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dc.contributor.authorSamentar, Lorena P.
dc.contributor.authorAyson, Felix G.
dc.contributor.authorde Jesus-Ayson, Evelyn Grace T.
dc.contributor.authorFormacion, Minda J.
dc.identifier.citationSamentar, L. P., Ayson, F. G., de Jesus-Ayson, E. G. T., & Formacion, M. J. (2013). Cloning of mangrove red snapper (Lutjanus argentimaculatus) growth hormone cDNA and mRNA expression during early development. Philippine Journal of Natural Sciences, 18(2), 21-29.en
dc.description.abstractGrowth hormone regulates growth and development in vertebrates. As a first step to understand the role of growth hormone in the regulation of growth and development of the mangrove red snapper Lutjanus argentimaculatus, the red snapper growth hormone (sGH) cDNA was cloned using reverse transcription - polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends. The expression of sGH mRNA in embryos and larvae was examined also by RT-PCR. Excluding the poly-A tail, the full-length red snapper GH cDNA is 945 base pairs (bp) long. It contains untranslated regions of 99 bp and 234 bp in the 5’ and 3’ ends, respectively. It has an open reading frame of 612 bp coding for a signal peptide of 17 amino acids and a mature hormone of 187 amino acid residues. Red snapper GH contains 4 cysteine residues and the typical polyadenylation site 16 bp upstream of the poly-A tail. Based on the amino acid sequence of the mature hormone, sGH shows higher sequence identity (>75%) to GHs of perciforms like grouper, seabass, tilapia and rabbitfish than to GHs of salmonids and carps. Semi-quantitative RT-PCR showed that expression of sGH mRNA commenced two days after hatching.en
dc.publisherUniversity of the Philippines Visayasen
dc.subjectLutjanus argentimaculatusen
dc.titleCloning of mangrove red snapper (Lutjanus argentimaculatus) growth hormone cDNA and mRNA expression during early developmenten
dc.citation.journalTitlePhilippine Journal of Natural Sciencesen
dc.subject.asfalarval stageen
dc.subject.asfapolymerase chain reactionen
dc.subject.asfaamino acid sequencesen

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  • Journal Articles [1166]
    These papers were contributed by Department staff to various national and international journals.

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