Use of agar-bound microparticulate diet as alternative food for tropical abalone, Haliotis asinina (Linnaeus 1758) post-larvae in large-scale cultures
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The efficacy of using agar-bound microparticulate diet (A-MPD) as alternative food for abalone Haliotis asinina Linne post-larvae in large-scale culture was investigated. Larvae sourced from the hatchery-bred (HB) and wild-sourced (WS) broodstock were fed with either diatoms (TMT1-NF), agar-bound microparticulate diet (TMT2-A-MPD), or a combination of both feeds (TMT3-NF + A-MPD) in six 2-m3 tanks replicated over time. Three hundred thousand veliger larvae were stocked/tank containing 80 corrugated plates with mucus trails hanging on bamboo poles. Feeds were given at 0900 h starting at day 3 with seawater flow through introduced every 1400 h starting day 5. Two-way analysis of variance determined significant differences (p < 0.05) in survival and shell length between larval sources and feed types. Tukey’s post hoc test established differences among treatment means. At day 30, survival for HB- and WS-sourced larvae was significantly higher (42%) in TMT3 compared with TMT2 having 35% for HB and 38% for WS (p < 0.05). Larvae fed with TMT1 had significantly lowest survival among the three treatments. Survival at 60 and 90 days did not show significant difference for TMT2 and TMT3 regardless of broodstock source. Post-larval shell growth (90 days), from both sources fed with TMT2 and TMT3, was significantly higher than TMT1 (p < 0.05). Larval performance did not show any significant interactions between HB and WS broodstock. The use of A-MPD alone or in combination may elicit improvement in survival and shell length growth in abalone larvae regardless of larval sources. A-MPD may be used as full or partial replacements to diatoms as alternative food for abalone post-larvae in large-scale culture.
CitationBautista-Teruel, M. N., Maquirang, J. R. H., de la Peña, M. R., & Balinas, V. T. (2017). Use of agar-bound microparticulate diet as alternative food for tropical abalone, Haliotis asinina (Linnaeus 1758) post-larvae in large-scale cultures.
The authors would like to thank SEAFDEC/AQD (study code 5309-TTV-MO-312T) through its chief Dr. Felix Ayson and DOST for the funds provided; the SEAFDEC/AQD Abalone hatchery staff: Mr. Rafael Barrido, Mr. Ramil Piloton, and Mr. Nestor Bayona for their valuable suggestions in carrying out post-larval rearing work; Ms. Rena Santizo and Ms. Analyn Asutilla for their significant help and contribution in the study; and Mr. Richard Tantiado for the technical assistance in the conduct of experiment.
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Proceedings of the National Seaweed Planning Workshop held on August 2-3, 2001, SEAFDEC Aquaculture Department, Tigbauan, Iloilo. AQ Hurtado, NG Guanzon Jr., TR de Castro-Mallare & MRJ Luhan (Eds.) - 2002 - Aquaculture Department, Southeast Asian Fisheries Development CenterSeaweed forming is one of the major livelihoods among coastal communities in the Philippines, particularly to some 180,000 families in the Sulu Archipelago. In 1999, the Philippines exported more than 35,000 tons of dried seaweeds (US$ 44M) making the country the 4th largest producer of seaweeds and 8th largest producer of carrageenan in the world. However, improper post-harvest management (i.e. cleaning; drying by salting or steaming; adulteration of seaweeds with sand, dust, and dirt for added weight; storage; and baling) reduces quality, which eventually dictates the market price. The National Seaweed Planning Workshop was organized by a collaborative effort of SEAFDEC Aquaculture Department (AQD) and the Bureau of Fisheries and Aquatic Resources (BFAR) for the formulation of a Code of Practice for the Seaweed Industry in order to minimize industry malpractices and to sustain its position in the international market. The National Seaweed Planning Workshop invited several seaweed industry associations, and representatives from the government, NGOs and academic and research organizations conducting seaweed research and development to discuss the research and development programs of the different participating agencies, identify and validate problems and concerns of the seaweed industry, and agree on strategies of solving problems in seaweed farming like disease management, post-harvest facilities and research funding. This proceedings documents the National Seaweed Planning Workshop. Hopefully, the contributions would help in the drafting of the Code of Practice in attaining a sustainable seaweed industry.
Book chapterL Ruangpan - In Laboratory manual of standardized methods for antimicrobial sensitivity tests for bacteria isolated from aquatic animals and environment, 2004 - Aquaculture Department, Southeast Asian Fisheries Development CenterThe agar dilution technique is used to measure qualitatively the in vitro activity of an antimicrobial agent against the test bacteria. In this method, graded amounts of antibiotics are incorporated in agar plates and inoculated in spots with the organisms under study. If the organism under study is susceptible to the incorporated antibiotic, no bacterial growth is expected in agar plates with higher amounts of the drugs. Bacterial growth is observed as the antibiotic concentration in the agar plate diminishes. Inhibition of growth at the minimum or lowest concentration of antibiotic is regarded as the end point.
Conference paperAQ Hurtado-Ponce - In F Lacanilao, RM Coloso & GF Quinitio (Eds.), Seminar-Workshop on Aquaculture Development in Southeast Asia and Prospects for Seafarming and Searanching; 19-23 August 1991; Iloilo City, Philippines., 1994 - Aquaculture Department, Southeast Asian Fisheries Development CenterThis paper reviews the studies on Gracilaria/Gracilariopsis conducted from 1988 to 1991 by the Aquaculture Department, Southeast Asian Fisheries Development Center. It includes 114 species of macrobenthic algae collected in Panay, the nomenclature of Gracilariopsis heteroclada previously described as Gracilaria sp., and the biology, ecology, and farming systems of Gracilariopsis. Agar quality of the different species of Gracilaria and the effect of seasonal variation on the quality and quantity of agar produced from Gracilariopsis heteroclada were also studied.