Laboratory Manual of Standardized Methods for Antimicrobial Sensitivity Tests for Bacteria Isolated from Aquatic Animals and Environment

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This manual is one of the important outputs of a collection of studies related to antibiotic usage in order to come up with guidelines for its prudent usage. It offers a complete guide for testing bacterial susceptibility and resistance through the use of simple techniques for disk agar diffusion tests, and a guide to do a more thorough study to test therapeutic levels using microbial inhibitory concentration.

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  • Minimal inhibitory concentration (MIC) test and determination of antimicrobial resistant bacteria 

    Ruangpan, Lila (Aquaculture Department, Southeast Asian Fisheries Development Center, 2004)
    The agar dilution technique is used to measure qualitatively the in vitro activity of an antimicrobial agent against the test bacteria. In this method, graded amounts of antibiotics are incorporated in agar plates and ...

  • Disk diffusion method 

    Tendencia, Eleonor ORCID (Aquaculture Department, Southeast Asian Fisheries Development Center, 2004)
    Disk diffusion method is based on the principle that antibiotic-impregnated disk, placed on agar previously inoculated with the test bacterium, pick-up moisture and the antibiotic diffuse radially outward through the agar ...

  • Bacterial isolation, identification and storage 

    Ruangpan, Lila; Tendencia, Eleonor ORCID (Aquaculture Department, Southeast Asian Fisheries Development Center, 2004)
    Bacterial isolation, purification and identification are the first steps to bacteriological studies. Isolation is done to obtain pure bacterial cultures. Bacteria are usually isolated from fish kidney and spleen; and from ...

  • Laboratory manual of standardized methods for antimicrobial sensitivity tests for bacteria isolated from aquatic animals and environment 

    Ruangpan, Lila; Tendencia, Eleonor ORCID (Aquaculture Department, Southeast Asian Fisheries Development Center., 2004)
    The manual is one of the important outputs of a collection of studies related to antibiotic usage in order to come up with guidelines for its prudent usage. It offers a complete guide for testing bacterial susceptibility ...