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dc.contributor.authorMaralit, Benedict A.
dc.contributor.authorVentolero, Minerva Fatimae H.
dc.contributor.authorManingas, Mary Beth B.
dc.contributor.authorAmar, Edgar C.
dc.contributor.authorSantos, Mudjekeewis D.
dc.date.accessioned2015-05-25T01:03:48Z
dc.date.available2015-05-25T01:03:48Z
dc.date.issued2014
dc.identifier.citationMaralit, B. A., Ventolero, M. F. H., Maningas, M. B. B., Amar, E. C., & Santos, M. D. (2014). Subtracted transcriptome profile of tiger shrimp (Penaeus monodon) that survived WSSV challenge. Dataset Papers in Science, 2014, Article ID 807806, 11 pages. https://doi.org/10.1155/2014/807806en
dc.identifier.urihttp://hdl.handle.net/10862/2249
dc.description.abstractThere is increased interest in the development of virus-resistant or improved shrimp stock because production is currently hindered by outbreaks and limited understanding of shrimp defense. Recent advancement now allows for high-throughput molecular studies on shrimp immunity. We used next-generation sequencing (NGS) coupled with suppression subtractive hybridization (SSH) to generate a transcriptome database of genes from tiger shrimp that survived White spot syndrome virus (WSSV) challenge. A total of 9,597 unique sequences were uploaded to NCBI Sequence Read Archive with accession number SRR577080. Sixty-five unique sequences, 6% of the total, were homologous to genes of Penaeus monodon. Genes that were initially related to bacterial infection and environmental stress such as 14-3-3 gene, heat shock protein 90, and calreticulin were also found including a few full-length gene sequences. Initial analysis of the expression of some genes was done. Hemocyanin, ferritin, and fortilin-binding protein exhibited differential expression between survivor and control tiger shrimps. Furthermore, candidate microsatellite markers for brood stock selection were mined and tested. Four trinucleotide and one dinucleotide microsatellites were successfully amplified. The study highlights the advantage of the NGS platform coupled with SSH in terms of gene discovery and marker generation.en
dc.language.isoenen
dc.publisherHindawi Publishing Corporationen
dc.relation.urihttp://downloads.hindawi.com/archive/2014/807806.pdf
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/
dc.titleSubtracted transcriptome profile of tiger shrimp (Penaeus monodon) that survived WSSV challengeen
dc.typeArticleen
dc.identifier.doi10.1155/2014/807806
dc.citation.volume2014
dc.citation.spageArticle ID 807806, 11 pages
dc.citation.journalTitleDataset Papers in Scienceen
dc.subject.asfagenesen
dc.subject.asfadatabasesen
dc.subject.asfaproteinsen
dc.subject.asfahybridizationen
dc.subject.asfamicrosatellitesen
dc.subject.asfalengthen
dc.subject.asfagenetic markersen
dc.subject.asfageneticsen
dc.subject.asfanucleotide sequenceen
dc.subject.asfaDNAen
dc.identifier.essn2314-8497


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    These papers were contributed by Department staff to various national and international journals.

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