Immune responses of Asian sea bass, Lates calcarifer Bloch, against an inactivated betanodavirus vaccine
MetadataShow full item record
Cited times in Scopus
Asian sea bass, Lates calcarifer (Bloch), exhibited strong immune responses against a single injection of the formalin-inactivated red-spotted grouper nervous necrosis virus (RGNNV), a betanodavirus originally isolated in Japan. Fish produced neutralizing antibodies at high titre levels from days 10 (mean titre 1:480) to 116 (1:1280), with the highest titre at day 60 post-vaccination (1:4480). When fish were challenged with the homologous RGNNV at day 54 post-vaccination, there were no mortalities in both the vaccinated and unvaccinated control fish. However, a rapid clearance of the virus was observed in the brains and kidneys of vaccinated fish, followed by a significant increase in neutralizing-antibody titres. Furthermore, the vaccine-induced antibodies potently neutralized Philippine betanodavirus isolates (RGNNV) in a cross-neutralization assay. The present results indicate the potential of the formalin-inactivated RGNNV vaccine against viral nervous necrosis (VNN) of Asian seabass.
CitationPakingking Jr., R. V., Seron, R., de la Peña, L. D., Mori, K., Yamashita, H., & Nakai, T. (2009). Immune responses of Asian sea bass, Lates calcarifer Bloch, against an inactivated betanodavirus vaccine.
We would like to thank Dr. Gilda Lio-Po for giving valuable suggestions on this study. We also gratefully acknowledge Dr. Evelyn Grace de Jesus-Ayson and some staff of Finfish Hatchery and Biolab for their kind assistance during the experiment. This study was funded in part by the Government of Japan Trust Fund through the Regional Fish Disease Project (study code: 8001-T-FT-FH0507) and by a Grant-in-Aid for Scientific Research (No.18076) from the Ministry of Agriculture, Forestry and Fisheries of Japan.
- Journal Articles 
Showing items related by title, author, creator and subject.
Book chapterKGS Andrino-Felarca, EG Estante & CC Lazado - In CMA Caipang, MBI Bacano-Maningas & FF Fagutao (Eds.), Biotechnological Advances in Shrimp Health Management in the Philippines, 2015 - Research SignpostShrimp is a high-value commodity and one of the major aquaculture species in the world, including the Philippines. The shrimp farming industry is dominated by the black tiger shrimp Penaeus monodon and the Pacific white shrimp, Penaeus vannamei. Intensification in shrimp aquaculture to meet the global demand resulted to several socio-economic and biophysical production bottlenecks. Consequently, the issues besetting the industry had raised several questions on its sustainability. In particular, viral diseases remain a constant threat and a significant concern in many shrimp producing countries especially in the developing world. In this chapter, current knowledge on major viral pathogens affecting shrimp aquaculture in the Philippines is presented and discussed. The discussion is focused on white spot syndrome virus (WSSV), monodon baculovirus (MBV), infectious hypodermal and hematopoietic necrosis virus (IHHNV), hepatopancreatic parvovirus (HPV). yellow head virus (YHV), and taura syndrome virus (TSV). Updates on their clinical signs, transmission and distribution are presented. Records of incidence in the Philippines are provided as well. The second half of the chapter discusses some of the methods how to control viral diseases in shrimp farming with a particular focus on vaccination, biosecurity and diagnostics.
ArticleAE Polk, B Amsden, DJ Scarratt, A Gonzal, AO Okhamafe & MFA Goosen -
Aquacultural Engineering, 1994 - Aquacultural Engineering Society (AES)Potentially, the most useful method of fish vaccination is oral administration. However, this technique is presently only partially effective because of the apparent destruction of the vaccine in the fish digestive system, as well as interaction of the vaccine with the feed components. The authors' approach to this problem was to protect the vaccine by entrapping it within semi-permeable biocompatible microcapsules. Two bioactive agents — a vaccine, Vibrio bacterin and a model protein, bovine serum albumin (BSA) — were entrapped in chitosan-alginate microcapsules by extrusion of a sodium alginate/bioactive agent suspension into a CaCl2/chitosan solution. The results of experiments on the effects of varying the encapsulation parameters and the presence of enzymes on the in-vitro release of entrapped bioactive material are presented. This technique has been developed as a simple, quick, and inexpensive method for oral delivery. Capsules may be dried and mixed with feed for ease of administration, particularly to young fish which are susceptible to high mortality from both the disease and harsher vaccination procedures.
Susceptibility of hatchery-reared snubnose pompano Trachinotus blochii to natural betanodavirus infection and their immune responses to the inactivated causative virus Mass mortality of snubnose pompano Trachinotus blochii fry exhibiting dark coloration, anorexia, and abnormal swimming behavior was recently documented at the hatchery of the Aquaculture Department of the Southeast Asian Fisheries Development Center, Philippines. Samples of brain tissues were collected from affected fish and processed for RT-PCR amplification and virus isolation in cell culture. Infected E-11 cells exhibited cytopathic effect characteristic of betanodavirus. Histopathology of moribund fish showed pronounced vacuolations in the brain, spinal cord, and retina. An RT-PCR product of approximately 430 bp was amplified from the culture supernatant of betanodavirus-infected E-11 cells and sequenced. Sequencing of the T4 region of the coat protein gene (RNA 2) revealed clustering of the isolated virus within the red-spotted grouper nervous necrosis virus type. The pathogenicity of the isolated betanodavirus in healthy pompano juveniles and fry was determined via intramuscular injection and immersion challenges, respectively. Higher mortality rates were obtained in challenged fish compared with the controls. An inactivated vaccine was subsequently prepared by treating the clarified betanodavirus with formalin. Pompano juveniles intraperitoneally injected with the inactivated vaccine exhibited neutralizing antibodies from days 15 (mean titer 1:240) to 125 (1:560) with the highest titer noted at day 64 (1:2240) post-vaccination. Additionally, pompano fry bath-vaccinated and consequently bath-challenged with betanodavirus at day 35 post-vaccination showed higher survival rate compared with the control, indicating the potential of the inactivated betanodavirus vaccine against VNN in pompano fry and juveniles.