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dc.contributor.authorMamauag, Roger Edward P.
dc.contributor.authorRagaza, Janice A.
dc.date.accessioned2016-10-06T01:40:33Z
dc.date.available2016-10-06T01:40:33Z
dc.date.issued2017
dc.identifier.citationMamauag, R. E. P., & Ragaza, J. A. (2017). Growth and feed performance, digestibility and acute stress response of juvenile grouper (Epinephelus fuscoguttatus) fed diets with hydrolysate from milkfish offal. Aquaculture Research, 48(4), 1638-1647en
dc.identifier.issn1355-557X
dc.identifier.urihttp://hdl.handle.net/10862/3075
dc.description.abstractNutritional qualities of fish processing by-products can further be improved through enzymatic hydrolysis. The objective of this study was to elucidate the efficacy of hydrolysed milkfish offal at different inclusion levels when fed to juvenile grouper, Epinephelus fuscoguttatus, with an initial body weight of 2.88 ± 0.06 g. The animals were fed for 56 days with seven diets supplemented with 0 (control), 5%, 15% and 25% of milkfish offal (MO) and milkfish offal hydrolysate (MOH). The diets were formulated to be isonitrogenous (45%) and isolipidic (11%). The diets were assigned to 21 tanks (15 fish per tank) with each diet having three replicates. Results from the experimental trials indicated that feed conversion efficiency, feed intake and weight gain of fish significantly (P < 0.05) improved when fed diets with MOH. No significant differences within the rest of the dietary treatments were observed. Survival rate (>90%) did not differ in all the dietary treatments. Proximate composition (crude protein, crude fat and ash) indicated no significant difference among fish fed from all the dietary treatments. Apparent digestibility of MOH indicated a 95% and 66% digestibility for protein and dry matter respectively. Plasma stress parameters (cortisol and glucose) were not influenced by the dietary treatment when fish were subjected to an acute stressor (5-min chasing). Liver morphology indicated normal hepatocyte shape and the presence of lipid droplets in fish fed from all the dietary treatments. The results indicated that milkfish offal processed as hydrolysate can be utilized in grouper diets and can promote growth and feed efficiency when supplied at 10–15%.en
dc.language.isoenen
dc.publisherWileyen
dc.subjectMilkfish offalen
dc.subjectEpinephelus fuscoguttatus
dc.subjectHydrolysateen
dc.subjectStress responseen
dc.titleGrowth and feed performance, digestibility and acute stress response of juvenile grouper (Epinephelus fuscoguttatus) fed diets with hydrolysate from milkfish offalen
dc.typeArticleen
dc.identifier.doi10.1111/are.12999
dc.citation.volume48
dc.citation.issue4
dc.citation.spage1638
dc.citation.epage1647
dc.citation.journalTitleAquaculture Researchen
dc.subject.asfaDigestibilityen
dc.identifier.essn1365-2109


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