SEAFDEC/AQDINSTITUTIONAL REPOSITORY
    • Login
    View Item 
    •   SEAFDEC/AQD Institutional Repository Home
    • 03 SEAFDEC/AQD External Publications
    • Journal Articles, Conference Papers and Book Chapters by SEAFDEC Staff
    • Journal Articles
    • View Item
    •   SEAFDEC/AQD Institutional Repository Home
    • 03 SEAFDEC/AQD External Publications
    • Journal Articles, Conference Papers and Book Chapters by SEAFDEC Staff
    • Journal Articles
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Leucaena leucocephala leaves in formulated feed for Penaeus monodon: a concrete example of the application of histology in nutrition research

    • Global styles
    • APA
    • Elsevier - Harvard
    • Vancouver
    • MLA
    • Help
    Thumbnail
    View/Open
    Request a copy
    Date
    1986
    Author
    Vogt, Günter
    Quinitio, Emilia T.
    Pascual, Felicitas P.
    Page views
    1,490
    Metadata
    Show full item record

    Cited times in Scopus

    
    
    Share 
     
    Abstract
    Penaeus monodon postlarvae were fed with formulated diets containing either soaked or unsoaked Leucaena leaves. A similarly prepared feed with soybean in place of Leucaena was used for comparison. Results were analysed by statistical and histological methods.

    Results of the 4-week feeding experiment showed that mean weight gains and percentage survival of the prawns fed with the two Leucaena-containing diets were higher, but not significantly different from soybean-containing diet. The content of the poisonous amino acid mimosine in the Leucaena leaves could be reduced about 70% by soaking the leaves in freshwater for 24 h. Highest weight gain was obtained with the feed containing these soaked leaves.

    In addition to growth and survival, the R-cells of the midgut glands of the postlarvae were investigated by light and electron microscopy. Although the statistical growth values and the proximate analyses of the test diets were similar, a food-specific ultrastructure was established after only 4 days of feeding. All diets resulted in subcellular characteristics typical for well fed prawns and, at that time, the Leucaena diets were already slightly superior to the soybean control. This indicates that different sources of macronutrients lead to different ultrastructures even if the proximate analyses of protein, carbohydrate and lipid are similar.

    Only slight changes in the R-cells were observed after 11, 20 and 28 days in the prawns fed with the soybean diet compared to 4 days of feeding. In the diet containing unsoaked Leucaena leaves, however, many R-cells became heavily damaged after 20 and 28 days, whereas the prawns fed with the diet containing soaked leaves exhibited less pronounced distortion. Statistical analyses of growth and survival rate did not show these adverse effects at that time. Although it is highly probable that the mimosine is responsible for those pathological symptoms, complementary experiments could not clearly prove that.

    The effects of feed components are visible on the cellular or organ level after only a few days, whereas the individuals (organism level) reflect them about 10 days later. Another 10 days later the changes are manifested in the population. Therefore it is suggested to use histology in nutrition studies as a supplementary source of information to statistical and biochemical parameters. The midgut glands can further be used to monitor the nutritional condition of prawns in aquaculture, sea ranching, and in ecological investigations.

    The study confirms that Leucaena leaves are a promising protein source for prawn diets if mimosine could be reduced to a very low level. A mimosine level of 0.25% in the feed is still too high, if the diet is used uninterruptedly for several weeks.
    Description
    Contribution No. 187 of the Aquaculture Department of SEAFDEC.
    URI
    http://hdl.handle.net/10862/1203
    Suggested Citation
    Vogt, G., Quinitio, E. T., & Pascual, F. P. (1986). Leucaena leucocephala leaves in formulated feed for Penaeus monodon: a concrete example of the application of histology in nutrition research. Aquaculture, 59(3-4), 209-234.
    DOI
    10.1016/0044-8486(86)90005-0
    Type
    Article
    ISSN
    0044-8486
    Subject
    Nutritional requirements; Artificial feeding; Digestive system; Shrimp culture; Histology; Growth; Leucaena leucocephala; Penaeus monodon
    Collections
    • Journal Articles [1108]


    © SEAFDEC/AQD  2021
    Contact Us | Send Feedback
     

     

    Browse

    All of SAIRCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsKeywordsThis CollectionBy Issue DateAuthorsTitlesSubjectsKeywords

    My Account

    LoginRegister

    Statistics

    View Usage Statistics
    SEAFDEC/AQD Library

    © SEAFDEC/AQD  2021
    Contact Us | Send Feedback
     

     

    Export citations

    Export the current results of the search query as a citation list. Select one of the available citation styles, or add a new one using the "Citations format" option present in the "My account" section.

    The list of citations that can be exported is limited to items.

    Export citations

    Export the current item as a citation. Select one of the available citation styles, or add a new one using the "Citations format" option present in the "My account" section.

    Export Citations

    EXTERNAL LINKS DISCLAIMER

    This link is being provided as a convenience and for informational purposes only. SEAFDEC/AQD bears no responsibility for the accuracy, legality or content of the external site or for that of subsequent links. Contact the external site for answers to questions regarding its content.

    If you come across any external links that don't work, we would be grateful if you could report them to the repository administrators.

    Click DOWNLOAD to open/view the file. Request a copy in case the link we provided don't work.

    Download

    DOCUMENT REQUEST NOT AVAILABLE

    This publication is still available (in PRINT) and for sale at AQD bookstore. The library is currently restricted to send PDF of publications that are still for sale.

    You may contact bookstore@seafdec.org.ph or visit AQD bookstore for orders.

    FILE UNDER EMBARGO

    This file associated with this publication is currently under embargo. This will be available for download after the embargo date.