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dc.contributor.authorLio-Po, Gilda D.
dc.contributor.authorTraxler, G. S.
dc.contributor.authorAlbright, L. J.
dc.date.accessioned2013-12-16T02:50:03Z
dc.date.available2013-12-16T02:50:03Z
dc.date.issued1999
dc.identifier.citationLio-Po, G. D., Traxler, G. S., & Albright, L. J. (1999). Establishment of cell lines from catfish (Clarias batrachus) and snakeheads (Ophicephalus striatus). Asian Fisheries Science, 12(4), 343–349.en
dc.identifier.issn0116-6514
dc.identifier.urihttp://hdl.handle.net/10862/1839
dc.description.abstractPrimary cell cultures from catfish (Clarias batrachus) and snakeheads (Ophicephalus striatus) were prepared from whole fry and fingerling organ tissues of the brain, fins, gonad, heart, kidney, liver, skin and spleen. Four methods were tried: method A, wherein explants were placed onto the surface of 25-cm2 Primaria flasks (Falcon), allowed to attach for an hour before addition of Leibovitz medium (L-15) supplemented with 15% fetal bovine serum (FBS)(L15-15); Method B, wherein explants were inoculated into 25-cm2 Primaria flasks (Falcon) already containing L15-15; Method C, which required forcing minced organ sections through a stainless steel sieve with the aid of a syringe plunger into a petri dish containing L15-15 medium; and Method D, wherein immersed sections of minced tissues to 0.5% trypsin-EDTA were slowly agitated using a magnetic stirrer for one hour at 25°C. Method B was most effective in the establishment of cell cultures from both fish species. Passage numbers of the cells are to date catfish gonad (CFG) P-56, catfish heart (CFH) P-51, catfish kidney (CFK) P-7, catfish liver (CFL) P-8, catfish spleen (CFS) P-54, snakehead gonad (SHG) P-26, snakehead heart (SHH) P-22, snakehead kidney (SHK) P-19, snakehead liver (SHL) P-49 and snakehead spleen (SHS) P-76. Attempts to derive primary cell cultures from organ tissues of the brain, fins, skin and whole fry were unsuccessful. Established cells were fibroblastic. The cells grew rapidly and became confluent 24 h after seeding at 20 and 25°C. Both SHS and CFS were susceptible to a virus isolated from EUS-affected fish in the Philippines. The cells were best maintained at 20°C and stored in liquid nitrogen or -70°C.en
dc.description.sponsorshipThe authors wish to thank the Aquaculture Department, Southeast Asian Fisheries Development Center and the International Development Research Centre (IDRC) of Canada (Fish Microbiology Project 3-P-88-1053-02) for funding support. Appreciation is also due to Dr. R. Hedrick, Univ. Calif.-Davis, for his technical suggestions and to Dr. K. Tonguthai through the SEAFDEC Project for funding assistance in attending the 5th Asian Fisheries Forum held in Chiangmai, Thailand, in Nov. 1998.en
dc.language.isoenen
dc.publisherAsian Fisheries Societyen
dc.relation.urihttp://www.asianfisheriessociety.org/publication/pdf/0408672001355820748.pdf
dc.subjectClarias batrachusen
dc.subjectChanna striatusen
dc.titleEstablishment of cell lines from catfish (Clarias batrachus) and snakeheads (Ophicephalus striatus)en
dc.typeArticleen
dc.citation.volume12
dc.citation.issue4
dc.citation.spage343
dc.citation.epage349
dc.citation.journalTitleAsian Fisheries Scienceen
seafdecaqd.library.callnumberVF SJ 0612
seafdecaqd.databank.controlnumber1999-29
dc.subject.asfacell cultureen
dc.subject.asfadisease resistanceen
dc.subject.asfafish diseasesen
dc.subject.asfatissue cultureen
dc.subject.scientificNameOphicephalus striatus
dc.subject.scientificNameChanna striata


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  • Journal Articles [1225]
    These papers were contributed by Department staff to various national and international journals.

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