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dc.contributor.authorMandal, Anup
dc.contributor.authorVarkey, Mathews
dc.contributor.authorSobhanan, Sobha P.
dc.contributor.authorMani, Anjali K.
dc.contributor.authorThampi Sam Raj, Yohannan C.
dc.contributor.editorQuinitio, Emilia T.
dc.contributor.editorEstepa, Fe Dolores P.
dc.contributor.editorThampi Sam Raj, Yohannan C.
dc.contributor.editorMandal, Anup
dc.date.accessioned2017-09-04T10:09:13Z
dc.date.accessioned2017-09-04T10:40:50Z
dc.date.accessioned2017-09-05T08:47:23Z
dc.date.available2017-09-04T10:09:13Z
dc.date.available2017-09-04T10:40:50Z
dc.date.available2017-09-05T08:47:23Z
dc.date.issued2015
dc.identifier.citationMandal, A., Varkey, M., Sobhanan, S. P., Mani, A. K., & Thampi Sam Raj, Y. C. (2015). Molecular genetic approaches to resolve taxonomical ambiguity of mud crab species (Genus Scylla) in Indian waters. In E. T. Quinitio, F. D. Parado-Estepa, Y. C. Thampi Sam Raj, & A. Mandal (Eds.), Proceedings of the International Seminar-Workshop on Mud Crab Aquaculture and Fisheries Management, 10-12 April 2013, Tamil Nadu, India (pp. 45-58). Tamil Nadu, India: Rajiv Gandhi Centre for Aquaculture (MPEDA).en
dc.identifier.isbn9788192989815
dc.identifier.urihttp://hdl.handle.net/10862/3209
dc.description.abstractMud crab or mangrove crab (Genus Scylla de Haan) is one of the most extensively cultured and economically important brachyuran crabs from the family Portunidae in Indo-Pacific countries, including India. Mud crabs exhibit variations in colour, size, spination, polygonal pattern and habitat. These contribute to the confusion in their identification. Accurate identification of the species is essential in the breeding programmes of domesticated stocks and is a crucial factor in the success of stock enhancement programmes. The taxonomic uncertainty of the genus Scylla in India is still an issue and several papers are being published using misleading identification. This is the first attempt to resolve the taxonomical ambiguity of mud crabs commonly found in Indian waters using multiple molecular genetic approaches. ITS-1, RAPD, PCRRFLP and mt-DNA sequencing along with traditional morphometric methods were used. Furthermore, a PCR method was developed by which mud crab species in India could be identified rapidly and accurately. The results of gene sequencing along with other molecular markers clearly indicated that the ‘green’ morph of Indian mud crab is S. serrata, while the ‘brown’ one is S. olivacea which was validated using the revised classification of mud crabs by Keenan et al. (1998). The S. serrata commonly mentioned in published literatures from India is S. olivacea and the S. tranquebarica, as believed by many Indian researchers, should be classified as S. serrata. Therefore, caution should be taken while interpreting or implementing the biological, molecular and aquaculture data published in those literatures.en
dc.language.isoenen
dc.publisherRajiv Gandhi Centre for Aquaculture (MPEDA)en
dc.subjectScylla serrataen
dc.subjectScylla olivaceaen
dc.subjectTaxonomic ambiguityen
dc.subjectmt-DNA sequencingen
dc.titleMolecular genetic approaches to resolve taxonomical ambiguity of mud crab species (Genus Scylla) in Indian watersen
dc.typeConference paperen
dc.citation.spage45
dc.citation.epage58
dc.citation.conferenceTitleProceedings of the International Seminar-Workshop on Mud Crab Aquaculture and Fisheries Management, 10-12 April 2013, Tamil Nadu, Indiaen
dc.subject.asfaidentification keysen
dc.subject.asfataxonomyen
dc.subject.asfapolymerase chain reactionen
dc.subject.asfaidentificationen
dc.subject.asfagenetic markersen
dc.subject.asfamtDNAen
dc.subject.asfaPCRen
dc.subject.asfamolecular markersen
dc.subject.scientificNameScylla serrataen


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