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Genomic analysis of Vibrio harveyi strain PH1009, a potential multi-drug resistant pathogen due to acquisition of toxin genes
(Elsevier, 2023-03-25)
In has increasingly been observed that viral and bacterial coinfection frequently occurs among cultured shrimp and this coinfection could exacerbate the disease phenotype. Here, we describe a newly discovered bacterial strain, Vibrio harveyi PH1009 collected from Masbate Island, Philippines that was found to be co-infecting with the White Spot Syndrome virus a sample of black tiger prawn, Penaeus monodon. The genome of V. harveyi PH1009 was sequenced, assembled, and annotated. Average Nucleotide identity calculation with Vibrio harveyi strains confirmed its taxonomic identity. It is a potential multi-drug and multi-heavy metal resistant strain based on the multiple antibiotic and heavy metal resistance determinants annotated on its genome. Two prophage regions were identified in its genome. One contained genes for Zona occludens toxin (Zot) and Accessory cholera toxin (Ace), essential toxins of toxigenic V. cholerae strains apart from CTX toxins. Pan-genome analysis of V. harveyi strains, including PH1009, revealed an “open” pan-genome for V. harveyi and a core genome mainly composed of genes necessary for growth and metabolism. Phylogenetic tree based on the core genome alignment revealed that PH1009 was closest to strains QT520, CAIM 1754, and 823tez1. Published virulence factors present on the strain QT520 suggest similar pathogenicity with PH1009. However, PH1009 Zot was not found on related strains but was present in strains HENC-01 and CAIM 148. Most unique genes found in the PH1009 strain were identified as hypothetical proteins. Further annotation showed that several of these hypothetical proteins were phage transposases, integrases, and transcription regulators, implying the role of bacteriophages in the distinct genomic features of the PH1009 genome. The PH1009 genome will serve as a valuable genomic resource for comparative genomic studies and in understanding the disease mechanism of the Vibrio harveyi species....
Microsatellite and mitochondrial haplotype diversity reveals population differentiation in the tiger shrimp (Penaeus monodon) in the Indo-Pacific region
(International Society for Animal Genetics, 2008)
The black tiger shrimp (Penaeus monodon) is an ecologically and economically important penaeid species and is widely distributed in the Indo-Pacific region. Here we investigated the genetic diversity of P. monodon (n = ...
The response of Penaeus monodon juveniles to varying protein/energy ratios in test diets
(Elsevier, 1986)
Two sets of factorial experiments were conducted for 8 weeks to determine the response of Penaeus monodon juveniles (average weights = 0.60 ± 0.16 g and 0.80 ± 0.05 g) to diets containing various protein/energy ratios. The ...
Effect of different sizes of saline red tilapia hybrid Oreochromis niloticus Linnaeus x O. mossambicus Peters on the growth of luminous bacteria Vibrio harveyi
(College of Agriculture, University of the Philippines Los Baños, 2010)
Luminous bacterial disease caused by Vibrio harveyi has devasted the shrimp industry. The use of different strains of tilapia and other fish species polycultured with shrimp cultured at a salinity of 24 ppt to control ...
Growth and mid-gut cells profile of Penaeus monodon juveniles fed water-soluble-vitamin deficient diets
(Elsevier, 1989)
Growth and changes in the mid-gut cell morphology of Penaeus monodon juveniles were evaluated after feeding for 35 days with semi-purified diets deficient in water-soluble vitamins. Diets were prepared by deleting one ...
Successful spawning of cultured Penaeus monodon Fabricius after eyestalk ablation
(Elsevier, 1977)
Preliminary experiments resulted in successful spawning of captive stock of the prawn Penaeus monodon, or “sugpo”, to complete the life cycle and produce a viable F1 generation of larvae. Gonadal development was induced ...
Polyculture of green mussels, brown mussels and oysters with shrimp control luminous bacterial disease in a simulated culture system
(Elsevier, 2007)
Shrimp mortality due to luminous bacteria has been a problem of the shrimp industry worldwide. Polyculture of shrimp with finfish, such as grouper, seabass, snapper, siganid, Tilapia hornorum, and the Genetically Improved ...
Apparent digestibility of diets with various carbohydrate levels and the growth response of Penaeus monodon
(Elsevier, 1991)
The digestibility of four isonitrogenous practical diets (40% crude protein) containing different levels (5%, 15%, 25% and 35%) of gelatinized breadflour as carbohydrate source were determined for P. monodon (average weight ...
Investigation of some components of the greenwater system which makes it effective in the initial control of luminous bacteria
(Elsevier, 2003)
Two components of the greenwater system, the tilapia water and Chlorella, were investigated separately for their effect on the population of luminous bacteria. For the investigation of tilapia water, Tilapia hornorum was ...
Black tiger shrimp culture rejuvenation: the OPLAN Balik Sugpo of SEAFDEC/AQD
(Secretariat, Southeast Asian Fisheries Development Center, 2021)
Back in its glory days, black tiger shrimp was an economic jewel of the Philippines and other countries in Asia that garnered millions of dollars’ worth of earnings from production and export. Until production methods ...