Nutritional evaluation of distiller's dried grain with soluble as replacement to soybean meal in diets of milkfish, Chanos chanos and its effect on fish performance and intestinal morphology
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A 90-day feeding trial was conducted on milkfish, Chanos chanos with an initial mean body weight of 3.07 ± 0.17 g (mean ± standard error of mean). Six treatment diets were formulated to contain 0 g/kg (Diet 1), 150 g/kg (Diet 2), 25 g/kg (Diet 3), 300 g/kg (Diet 4), 350 g/kg (Diet 5) and 450 g/kg (Diet 6) distiller's dried grain with soluble (DDGS). All the dietary treatments were isonitrogenous (350 g/kg crude protein) and isolipidic (6% crude lipid). Result of the feeding trial indicated that growth rates, feed intake and feed efficiency were not significantly (p > .05) affected by inclusion levels of DDGS by up to 450 g/kg in the feed. Proximate body composition (crude protein, crude lipid, ash, fibre) in fish fed the dietary treatments were not significantly (p > .05) affected as well. The DDGS when used as a milkfish ingredient has a protein digestibility of 910 g/kg, fat disgetsibility of 850 g/kg, carbohydrate digestibility of 750 g/kg and a dry matter digestibility of 520 g/kg Results from the intestinal morphology displayed no apparent pathological changes in the digestive tract of fish fed all dietary treatments. These results indicate that DDGS can be efficiently utilized by milkfish by up to 450 g/kg without negatively affecting performance parameters and intestinal morphology.
Mamauag, R. E. P., Ragaza, J. A., & Nacionales, T. J. (2017). Nutritional evaluation of distiller’s dried grain with soluble as replacement to soybean meal in diets of milkfish, Chanos chanos and its effect on fish performance and intestinal morphology. Aquaculture Nutrition. https://doi.org/10.1111/anu.12470
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Conference paperLV Benitez - In RD Fortes, LC Darvin & DL de Guzman (Eds.), Fish and crustacean feeds and nutrition : Proceedings of the seminar-workshop on fish and crustacean feeds and nutrition held on 25-26 February 1985 at UPV, Iloilo City, 1989 - Philippine Council for Aquatic and Marine Research and DevelopmentThis paper reviews recent work on milkfish nutrition. Substantial progress had been made towards understanding the digestive physiology of milkfish. Major enzaymes envolved in the digestions of carbohydrates, protein and lipids had been detected in the pyloric caece, intestines and pancreas of milkfish. The most active carbohydrates were involved in the hydrolysis of α - glocosidic bonds. Intestinal amylase activity consistently reached the peak at about noon when milkfish gut was full. This confirms that milkfish is s daytime feeder. No cellulase activity was detected in any region orf the digertive treat although the fish relies heavily algae and other plant source for food. Trypsin, chymotrypsin and general proteases were also detected in milkfish digestive tract. A powerful milkfish trypsin inhabitor was detected in the filementous algae, Chaetomorpha brachygona which is predominant species in lumot. Lipass in the pancreas and intestines had two pH optima, suggesting a physiologic versatility for lipid digestion in milkfish. There is a limit information on the nutrient requirement of milkfish. Most studies showed that milkfish fry has a dietary requirement of 40% protein, and 7-10 lipid. Studies on the protein-energy requirement of fingerlings suggested that 30-40% protein, 10% fat and 25% carbohydrates are required. Subsequent studies showed an optimum protein energy to total metabolizable energy ratio of 44.4%. Amino acid test diets for milkfish had been formulated to contain white fish meal, gelatin and approprate amino acid mix.
Lactate dehydrogenase isozyme patterns during the development of milkfish, (Chanos chanos (Forskal)) PD Requintina, LM Engle & LV Benitez -
Kalikasan, The Philippine Journal of Biology, 1981 - University of the Philippines at Los BañosPolyacrylamide disc gel electrophoresis was done to determine the lactate dehydrogenase (LDH) isozyme patterns for fry (5-3 mg), fingerling (6-12 g), pond-size (150-250 g) and adult (6-9 kg) milkfish. The patterns were tissue specific; the different tissues examined, viz., eye, liver, heart, and skeletal muscle had different expressions of LDH isozymes. The resolved patterns appeared to be products of LDH gene loci A, B, and C. Subunits A and B were present in all tissues. A4 and B4 were predominant in skeletal and heart muscle, respectively; the two associated non-randomly in vivo and formed only the heteropolymers A3B and AB3. A liver band, L4, was most conspicuous in the fingerling, pond-size, and adult; it was assumed to be coded by locus C. A negatively charged band, X4, was detected in fully developed ovary and in fry homogenized as whole individuals, but it could not be resolved in tissues of fingerling. Six-mo old stunts and 3-mo old fingerlings had similar LDH patterns for all tissues examined. The patterns for 11-mo old stunts and fingerlings also were similar but the one for the eye of the former was the same pattern resolved for the eye of adults. There was no change in the LDH isozyme patterns of milk fish stunted for 6 mo under different salinity levels (0-5, 15-20, 32-35 ppt).
Conference paperCL Marte, J Toledo, G Quinitio & A Castillo - In JL Maclean, LB Dizon & LV Hosillos (Eds.), The First Asian Fisheries Forum. Proceedings of the First Asian Fisheries Forum, 26-31 May 1986, Manila, Philippines, 1986 - Asian Fisheries SocietyNatural spawnings of milkfish from floating cages were obtained from different stocks of 5-7 year-old milkfish in 1980, 1981, 1983 and 1985. The maximum number of eggs collected in 1980, using a series of 1-m diameter stationary plankton nets, was about 900. Increased collections were obtained in succeeding years with different types of egg collectors and methods. Egg collection, however, is inferior when based on the expected number of eggs spawned by a single female. Although no systematic study was attempted to compare efficiency of various egg-collecting gears and methods, the problems associated with the use of each gear are presented. The experience may be used as a guide in future designs of efficient gears of collecting naturally-spawned eggs of milkfish or other fish species in floating cages.