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dc.contributor.authorCatap, Elena S.
dc.contributor.authorMunday, Barry L.
dc.date.accessioned2014-01-17T08:33:28Z
dc.date.available2014-01-17T08:33:28Z
dc.date.issued2002
dc.identifier.citationCatap, E. S., & Munday, B. L. (2002). Development of a method for reproducing epizootic ulcerative syndrome using controlled doses of Aphanomyces invadans in species with different salinity requirements. Aquaculture, 209(1-4), 35-47.en
dc.identifier.issn0044-8486
dc.identifier.urihttp://hdl.handle.net/10862/1922
dc.description.abstractLesions typical of epizootic ulcerative syndrome (EUS) were induced in three-spot gourami, Trichogaster trichopterus, and sand whiting, Sillago ciliata, injected intramuscularly with controlled doses of Aphanomyces invadans zoospores, the fungal pathogen associated with the disease. Both species of fish exhibited chronic granulomatous response and inflammatory cells, predominantly macrophages and lymphocytes, infiltrated the muscle and skin tissues, at days 6–8 post-inoculation of 65 to 85 spores/fish. Based on the comparative granuloma counts and percentage of cellular infiltration in a sampled lesion area using image analysis, it was shown that the three-spot gouramis mounted a more vigorous response than the sand whiting. It was also observed that lesions in three-spot gouramis exhibited early signs of resolution than those in sand whiting. However, fish mortality was greater in EUS-affected three-spot gourami than in EUS-affected sand whiting. With this technique, we were able to describe and compare the sequential histopathology of EUS lesions in a freshwater (three-spot gourami) and an estuarine (sand whiting) fish species.en
dc.description.sponsorshipThe authors are grateful to the Australian Centre for International Agricultural Research for the financial assistance; the sand whiting were provided by the Bribie Island Aquaculture Research Centre, DPI Queensland while the A. invadans (24 P) isolate, originally isolated by Ms. Annette Thomas, was provided by Dr. R.B. Callinan; Mr. Craig Thomas provided technical assistance with the rearing of juvenile sand whiting.en
dc.language.isoenen
dc.publisherElsevieren
dc.subjectSillago ciliataen
dc.subjectTrichogaster trichopterusen
dc.subjectSand whitingen
dc.subjectThree-spot gouramien
dc.subjectEpizootic ulcerative syndromeen
dc.titleDevelopment of a method for reproducing epizootic ulcerative syndrome using controlled doses of Aphanomyces invadans in species with different salinity requirementsen
dc.typeArticleen
dc.identifier.doi10.1016/S0044-8486(01)00805-5
dc.citation.volume209
dc.citation.issue1-4
dc.citation.spage35
dc.citation.epage47
dc.citation.journalTitleAquacultureen
seafdecaqd.library.callnumberVF SJ 0706
seafdecaqd.databank.controlnumber2002-17
dc.subject.asfabrackishwater fishesen
dc.subject.asfadefence mechanismsen
dc.subject.asfafreshwater fishesen
dc.subject.asfafungal diseasesen
dc.subject.asfahistopathologyen
dc.subject.asfaimmunityen
dc.subject.asfalesionsen
dc.subject.asfalymphocytesen
dc.subject.asfamacrophagesen
dc.subject.asfasalinity effectsen
dc.subject.asfasporesen
dc.subject.asfamusclesen
dc.subject.asfaskinen
dc.subject.scientificNameAphanomyces invadansen


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  • Journal Articles [1222]
    These papers were contributed by Department staff to various national and international journals.

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