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Establishment of preventive measures against viral nervous necrosis (VNN) in finfish hatcheries

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delaPenaLD2005.pdf (342.2Kb) AQD Access AQD Access
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Date
2005-03
Author
de la Peña, Leobert D.
Cruz-Lacierda, Erlinda R.
Baguisi, Kathrine Marie S.
Napa, Jane Frances
Sombito, Christopher D.
Tendencia, Eleonor ORCID
Paner, Milagros G.
Page views
4,910
Subject
hatcheries ASFA
necrosis ASFA
mariculture ASFA
fish culture ASFA
fish diseases ASFA
histopathology ASFA
breeding stock ASFA
viruses ASFA
nucleic acids ASFA
fish eggs ASFA
disinfection ASFA
trash fish ASFA
cell culture ASFA
disease prevention AGROVOC
Epinephelus coioides AGROVOC
Lates calcarifer AGROVOC
Lutjanus argentimaculatus AGROVOC
Chanos chanos AGROVOC
Taxonomic term
Chanos chanos GBIF
Epinephelus fuscoguttatus GBIF
Lates calcarifer GBIF
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Abstract
The establishment of preventive measures against viral nervous necrosis (VNN) at the finfish hatcheries of SEAFDEC Aquaculture Department in Iloilo, Philippines, was instituted primarily to minimize the impact of the viral infection in cultured stocks. Selection of VNN-negative broodstocks was conducted and the detection rate ranged from 25-56%. It was confirmed that all species of broodstocks are already contaminated with the virus with varying percentages. The combination of cell culture and RT-PCR in the detection of the virus proved to be more sensitive than the RT-PCR alone. The trash fish food was suspected as a main source of contamination and tests prove that most of the trash fish species were contaminated with the virus. The effect of chemical treatment was tested and a good result was obtained by using 5 and 7 ppm iodine. Several recommendations were formulated in the prevention and control of VNN in the hatchery phase: VNN-negative broodstocks should be selected by using cell culture and PCR; spawning of the selected broodstocks should be limited to only six times and these fertilized eggs should be used for larval productions; fertilized eggs should be washed and disinfected with iodine; and commercial broodstock feeds should be used instead of trash fish.
URI
http://hdl.handle.net/10862/5919
Suggested Citation
de la Peña, L. D., Cruz-Lacierda, E. R., Baguisi, K. M. S., Napa, J. F., Sombito, C. D., Tendencia, E. A., & Paner, M. G. (2005). Establishment of preventive measures against viral nervous necrosis (VNN) in finfish hatcheries. In K. Nagasawa (Ed.), Recent Advances in Diagnosis and Prevention of Fish and Shrimp Diseases in Southeast Asia (pp. 115–125). Tigbauan, Iloilo, Philippines: Aquaculture Department, Southeast Asian Fisheries Development Center.
Type
Book chapter
ISBN
9718511732
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  • Recent Advances in Diagnosis and Prevention of Fish and Shrimp Diseases in Southeast Asia [43]

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