Artificial substratum consisting of poly-β-hydroxybutyrate-based biodegradable plastic improved the survival and overall performance of postlarval tiger shrimp Penaeus monodon
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The use of artificial substratum consisting of poly‐β‐hydroxybutyrate (PHB)‐based biodegradable plastic for penaeid shrimp culture was investigated in the present study. The survival of postlarval tiger shrimp Penaeus monodon (30 ± 5 mg) provided with PHB substratum made out of PHB type DP9002 (Metabolix GmbH, Köln, Germany) was 88.7 ± 3.4% and this was significantly higher as compared to postlarvae provided conventional substratum consisting of polyvinylchloride (PVC) pipes (67.3 ± 6.5%). However, no significant weight improvement was observed for the postlarval tiger shrimp indicating that PHB could not be used as growth promoter. Nevertheless, a trend of improved robustness against adverse environmental conditions (lethal ammonium chloride concentration) and increased resistance to pathogenic Vibrio was observed in postlarval tiger shrimp provided with PHB substratum as compared to postlarvae provided with PVC substratum. Results indicate higher preference by postlarvae on PHB substratum over PVC substratum. Overall, this study indicates the potential of artificial substratum consisting of PHB‐based biodegradable plastic as replacement for conventional substratum consisting of PVC pipes in enhancing the survival of postlarval tiger shrimp and improving its performance against adverse environmental conditions and disease resistance.
CitationLudevese-Pascual, G., Laranja, J. L., Amar, E., Bossier, P., & De Schryver, P. (2019). Artificial substratum consisting of poly-β-hydroxybutyrate-based biodegradable plastic improved the survival and overall performance of postlarval tiger shrimp Penaeus monodon.
Pipes; Biodegradation; Disease resistance; Ammonium compounds; Shrimp culture; Survival; Environmental conditions; Substrata; Ammonium chloride; Plastics; Pathogens; Shellfish; Aquaculture; Biodegradable substances; Weight; Growth; Decapoda; Penaeus monodon; Artificial substratum; poly-β-hydroxybutyrate (PHB); Chloride resistance; Polyvinyl chloride; Ammonium; Polyhydroxybutyrate; Biodegradability; Vibrio; Waterborne diseases
This study was funded by the Flemish Interuniversity Council (VLIR).
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Oral presentationRP Ferraris, FDP Estepa, JM Ladja & EG de Jesus - In Y Taki, JH Primavera & JA Llobrera (Eds.), Proceedings of the First International Conference on the Culture of Penaeid Prawns/Shrimps, 4-7 December 1984, Iloilo City, Philippines, 1985 - Aquaculture Department, Southeast Asian Fisheries Development CenterThe osmotic, total protein and chloride ion regulation in two size groups (10 and 30 g) of Penaeus monodon Fabricius was investigated. Preliminary experiments showed that osmolality, total protein and chloride concentrations tend to become stable 24 to 36 hours after molting.Thus,hemolymph values 36 to 240 hours after sampling were not significantly different from each other. Based on these results, only 36 hours (or more) postmolt animals were sampled after transfer from control (32 ppt) to five test salinities (8, 16, 24, 32 and 40 ppt). Hemolymph samples were then taken 1, 2, 3, 5, 7 and 10 days after transfer. Results showed that in general, osmolality, total protein and chloride concentrations in the hemolymph did not vary with time within the same salinity.Both sizes exhibited hyperosmotic and hyperionic regulation in lower salinities and hypoosmotic and hypoionic regulation in higher salinities. The isosmotic values obtained were approximately 676 to 720 mOsm (24 to 28.8 ppt) for the 10 g, and 724 to 792 mOsm (26 to 28.5 ppt) for the 30 g size group. For chloride, the isoionic values ranged from 324 to 339 mM in 10 g prawns. Slopes of the regression lines of hemolymph osmolality versus salinity in 10 g prawns were not significantly different from slopes of similar regression lines in 30 g prawns. These results suggest that the ability to regulate osmotic and total protein concentration in the hemolymph is similar in the two size groups.
Adsorption and biomass concentration of thraustochytrid Schizochytrium aggregatum (Goldstein and Belsky) in Bunker C Oil BGS Sarinas, LD Gellada, MLT Torrigue, DN Sibonga, ES Torrato, JG Malagad, JG Feril, LAJ Bondoc, JCA Roncal & JA Tornalejo -
Journal of Environmental Science and Management, 2014 - School of Environmental Science and Management, University of the Philippines Los BañosDiverse array of microorganisms such as bacteria, fungi and protists are involved during oil spill. Each microorganism has its own specific function whether it has to degrade or adsorb hydrocarbons. One important microorganism is the Thraustochytrid that is a fungoid protist and are common in marine and estuarine habitats. Numerous studies existed on the biodegradation and adsorption of Thraustochytrids on various substances but not on Bunker C oil. Thus, this study aimed to determine the adsorption capacity and mean biomass of Thraustochytrids in Bunker C oil using different cell densities measured in grams. All of the three treatments or cell densities (1 x 105 cells ml-1, 1 x 106 cells ml-1 and 1 x 107 cells ml-1) were triplicated and average values were recorded. Oil dispersant was used as a control. It showed that Thraustochytrid with 1 x 107 cells ml-1 showed the highest adsorbed oil (.057 ḡ) among the three cell densities and showed significant difference at p = .01 but comparable to the control (.066 ḡ). In terms of biomass concentration, all cell densities showed no significant difference at p = .01. Thraustochytrid is a promising tool during oil spill because it has the capacity to adsorb oil.
Characteristics of dehalogenase from bacteria isolated from the gut of pond-reared rohu (Labeo rohita) juveniles in Myanmar E Abel, RV Pakingking Jr., G Pagador, MT Wint & F Huyop -
Advances in Bioscience and Biotechnology, 2012 - Scientific Research PublishingUnwarranted accumulation of halogenated compounds in the rivers and streams has in recent years emerged due to the widespread use agricultural pesticides. The presence of these halogenated compounds in the water does not only suppress the immune system of fish but adversely induce serious morbidity and mortality among cultured stocks. Importantly, gradual accumulation of these compounds in the system of cultured and wild freshwater fish species cultured in ponds and floating net-cages in dams and rivers, respectively, poses some risks to humans, the end users. In this study, we attempted to isolate bacteria from the gut of pond-reared rohu (Labeo rohita) in Myanmar, screened the isolated bacteria for dehalogenase gene using molecular technique and tested the ability of these bacteria to degrade halogenated compounds in vitro. The eight bacterial strains studied were identified as Enterobacter mori strain MK-121001, Enterobacter cloacae strains MK121003, MK-121004, MK121010, Ralstonia solanacearum strain 121002, Acinetobacter baumannii strain MK121007, Chromobacterium violaceum strain MK121009 and Pantoea vagans strain 121011. Only three bacterial strains (MK121002, MK121007 and MK121009) were capable of degrading 2,2-dichloropropionic acid (2,2-DCP) as the sole carbon source up to a final substrate concentration of 20 mM. Their mean growth doubling time ranging from 6 - 23 hours with the maximum of chloride ion released of 85%. PCR amplification with oligonucleotide primers designed from group I dehalogenase revealed the presence of dehalogenase genes in all isolates suggesting dehalogenase gene in strains 121001, 121003, 121004, 121010 and 121011 were silenced. In contrast, group II dehalogenase primers did not show any PCR amplification. These results suggest that MK121002, MK121007 and MK121009 only encode a group I dehalogenase and its non-stereoselectivity is in agreement with previoulsly described group I haloacid dehalogenase. The partial gene sequences were blasted but no significant sequence identity was observed. Therefore, it suggests the 2-haloacid dehalogenase of MK121002, MK12-1007 and MK- 121009 might be a novel group I 2-haloacid dehalogenase. The results indicated a broad distribution of dehalogenation genes in many microbial genomes that harbor dehalogenase(s) due to the exposure of the microorganisms to the naturally occurring or man-made halogenated compounds in the environmental systems. So far, microorganisms capable of producing dehalogenases were mainly isolated from soil and scarcely from aquatic animals and their environments. To the authors’ knowledge, this is the first report on the isolation of dehalogenase-producing bacteria from the gut of pond-reared freshwater fish, Labeo rohita, in Myanmar.