Measures of egg quality in induced spawns of the Asian sea bass, Lates calcarifer Bloch
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The egg morphometry and lipid and protein components were determined in induced spawns (n = 14) of the sea bass, Lates calcarifer, to identify measures of egg quality. Based on fertilization and hatching rates, the spawns were classified either in Group I, (zero fertilization) or Group II (where fertilization and hatching occurred). The egg morphometry did not differ between the two groups (p > 0.05). The total lipid was higher in Group II than in Group I, although the difference was not significant (p > 0.05). The EPA and linoleic acid were significantly higher in Group II (p < 0.05). There were positive correlations between the total saturated fatty acids and fertilization rate (p < 0.05; r = 0.58), the total saturated fatty acids and percentage of normal zygotes (p < 0.02; r = 0.62), and DHA and the percentage of normal zygotes (p < 0.04; r = 0.56). The total protein and FAAs were higher in Group I than in Group II, but the differences were not significant (p > 0.05). Proline, glycine, p-ethanolamine, and aspartic acid were significantly higher in Group II (p < 0.05), while tyrosine and glutamic acid were significantly higher in Group I (p < 0.05). Phosphoserine and fertilization rate were positively correlated (p < 0.03; r = 0.60), as well as aspartic acid and hatching rate (p < 0.05; r = 0.54). Arginine was negatively correlated with fertilization rate (p < 0.03; r = -0.61) and the percentage of normal zygotes (p < 0.03; r = -0.63). Serine was inversely correlated with yolk volume of the newly-hatched larvae (p < 0.03; r = -0.77). The moisture content of the eggs, which was significantly higher in Group II than in Group I (p < 0.03), was directly correlated with the FAAs:protein ratio (p < 0.03; r = 0.76). The present results reveal egg components that may be used as quality measures in induced spawns of sea bass, a euryhaline teleost that spawn pelagic eggs containing an oil globule.
CitationNocillado, J. N., Peñaflorida, V. D., & Borlongan, I. G. (2000). Measures of egg quality in induced spawns of the Asian sea bass, Lates calcarifer Bloch.
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Conference paperMH Carlos & CB Santiago - In JV Juario & LV Benitez (Eds.), Seminar on Aquaculture Development in Southeast Asia, 8-12 September 1987, Iloilo City, Philippines, 1988 - Aquaculture Department, Southeast Asian Fisheries Development CenterMost researches conducted at the Binangonan Freshwater Station of the SEAFDEC Aquaculture Department were directed toward enhancing growth and survival of the young tilapia and carp in the nursery as well as increasing yields in grow-out cages, pens, and ponds. Studies included the culture and evaluation of phytoplankton and zooplankton as feeds of the tilapia and carp fry to fingerlings; determination of protein and amino acid requirements of young Nile tilapia; development of practical dry diets; evaluation of feeding regimes, feeding rates, and feeding frequencies ; and the use of fertilizers in nursery ponds. For the grow-out aspect, one of the earliest studies demonstrated the profitability of the monoculture of tilapia in cages which triggered the initial proliferation of tilapia cage culture by the private sector in areas near the Station. Subsequently, supplemental feeds were developed and evaluated; non-conventional feedstuffs were tested as feeds or feed components; and the growth rates of Nile tilapia fingerlings in cages at varying stocking densities were evaluated at three distinct rearing periods covering one year. Prior to the successful mass production of bighead carp fingerlings at the Station, studies on polyculture of tilapia, milk fish, and different species of carp were conducted in cages and pens with remarkable results. This led to the technology-verification projects on polyculture at various areas in Laguna Lake. With the availability of freshwater fishponds for research purposes, studies on polyculture in ponds were also conducted.
Conference paperAC Fermin - In JV Juario & LV Benitez (Eds.), Seminar on Aquaculture Development in Southeast Asia, 8-12 September 1987, Iloilo City, Philippines, 1988 - Aquaculture Department, Southeast Asian Fisheries Development CenterBighead (Aristichthys nobilis) and silver (Hypophthalmichthys molitrix) carps were reared in ponds, pens and floating cages in Laguna Lake until maturity. Spontaneous gonadal maturation and rematuration of carp broodstock occurred within 2-2.5 years with average weight of 3-4 kg. Under lake conditions, broodstock were not given supplemental feeds. Induced spawning of gravid females was done by intraperitoneal injections using HCG combined with either common carp pituitary homogenates or LHRH-A. Stripping and dry-fertilization of eggs were done 6-8 hr after the final injection. Eggs were incubated in water containing 300-500 ppm Total Hardness. Fertilization and hatching rates were 23-88% and 7-36%, respectively. Post-larval carps were reared in tanks and fine-meshed nylon net cages installed in manured ponds. Tank-reared post-larvae were fed with Brachionus plicatilis and subsequently with Moina macrocopa in combination with powered formulated feeds containing 40% crude protein. Fry were harvested and stocked in nursery cages after 30-45 days of rearing in tanks. Four-month old 50-100 g tilapia (Oreochromis niloticus) stocked in hapa net cages, tanks or ponds were used for breeding. Egg and fry production was significantly high at 4 females/m2 stocking density Different sex ratios, however, did not affect fry production. Spawning frequency and total growth of broodstock was highest in fry fed formulated diets containing 50% crude protein. Harvesting of fry was done every 15 days during summer months and every 21 days during cold months. Fry were reared in tanks and hapa cages and fed diets containing 35% crude protein. Supplemental feeding in the lake was suspended when productivity reached 3 gC/m2 /day.
Conference paperHM Ali - In CL Marte, GF Quinitio & AC Emata (Eds.), Proceedings of the Seminar-Workshop on Breeding and Seed Production of Cultured Finfishes in the Philippines, Tigbauan, Iloilo, Philippines, 4-5 May 1993, 1996 - Aquaculture Department, Southeast Asian Fisheries Development CenterResearch on grouper (Epinephelus suillus) fry production in captivity has been carried out in Malaysia since 1986 at Tanjung Demong Marine Finfish Production and Research Centre (TDMFPRC) but the breakthrough was only achieved four years later in 1990. Eggs were obtained through natural and induced spawning in tanks. Natural spawning of grouper in captivity seldom occurred and was unpredictable. However induced spawnings were successfully carried out by injecting human chorionic gonadotropin (hCG) intramuscularly at a dose of 500-1000 IU/kg fish. The results from several trials on larval rearing conducted since 1989 until recently showed that larvae obtained from natural spawnings survived longer with some reaching the juvenile stage. The highest recorded survival rate of 43 days posthatch (32.5 mm total length) was 12.1% at 28-32 °C water temperature. On the other hand, 100% mortality usually occurred in larvae obtained from induced spawning 7 days after hatching. The major constraints of grouper fry production in Malaysia are lack of male spawners, inconsistent and unpredictable natural spawning, small quantity of eggs released every spawning day, poor fertilization and hatching rate, weak hatchlings, and high mortality rate at the early stages of larval development. The latter is probably due mainly to problems on initial feeding.