An inexpensive tag for short-term studies in milkfish (Chanos chanos Forsskal) and in seabass (Lates calcarifer Bloch)
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An opercular tag for marking adult milkfish (Chanos chanos Forsskal) and seabass (Lates calcarifer Bloch) is described. High tag retention and relatively low mortality rates were observed in adult fish handled two to ten times during 14-to 60-day tests. The features and advantages of the tag for marking large-sized fish in short-term studies are discussed.
CitationGarcia, L. M. B., & Gapasin, R. S. J. (1988). An inexpensive tag for short-term studies in milkfish (Chanos chanos Forsskal) and in seabass (Lates calcarifer Bloch).
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Conference paperLV Benitez - In RD Fortes, LC Darvin & DL de Guzman (Eds.), Fish and crustacean feeds and nutrition : Proceedings of the seminar-workshop on fish and crustacean feeds and nutrition held on 25-26 February 1985 at UPV, Iloilo City, 1989 - Philippine Council for Aquatic and Marine Research and DevelopmentThis paper reviews recent work on milkfish nutrition. Substantial progress had been made towards understanding the digestive physiology of milkfish. Major enzaymes envolved in the digestions of carbohydrates, protein and lipids had been detected in the pyloric caece, intestines and pancreas of milkfish. The most active carbohydrates were involved in the hydrolysis of α - glocosidic bonds. Intestinal amylase activity consistently reached the peak at about noon when milkfish gut was full. This confirms that milkfish is s daytime feeder. No cellulase activity was detected in any region orf the digertive treat although the fish relies heavily algae and other plant source for food. Trypsin, chymotrypsin and general proteases were also detected in milkfish digestive tract. A powerful milkfish trypsin inhabitor was detected in the filementous algae, Chaetomorpha brachygona which is predominant species in lumot. Lipass in the pancreas and intestines had two pH optima, suggesting a physiologic versatility for lipid digestion in milkfish. There is a limit information on the nutrient requirement of milkfish. Most studies showed that milkfish fry has a dietary requirement of 40% protein, and 7-10 lipid. Studies on the protein-energy requirement of fingerlings suggested that 30-40% protein, 10% fat and 25% carbohydrates are required. Subsequent studies showed an optimum protein energy to total metabolizable energy ratio of 44.4%. Amino acid test diets for milkfish had been formulated to contain white fish meal, gelatin and approprate amino acid mix.
Lactate dehydrogenase isozyme patterns during the development of milkfish, (Chanos chanos (Forskal)) PD Requintina, LM Engle & LV Benitez -
Kalikasan, The Philippine Journal of Biology, 1981 - University of the Philippines at Los BañosPolyacrylamide disc gel electrophoresis was done to determine the lactate dehydrogenase (LDH) isozyme patterns for fry (5-3 mg), fingerling (6-12 g), pond-size (150-250 g) and adult (6-9 kg) milkfish. The patterns were tissue specific; the different tissues examined, viz., eye, liver, heart, and skeletal muscle had different expressions of LDH isozymes. The resolved patterns appeared to be products of LDH gene loci A, B, and C. Subunits A and B were present in all tissues. A4 and B4 were predominant in skeletal and heart muscle, respectively; the two associated non-randomly in vivo and formed only the heteropolymers A3B and AB3. A liver band, L4, was most conspicuous in the fingerling, pond-size, and adult; it was assumed to be coded by locus C. A negatively charged band, X4, was detected in fully developed ovary and in fry homogenized as whole individuals, but it could not be resolved in tissues of fingerling. Six-mo old stunts and 3-mo old fingerlings had similar LDH patterns for all tissues examined. The patterns for 11-mo old stunts and fingerlings also were similar but the one for the eye of the former was the same pattern resolved for the eye of adults. There was no change in the LDH isozyme patterns of milk fish stunted for 6 mo under different salinity levels (0-5, 15-20, 32-35 ppt).
Conference paperGF Quinitio & MN Duray - In CL Marte, GF Quinitio & AC Emata (Eds.), Proceedings of the Seminar-Workshop on Breeding and Seed Production of Cultured Finfishes in the Philippines, Tigbauan, Iloilo, Philippines, 4-5 May 1993, 1996 - Aquaculture Department, Southeast Asian Fisheries Development CenterResearch on seed production of several foodfishes has been a continuing activity of SEAFDEC/AQD since 1976. Fry and juvenile production methods of these fish commodities are in various stages of advancement. For instance, advances in the development of hatchery rearing, particularly feeding and water management schemes, have made mass production of milkfish (Chanos chanos) seed a reality, resulting further in the application of the technology in commercial hatcheries. Recent studies now focus on assessing the quality of hatchery seed stocks of milkfish vis-a-vis wild seed during nursery and grow-out culture. Likewise, sea bass (Lates calcarifer) seed production has undergone significant improvements since the technology was introduced in the Philippines in 1982. Fatty acid-enrichment of a zooplankton diet can enhance growth and survival of sea bass fry, although other cheaper alternatives and early weaning to formulated diet preparations are currently being tested. Hatchery fry production of grouper (Epinephelus salmoides and E. suillus syn. E. coioides) and snapper is in its infancy, but trials complemented by research on their larval feeding habits and requirements are underway to establish reliable methods of rearing larvae of these species. Although fairly well-established, seed production of rabbitfish (Siganus guttatus) requires further improvement in determining an appropriate zooplankton diet to ensure adequate growth and survival of larvae. Hatchery fry production of tilapia (Oreochromis sp.), carps (Aristichthys nobilis, Hypothalmichthys molitrix) and, to a certain extent, catfish (Clarias macrocephalus) can already be categorized as a flourishing industry in some parts of the Philippines. Nonetheless, SEAFDEC/AQD continues to conduct research on these freshwater species, with particular emphasis on nutrition and feed development during the nursery production phase. Together, results of past and on-going research studies ensure that seed supply of these important foodfishes become adequate and sustainable for the grow-out.