Use of agar-bound microparticulate diet as alternative food for tropical abalone, Haliotis asinina (Linnaeus 1758) post-larvae in large-scale cultures
Abstract
The efficacy of using agar-bound microparticulate diet (A-MPD) as alternative food for abalone Haliotis asinina Linne post-larvae in large-scale culture was investigated. Larvae sourced from the hatchery-bred (HB) and wild-sourced (WS) broodstock were fed with either diatoms (TMT1-NF), agar-bound microparticulate diet (TMT2-A-MPD), or a combination of both feeds (TMT3-NF + A-MPD) in six 2-m3 tanks replicated over time. Three hundred thousand veliger larvae were stocked/tank containing 80 corrugated plates with mucus trails hanging on bamboo poles. Feeds were given at 0900 h starting at day 3 with seawater flow through introduced every 1400 h starting day 5. Two-way analysis of variance determined significant differences (p < 0.05) in survival and shell length between larval sources and feed types. Tukey’s post hoc test established differences among treatment means. At day 30, survival for HB- and WS-sourced larvae was significantly higher (42%) in TMT3 compared with TMT2 having 35% for HB and 38% for WS (p < 0.05). Larvae fed with TMT1 had significantly lowest survival among the three treatments. Survival at 60 and 90 days did not show significant difference for TMT2 and TMT3 regardless of broodstock source. Post-larval shell growth (90 days), from both sources fed with TMT2 and TMT3, was significantly higher than TMT1 (p < 0.05). Larval performance did not show any significant interactions between HB and WS broodstock. The use of A-MPD alone or in combination may elicit improvement in survival and shell length growth in abalone larvae regardless of larval sources. A-MPD may be used as full or partial replacements to diatoms as alternative food for abalone post-larvae in large-scale culture.
Citation
Bautista-Teruel, M. N., Maquirang, J. R. H., de la Peña, M. R., & Balinas, V. T. (2017). Use of agar-bound microparticulate diet as alternative food for tropical abalone, Haliotis asinina (Linnaeus 1758) post-larvae in large-scale cultures.Publisher
Springer VerlagAcknowledgment/Sponsorship
The authors would like to thank SEAFDEC/AQD (study code 5309-TTV-MO-312T) through its chief Dr. Felix Ayson and DOST for the funds provided; the SEAFDEC/AQD Abalone hatchery staff: Mr. Rafael Barrido, Mr. Ramil Piloton, and Mr. Nestor Bayona for their valuable suggestions in carrying out post-larval rearing work; Ms. Rena Santizo and Ms. Analyn Asutilla for their significant help and contribution in the study; and Mr. Richard Tantiado for the technical assistance in the conduct of experiment.
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Article
Evaluation of agar-bound microparticulate diet as alternative food in abalone hatchery: Effects of agar concentrations and feeding frequencies
-Journal of Shellfish Research , 2013 - National Shellfisheries AssociationThe performance of an agar-bound microparticulate diet (A-MPD) was evaluated on feeding postlarval abalone Haliotis asinina, focusing on the effects of agar concentrations and feeding frequencies. Larval abalone, obtained from the Southeast Asian Fisheries Development Center, Aquaculture Department hatchery, were reared in 60-L flow-through tanks with UV-filtered seawater. They were fed 1,200 mg A-MPD bound with either 5.0 mg/mL agar solution, 7.5 mg/mL agar solution, 10.0 mg/mL agar solution, and 12.5 mg/mL agar solution, or a natural diet consisting of diatoms at different feeding frequencies (daily, every other day, or every 2 d) starting at day 5. A 5 × 3 factorial experiment in a completely randomized design tested the effects of various treatments on postlarval settlement and survival after days 15 and 90. Scheffé's postcomparison test determined differences among treatments means. Postlarval settlement and survival were not significantly different in diets bound with higher agar concentrations and tested in 3 feeding frequencies. At lower levels of agar incorporation in diets, however, settlement and survival counts became significantly higher on daily feeding. Postlarval settlement and survival were significantly highest with abalone fed a diet bound with 7.5 mg/mL agar solution on a daily feeding frequency. Average percent weight loss in the feed was higher with lower levels of agar incorporation. Average particle size of both A-MPD and diatoms was 4–5 µm. Crude protein content of A-MPD was 42.7%; that of diatoms was 14.9%. A-MPD may be used as alternative food in abalone hatcheries with the incorporation of 7.5 mg/mL agar solution fed daily to abalone. -
Book | Conference publication
Proceedings of the National Seaweed Planning Workshop held on August 2-3, 2001, SEAFDEC Aquaculture Department, Tigbauan, Iloilo.
- 2002 - SEAFDEC Aquaculture DepartmentSeaweed forming is one of the major livelihoods among coastal communities in the Philippines, particularly to some 180,000 families in the Sulu Archipelago. In 1999, the Philippines exported more than 35,000 tons of dried seaweeds (US$ 44M) making the country the 4th largest producer of seaweeds and 8th largest producer of carrageenan in the world. However, improper post-harvest management (i.e. cleaning; drying by salting or steaming; adulteration of seaweeds with sand, dust, and dirt for added weight; storage; and baling) reduces quality, which eventually dictates the market price. The National Seaweed Planning Workshop was organized by a collaborative effort of SEAFDEC Aquaculture Department (AQD) and the Bureau of Fisheries and Aquatic Resources (BFAR) for the formulation of a Code of Practice for the Seaweed Industry in order to minimize industry malpractices and to sustain its position in the international market. The National Seaweed Planning Workshop invited several seaweed industry associations, and representatives from the government, NGOs and academic and research organizations conducting seaweed research and development to discuss the research and development programs of the different participating agencies, identify and validate problems and concerns of the seaweed industry, and agree on strategies of solving problems in seaweed farming like disease management, post-harvest facilities and research funding. This proceedings documents the National Seaweed Planning Workshop. Hopefully, the contributions would help in the drafting of the Code of Practice in attaining a sustainable seaweed industry. -
Conference paper
Utilization of seaweed resources
- In IJ Dogma Jr., GC Trono Jr. & RA Tabbada (Eds.), Culture and use of algae in Southeast Asia. Proceedings of the Symposium on Culture and Utilization of Algae in Southeast Asia, 8-11 December 1981, Tigbauan, Iloilo, Philippines, 1990 - Aquaculture Department, Southeast Asian Fisheries Development CenterA brief discussion is presented on the commercial importance of seaweeds in the Philippines, which is mainly concerned with their use as sources of industrial gums such as agar, carrageenan, and alginic acid. Carrageenan as a substitute for microbiological agar and the use of seaweeds as a binder of heavy metal pollutants are examined.
