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dc.contributor.authorHamid, A. A. A.
dc.contributor.authorHamdan, S.
dc.contributor.authorPakingking Jr., Rolando V.
dc.contributor.authorHuyop, F.
dc.date.accessioned2013-11-13T06:33:21Z
dc.date.available2013-11-13T06:33:21Z
dc.date.issued2010
dc.identifier.citationHamid, A. A. A., Hamdan, S., Pakingking, R. V., & Huyop, F. (2010). Identification of Pseudomonas sp. strain S3 based on small subunit ribosomal RNA gene sequences. Biotechnology, 9(1), 33-40.en
dc.identifier.issn1682-296X
dc.identifier.urihttp://hdl.handle.net/10862/1724
dc.descriptionOpen access article.en
dc.description.abstractPseudomonas sp. strain S3 was isolated from Paddy (rice) field agricultural area. This organism, which can utilize a halogenated compound of D,L-2-Chloropropionic acid as sole carbon and energy source, catalyses the hydrolytic dehalogenation of both D- and L- isomers of 2-Chloropropionic acid. Identification of Pseudomonas sp. S3 is still ambiguous due to the lack of basic studies, especially their molecular genetic information. In this study, the amplified 16S rRNA gene sequence of Pseudomonas sp. S3 (Accession No. FJ968758) was compared to other nine selected gene sequences from the same group of Pseudomonas sp. and/or dehalogenase producing bacteria using in silico method. Their phylogenetic relationships were then determined. The results were analysed using MEGA4 software to ascertain its evolutionary distance by reconstructing a phylogenetic tree of these organisms. The evolutionary history and bootstrap consensus tree were inferred using the Neighbour-Joining method from 500 replicates. The tree is drawn to scale, with branch lengths (next to the branches) in the same units as those of the evolutionary distances used to infer the phylogenetic tree. The evolutionary distances were computed using the p-distance method and were in the units of the number of base substitutions per site. Based on this analysis, Pseudomonas sp. S3 16S rRNA gene was closely related to the Pseudomonas chlororaphis with genetic distance 0.170 base substitutions per site. S3 gene was also compared among known dehalogenase producing bacteria 16S rRNA genes. Results suggested that S3 was closely related to the Pseudomonas sp. R1 with a genetic distance 0.040 base substitutions per site. From present study, evolutionary relationships of 16S rRNA gene of Pseudomonas sp. S3 were elegantly illustrated by phylograms, comparable to a pedigree showing which microorganisms are most closely related.en
dc.description.sponsorshipThis study was partly supported by Fundamental Research Grant Schemes 78307 from Ministry of Higher Education (MOHE) Malaysia.en
dc.language.isoenen
dc.publisherAsian Network for Scientific Informationen
dc.relation.urihttp://www.scialert.net/qredirect.php?doi=biotech.2010.33.40&linkid=pdf
dc.subjectOryza sativaen
dc.subjectPseudomonas chlororaphisen
dc.subjectPedigreeen
dc.subjectIsomersen
dc.subjectComputer softwareen
dc.subjectDehalogenationen
dc.subjectGenetic distanceen
dc.titleIdentification of Pseudomonas sp. strain S3 based on small subunit ribosomal RNA gene sequences.en
dc.typeArticleen
dc.identifier.doi10.3923/biotech.2010.33.40
dc.citation.volume9
dc.citation.issue1
dc.citation.spage33
dc.citation.epage40
dc.citation.journalTitleBiotechnologyen
seafdecaqd.databank.controlnumber2010-
dc.subject.asfaGenesen
dc.subject.asfaMicroorganismsen
dc.subject.asfaNucleotide sequenceen
dc.subject.asfaPhylogeneticsen
dc.subject.asfaRNAen
dc.subject.asfaRice fieldsen
dc.subject.asfaPhylogenyen
dc.subject.asfaComputer programsen
dc.subject.asfaCarbonen
dc.subject.asfaEnergyen


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