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dc.contributor.authorCaipang, Christopher M. A.
dc.contributor.authorPakingking Jr., Rolando V.
dc.contributor.authorApines-Amar, Mary Jane S.
dc.contributor.authorHuyop, Fahrul
dc.contributor.authorBautista, Norwell B.
dc.date.accessioned2012-06-19T05:59:26Z
dc.date.available2012-06-19T05:59:26Z
dc.date.issued2011
dc.identifier.citationCaipang, C. M. A., Pakingking Jr., R. V., Apines-Amar, M. J. S., Huyop, F., & Bautista, N. B. (2011). Development of a polymerase chain reaction (PCR) assay targeted to the dnaJ gene of Vibrio harveyi, a bacterial pathogen in Asian seabass, Lates calcarifer. Aquaculture, Aquarium, Conservation and Legislation, 4(4):447–454.en
dc.identifier.issn1844-8143
dc.identifier.urihttp://hdl.handle.net/10862/988
dc.description.abstractPartial sequence of the dnaJ gene of Vibrio harveyi, which was isolated from diseased juvenile Asian seabass, Lates calcarifer was identified. The partial sequence of dnaJ gene of V. harveyi was 447 bp and shared at least 77% identity at the nucleotide level with the dnaJ gene of other Vibrios. It was distinct from the dnaJ gene of other Vibrios but was closely related with the dnaJ gene of V. rotiferianus and V. campbellii having at least 90% nucleotide identity. PCR primers targeting this gene were designed to detect the pathogen in Asian seabass. The assay was specific to V. harveyi and the limit of detection was 100 pg of genomic DNA ml-1 or 100 fg of bacterial genomic DNA in a PCR reaction. This corresponded to a sensitivity of approximately 20 genome equivalents (GE) of V. harveyi. These results indicate that the dnaJ gene is a good candidate to develop primers for the PCR assay in detecting V.harveyi in fish.en
dc.language.isoenen
dc.publisherBiofluxen
dc.relation.urihttp://www.bioflux.com.ro/docs/2011.4.447-454.pdf
dc.subjectVibrio harveyien
dc.subjectdnaJ geneen
dc.subjectLates calcariferen
dc.subjectPCRen
dc.titleDevelopment of a polymerase chain reaction (PCR) assay targeted to the dnaJ gene of Vibrio harveyi, a bacterial pathogen in Asian seabass, Lates calcariferen
dc.typeArticleen
dc.citation.volume4
dc.citation.issue4
dc.citation.spage447
dc.citation.epage454
dc.citation.journalTitleAquaculture, Aquarium, Conservation and Legislationen


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