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dc.contributor.authorMaeno, Yukio
dc.contributor.authorde la Peña, Leobert D.
dc.contributor.authorCruz-Lacierda, Erlinda R.
dc.date.accessioned2014-05-09T04:03:41Z
dc.date.available2014-05-09T04:03:41Z
dc.date.issued2004
dc.identifier.citationMaeno, Y., de la Peña, L. D., & Cruz-Lacierda, E. R. (2004). Mass mortalities associated with viral nervous necrosis in hatchery-reared sea bass Lates calcarifer in the Philippines. Japan Agricultural Research Quarterly, 38(1), 69-73.en
dc.identifier.issn0021-3551
dc.identifier.urihttp://hdl.handle.net/10862/2016
dc.description.abstractViral nervous necrosis (VNN), also known as viral encephalopathy and retinopathy (VER), is an emerging disease affecting larvae and juveniles of many farmed marine fish species in Asia, Australia, Europe and North America. Mass mortality occurred in 14-day old larval sea bass Lates calcarifer at a hatchery in the Philippines associated with clinical signs such as abnormal swimming behavior and pale-gray discoloration of the body. Histological investigations in moribund fish revealed marked vacuolation in the retina and brain. Cytopathic effects (CPE) were observed in SSN-1 cells inoculated with the tissue filtrate of affected sea bass. A piscine nodavirus, the causative agent of VNN, was detected in the affected tissues and SSN-1 cells inoculated with the tissue filtrate of affected fish by RT-PCR. Electron microscopy revealed non-enveloped viral particles, 22-28 nm in diameter, in the cytoplasm of the brain and retina of affected fish and in the cytoplasm of VNN-infected SSN-1 cells after CPE appeared. These results indicate that mass mortality of sea bass larvae in the Philippines was caused by a piscine nodavirus.en
dc.language.isoenen
dc.publisherMinistry of Tropical Agricultural Research Centreen
dc.relation.urihttp://www.jircas.affrc.go.jp/english/publication/jarq/38-1/38-01-10.pdf
dc.subjectLates calcariferen
dc.subjectPhilippinesen
dc.subjectVNNen
dc.subjectVERen
dc.subjectPiscine nodavirusen
dc.titleMass mortalities associated with viral nervous necrosis in hatchery-reared sea bass Lates calcarifer in the Philippinesen
dc.typeArticleen
dc.identifier.doi10.6090/jarq.38.69
dc.citation.volume38
dc.citation.issue1
dc.citation.spage69
dc.citation.epage73
dc.citation.journalTitleJapan Agricultural Research Quarterlyen
seafdecaqd.library.callnumberVF SJ 0769
seafdecaqd.databank.controlnumber2004-16
dc.subject.asfaFish killen
dc.subject.asfaHatchingen
dc.subject.asfaInfectious diseasesen
dc.subject.asfaNecrosesen
dc.subject.asfaVirusesen


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