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Changes in mRNA expression of grouper (Epinephelus coioides) growth hormone and insulin-like growth factor I in response to nutritional status

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Date
2006
Author
Pedroso, Fiona L.
De Jesus-Ayson, Evelyn Grace T.
Cortado, Hanna H.
Hyodo, Susumu
Ayson, Felix G.
Page views
2,981
ASFA keyword
hormones ASFA
starvation ASFA
nutrition ASFA
endocrinology ASFA
growth rate ASFA
food availability ASFA
marine fishes ASFA
condition factor ASFA
gene expression ASFA
liver ASFA
amino acids ASFA
polymerase chain reaction ASFA
primers ASFA
AGROVOC keyword
somatotropin AGROVOC
insulin-like growth factor AGROVOC
nutritional status AGROVOC
open reading frames AGROVOC
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Abstract
Growth hormone (GH) and insulin-like growth factor-I (IGF-I) are key links to nutritional condition and growth regulation in teleost. To understand the endocrine mechanism of growth regulation in grouper, we cloned the cDNAs for grouper GH and IGF-I and examined their mRNA expression during different nutritional status. Grouper GH cDNA is 936 base pairs (bp) long excluding the poly-A tail. It contained untranslated regions of 85 and 231bp in the 5'- and 3'-ends, respectively. It has an open reading frame of 612bp coding for a signal peptide of 17 amino acids (aa) and a mature hormone of 187aa residues. Based on the aa sequence of the mature hormone, grouper GH shows higher sequence identity (>76%) to GHs of perciforms than to GHs of cyprinids and salmonids (53-69%). Grouper preproIGF-I cDNA consisted of 558bp, which codes for 186aa. This is composed of 44aa for the signal peptide, 68aa for the mature peptide comprising B, C, A, and D domains, and 74aa for the E domain. Mature grouper IGF-I shows very high sequence identity to IGF-I of teleost fishes (84-97%) compared to advanced groups of vertebrates such as chicken, pig, and human (=<80%). Using DNA primers specific for grouper GH and IGF-I, the changes in mRNA levels of pituitary GH and hepatic IGF-I in response to starvation and refeeding were examined by a semi-quantitative RT-PCR. Significant elevation of GH mRNA level was observed after 2 weeks of food deprivation, and increased further after 3 and 4 weeks of starvation. GH mRNA level in fed-controls did not change significantly during the same period. Hepatic IGF-I mRNA level decreased significantly starting after 1 week of starvation until the 4th week. There was no significant change in IGF-I mRNA levels in fed-controls. One week of refeeding can restore the GH and IGF-I mRNA back to its normal levels. Deprivation of food for 1-4 weeks also resulted in cessation of growth and decrease in condition factor.
URI
http://hdl.handle.net/10862/94
Suggested Citation
Pedroso, F. L., De Jesus-Ayson, E. G. T., Cortado, H. H., Hyodo, S., & Ayson, F. G. (2006). Changes in mRNA expression of grouper (Epinephelus coioides) growth hormone and insulin-like growth factor I in response to nutritional status. General and Comparative Endocrinology, 145(3), 237-246. https://doi.org/10.1016/j.ygcen.2005.09.001 
DOI
10.1016/j.ygcen.2005.09.001
Type
Article
ISSN
0016-6480
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  • Journal Articles [1249]

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