Biomass production, proximate composition and fatty acid profile of the local marine thraustochytrid isolate, Schizochytrium sp. LEY7 using low-cost substrates at optimum culture conditions
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This study was conducted to investigate low-cost substrate alternative and the optimum culture conditions in mass producing the local marine thraustochytrid, Schizochytrium sp. LEY7 isolated from the mangrove leaves of Baybay, Southern Leyte Philippines. Results showed that Schizochytrium sp. LEY7 is able to utilize commercial grade glucose and yeast extract from NaCl-treated baker's yeast as source of carbon and micronutrients respectively. Cost of mass producing the thraustochytrid isolate using the alternative production substrates was substantially reduced. Incubation temperature and salinity levels were the two growth factors significantly affecting the biomass production of the isolate. The short duration of lag phase shown by the isolate suggests a growth advantage in that cells are readily able to adapt to their new environment. Total lipids averaged to 19.4%. Principal fatty acids were palmitic acid (C16:0) with 33.52% and docosahexanoic acid (DHA, C22:6n-3) with 39.92% proportion to total fatty acid. Eicosapentaenoic (C20:5n-3, 1.01%) and arachidonic (C20:4n-3, 0.90%) are present but in relatively lower amount. Our findings showed the potential of low-cost substrate in mass producing the local thraustochytrid isolate, Schizochytrium sp. LEY7 as lipid and polyunsaturated fatty acid source in aquaculture. Biomass production was enhanced by optimizing the culture conditions.
CitationLudevese-Pascual, G., Dela Peña, M., & Tornalejo, J. (2016). Biomass production, proximate composition and fatty acid profile of the local marine thraustochytrid isolate, Schizochytrium sp. LEY7 using low-cost substrates at optimum culture conditions.
PublisherJohn Wiley and Sons
This study was funded by the Aquaculture Department, Southeast Asian Fisheries Development Center (SEAFDEC/AQD) with the study code Nr-03-Y2010T.
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Adsorption and biomass concentration of thraustochytrid Schizochytrium aggregatum (Goldstein and Belsky) in Bunker C Oil BGS Sarinas, LD Gellada, MLT Torrigue, DN Sibonga, ES Torrato, JG Malagad, JG Feril, LAJ Bondoc, JCA Roncal & JA Tornalejo -
Journal of Environmental Science and Management, 2014 - School of Environmental Science and Management, University of the Philippines Los BañosDiverse array of microorganisms such as bacteria, fungi and protists are involved during oil spill. Each microorganism has its own specific function whether it has to degrade or adsorb hydrocarbons. One important microorganism is the Thraustochytrid that is a fungoid protist and are common in marine and estuarine habitats. Numerous studies existed on the biodegradation and adsorption of Thraustochytrids on various substances but not on Bunker C oil. Thus, this study aimed to determine the adsorption capacity and mean biomass of Thraustochytrids in Bunker C oil using different cell densities measured in grams. All of the three treatments or cell densities (1 x 105 cells ml-1, 1 x 106 cells ml-1 and 1 x 107 cells ml-1) were triplicated and average values were recorded. Oil dispersant was used as a control. It showed that Thraustochytrid with 1 x 107 cells ml-1 showed the highest adsorbed oil (.057 ḡ) among the three cell densities and showed significant difference at p = .01 but comparable to the control (.066 ḡ). In terms of biomass concentration, all cell densities showed no significant difference at p = .01. Thraustochytrid is a promising tool during oil spill because it has the capacity to adsorb oil.
Use of thraustochytrid Schizochytrium sp. as source of lipid and fatty acid in a formulated diet for abalone Haliotis asinina (Linnaeus) juveniles MR de la Peña, MB Teruel, JM Oclarit, MJA Amar & EGT Ledesma -
Aquaculture International, 2016 - Springer VerlagThe effects of using thraustochytrid Schizochytrium sp. as source of lipid and fatty acids in a formulated diet on growth, survival, body composition, and salinity tolerance of juvenile donkey’s ear abalone, Haliotis asinina, were investigated. Treatments consisted of diets either containing a 1:1 ratio of cod liver oil (CLO) and soybean oil (SBO) (Diet 1) or thraustochytrid (Diet 2) as source of lipid and fatty acids at 2 % level. Natural diet Gracilariopsis heteroclada (Diet 3) served as the control. No significant difference in growth was observed in abalone fed Diet 3 (SGR: 5.3 % BW day−1; DISL: 265 μm day−1) and Diet 2 (SGR: 5.2 % BW day−1; DISL: 255 μm day−1). Survival ranged from 78 to 85 % for all treatments and was not significantly different from each other. A 96-h salinity stress test showed highest survival of 84 % in abalone fed Diet 2 compared with those fed diets 1 and 3 (42 %). The high growth rate of abalone fed Diet 2 and high tolerance to low salinity could be attributed to its high DHA content (8.9 %), which resulted to its high DHA/EPA ratio of 10.5 %. These fatty acids play a significant role in abalone nutrition. The fatty acid profile of abalone meat is a reflective of the fatty acid profile of the oil sources in the diet. The present study suggests that the use of Schizochytrium oil in lieu of CLO and SBO can support good growth of abalone which is comparable with abalone fed the natural seaweeds diet.
Low-cost production of the marine thraustochytrid isolate, Schizochytrium sp. LEY7 as larval live feed enrichement for the mangrove snapper, Lutjanus sp. G Ludevese-Pascual, M de la Peña, O Reyes & J Tornalejo - In CI Hendry (Ed.), Larvi ’13 – Fish & Shellfish Larviculture Symposium, 2013 - Laboratory of Aquaculture & Artemia Reference Center, Ghent UniversityThis study demonstrates the potential of low-cost production substrates in mass producing the Schizochytrium sp. LEY7 for maximum DHA production. High biomass yield was achieved by optimizing the culture conditions particularly the incubation temperature and salinity levels. Preliminary data on feeding trials have shown promising results and therefore confirmatory experiments need to be conducted to verify the results obtained.