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dc.contributor.authorBautista-Teruel, Myrna N.
dc.contributor.authorKoshio, Shunsuke S.
dc.contributor.authorIshikawa, Manabu
dc.date.accessioned2012-06-13T11:40:12Z
dc.date.available2012-06-13T11:40:12Z
dc.date.issued2011
dc.identifier.citationBautista-Teruel, M.N., Koshio, S.S., Ishikawa, M. (2011). Diet development and evaluation for juvenile abalone, Haliotis asinina Linne: Lipid and essential fatty acid levels. Aquaculture 312(1-4):172-179en
dc.identifier.issn0044-8486
dc.identifier.urihttp://hdl.handle.net/10862/983
dc.description.abstractExperiments on diet development and evaluation for juvenile abalone, Haliotis asinina focusing on lipid and essential fatty acid (EFA) levels were conducted. Six isonitrogenous diets were formulated in Experiment 1 (E1) to contain 27% protein with lipid levels at 0, 2, 4, 6, 8, and 10%. Experiment 2 (E2) (EFA levels), used the optimum lipid level (3.59%) in E1 with EFA supplementation of 0.0, 0.2, 0.4, 0.8, and 1.6%. Abalone juveniles [mean initial weight and shell length of 0.60 plus or minus 0.07g and 14.70 plus or minus 0.12mm (E1)], [0.60 plus or minus 0.16g and 15.30 plus or minus 0.73mm (E2)] respectively, were fed these diets at 2-5% body weight in 3 replicates. Feeding trials in 90days/experiment evaluated growth, survival, feed conversion ratio (FCR) and fatty acid composition in abalone tissues. Results showed significantly higher growth rates (ANOVA P<0.05) with abalone fed diets with lipid levels of 2.2%, 3.6%, and 6.1% compared with those containing lipid levels of 7.6% and 9.8%. Abalone fed the lipid-free diet showed significantly the lowest growth rate among treatments. Break point analysis as a function of growth, showed optimum lipid requirement at 3.59%. Survival was high at 95-99% in both experiments. FCR values for D3 and D4 were significantly better compared to D2, D5 and D6 (E1) while no significant differences were found for D2-D6 for E2. Abalone body lipid increased with corresponding increase in dietary lipid. Addition of 18:2n, 18:3n3, and n3 HUFA showed significant improvement in weight gains up to 1.6% supplementation. Fatty acid composition of the lipid samples reflected those of the diets. Total lipid of abalone fed the lipid-free diet showed higher monoenes. Addition of EFA resulted in an increase in both n3 and n6 fatty acids. Lipid incorporation at 3.6% using a 1:1 ratio of CLO and SBO with EFA supplementation (1.6%) is best in juvenile abalone diet formulation.en
dc.language.isoenen
dc.publisherElsevieren
dc.subjectAbalonesen
dc.subjectHaliotis asininaen
dc.subjectLipidsen
dc.subjectEssential fatty acidsen
dc.titleDiet development and evaluation for juvenile abalone, Haliotis asinina Linne: Lipid and essential fatty acid levelsen
dc.typeArticleen
dc.date.updated2012-06-13T03:53:58Z
dc.identifier.doi10.1016/j.aquaculture.2011.01.004
dc.citation.volume312
dc.citation.issue1-4
dc.citation.spage172
dc.citation.epage179
dc.citation.journalTitleAquacultureen


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