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dc.contributor.authorPerez, Karen Camille
dc.contributor.authorHuervana, Joana Joy
dc.contributor.authorEguia, Maria Rowena
dc.contributor.authorAblan-Lagman, Ma. Carmen
dc.date.accessioned2019-03-06T01:23:04Z
dc.date.available2019-03-06T01:23:04Z
dc.date.issued2017
dc.identifier.citationPerez, K. C., Huervana, J. J., Eguia, M. R., & Ablan-Lagman, M. C. (2017). Expressions of molt-inhibiting hormone (MIH) and extracellular signal-regulated kinase (ERK) during the molting stages of the giant mud crab, Scylla serrata. In Proceedings of the DLSU Research Congress 2017, De La Salle University, Manila, Philippines, June 20-22, 2017. Manila, Philippines: De La Salle University.en
dc.identifier.urihttp://hdl.handle.net/10862/3435
dc.description.abstractSurvival and growth of mud crabs and any other crustaceans depend on molting. Molting is influenced by several hormones, two of which are Molt-Inhibiting Hormone {MIH} and a molt promoting hormone, the Extracellular Signal-regulated Kinase (ERK). Controlling and synchronizing the molting of the crabs would be a big boost to the mud crab industry, especially in the production of soft shell crabs. The best way to control molting would be to stock juvenile crabs of the same molting stage together. In this study, hormone expressions were analyzed using quantitative Real-Time Polymerase Chain Reaction (1RT-PCR). Molt stages of 36 juvenile S. serrata were established based on MIH and ERK expression levels that was analyzed using Principal Component Analysis (PCA). One group of six individuals had MIH value equal to zero and presumed to be in the PR stage. The rest of the crabs had values for MIH and were presumed to be in the PM-IM stage. The value of ERK for the PM-IM stage was higher at 24.79 than in the PR stage at 21.67. Based on the data gathered, morphological markers may now be identified to standardize hormone expression. This would be the first study concerning the comparison of hormones during molting.en
dc.description.sponsorshipThis research was supported by the Department of Science and Technology - Philippine Council for Agriculture, Aquatic, and Natural Resources Research and Development and the Southeast Asian Fisheries Development Center - Aquaculture Department, along with CDO Foodsphere, Inc. for providing the mud crabs.en
dc.language.isoenen
dc.publisherDe La Salle Universityen
dc.relation.urihttps://www.dlsu.edu.ph/wp-content/uploads/pdf/conferences/research-congress-proceedings/2017/FNH/FNH-II-018.pdfen
dc.subjectScylla serrata
dc.subjectMoulting hormonesen
dc.subjectSoft shell crabsen
dc.titleExpressions of molt-inhibiting hormone (MIH) and extracellular signal-regulated kinase (ERK) during the molting stages of the giant mud crab, Scylla serrataen
dc.typeConference paperen
dc.citation.conferenceTitleProceedings of the DLSU Research Congress 2017, De La Salle University, Manila, Philippines, June 20-22, 2017en
dc.subject.asfaMoultingen
dc.subject.asfaEcdysonsen


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