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dc.contributor.authorMaeno, Yukio
dc.contributor.authorde la Peña, Leobert D.
dc.contributor.authorCruz-Lacierda, Erlinda R.
dc.date.accessioned2014-05-20T05:47:59Z
dc.date.available2014-05-20T05:47:59Z
dc.date.issued2007
dc.identifier.citationMaeno, Y., de la Peña, L. D., & Cruz-Lacierda, E. R. (2007). Susceptibility of fish species cultured in mangrove brackish area to piscine nodavirus. Japan Agricultural Research Quarterly, 41(1), 95-99.en
dc.identifier.issn0021-3551
dc.identifier.urihttp://hdl.handle.net/10862/2065
dc.description.abstractSusceptibility of orange-spotted grouper Epinephelus coioides, Asian sea bass Lates calcarifer, mangrove red snapper Lutjanus argentimaculatus, milkfish Chanos chanos, and rabbitfish Siganus guttatus to piscine nodavirus from orange-spotted grouper was studied by experimental infection. The fish were intraperitoneally injected with 0.05 mL of the filtrate homogenate of infected organs from diseased grouper at 106.8, 105.8 or 104.8 TCID50/fish, while the control group received 0.05 mL of Hanks’ balanced salt solution. Clinical signs such as lethargy, anorexia and darkened pigmentation were observed in the orange-spotted grouper, Asian sea bass, mangrove red snapper, and milkfish injected with high and medium doses of the homogenate. Although no or little mortality occurred in the experimentallyinfected fish 10 days post-inoculation, viral nervous necrosis specific lesions such as severe necrosis and vacuolation in the brain and retina were produced in these four fish species. The virus was reisolated in SSN-1 cells inoculated with the filtrated tissue homogenate of survivors in all doses for all four fish species. However, in the experimentally infected rabbitfish no histological lesion was observed, and no virus was reisolated. These results indicate that grouper, sea bass, mangrove red snapper, and milkfish are susceptible to the piscine nodavirus isolated from diseased grouper.en
dc.language.isoenen
dc.publisherTropical Agricultural Research Centreen
dc.relation.urihttps://www.jstage.jst.go.jp/article/jarq/41/1/41_95/_pdf
dc.subjectChanos chanosen
dc.subjectLutjanus argentimaculatusen
dc.subjectSiganus guttatusen
dc.subjectLates calcariferen
dc.subjectEpinephelus coioidesen
dc.titleSusceptibility of fish species cultured in mangrove brackish area to piscine nodavirusen
dc.typeArticleen
dc.identifier.doi10.6090/jarq.41.95
dc.citation.volume41
dc.citation.issue1
dc.citation.spage95
dc.citation.epage99
dc.citation.journalTitleJapan Agricultural Research Quarterlyen
seafdecaqd.library.callnumberVF SJ 0883
seafdecaqd.databank.controlnumber2007-01
dc.subject.asfaInfectious diseasesen
dc.subject.asfaVirusesen
dc.subject.asfaMangrovesen
dc.subject.asfaFish cultureen


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