjectmRNAen
dc.subjectFeeding timeen
dc.subjectReef fishen
dc.subjectRhythmsen
dc.subjectFastingen
dc.subjectNutritional statusen
dc.titlemRNA expression patterns for GH, PRL, SL, IGF-I and IGF-II during altered feeding status in rabbitfish, Siganus guttatus.en
dc.typeArticleen
dc.identifier.doi10.1016/j.ygcen.2006.08.001
dc.citation.volume150
dc.citation.issue2
dc.citation.spage196
dc.citation.epage204
dc.citation.journalTitleGeneral and Comparative Endocrinologyen
seafdecaqd.databank.controlnumber2007-07
dc.subject.asfaFeeding behaviouren
dc.subject.asfaGrowth rateen
dc.subject.asfaHormonesen
dc.subject.asfaNutritionen
dc.subject.asfaPituitary glanden
dc.subject.asfaReef fishen
dc.subject.asfaSecretionen
dc.subject.asfaStarvationen
dc.subject.asfaFeedingen
dc.subject.asfaGene expressionen
dc.subject.asfaFood availabilityen
dc.subject.asfaLiveren
dc.subject.asfaFooden
dc.subject.asfaPolymerase chain reactionen


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