Optimization of culture conditions for tissue culture production of young plantlets of carrageenophyte Kappaphycus
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To improve the production of Kappaphycus plantlets in tissue culture, optimum media concentrations of an Ascophyllum nodosum extract (Acadian Marine Plant Extract Powder, AMPEP), plant growth regulators (PGR), pH–temperature combinations, and explant density were determined. Kappaphycus alvarezii var. tambalang purple (PUR), kapilaran brown (KAP), vanguard brown (VAN), adik-adik (AA), tungawan green (TGR), and K. striatum var. sacol green (GS) were used as explants. Based on the shortest period for shoot emergence and the economical use of AMPEP, the optimum enriched media was 3.0 mg L−1 AMPEP and 0.1 mg L−1 AMPEP + PGR 1 mg L−1 each phenylacetic acid (PAA) and zeatin for PUR, 1.0 mg L−1 AMPEP + PGR for KAP and GS, 0.1 mg L−1 AMPEP + PGR for VAN, and 3.0 mg L−1 AMPEP and 0.001 mg L−1 AMPEP + PGR for AA and TGR. Results showed that the addition of PGR to low concentrations of AMPEP hastened shoot formation. pH–temperature combinations for the most rapid shoot formation were determined for the brown (KAP) and purple (PUR) color morphotypes of K. alvarezii var. tambalang and the green morphotype of K. striatum var. sacol (GS) cultured in 1.0 mg L−1 AMPEP + PGR. The brown morphotype produced the most number of shoots at pH 7.7 at 20°C after as little as 20 days. Purple K. alvarezii showed an increased shoot formation at pH 6.7 at 25°C and the green K. striatum morphotype at pH 8.7 at 25°C. The optimum number of explants added to the culture media was also determined for tungawan green (TGR), brown (KAP), and tambalang purple (PUR) varieties of K. alvarezii in 1.0 mg L−1 AMPEP + PGR. The number of explants and the volume of the culture media combination were also tested. The highest average number of shoots formed occurred in two explants:1 mL culture media (2:1) for KAP and PUR (35.00% and 16.67%, respectively) and 1 explant: 2 mL culture media for the TGR (100.00%) with a range of 0.5–3.0 mm shoot length after 40 days in culture. The earliest shoot formation was observed after 21 days for the brown and 9 days for both the green and purple color morphotypes of Kappaphycus, in all densities investigated. This indicated that within the range tested, the density of explants did not have a significant effect on the rate of shoot formation but did influence the average number generated from the culture. The rate of production of new and improved Kappaphycus explants for a commercial nursery stock was improved through the use of AMPEP with optimized culture media pH, temperature, and density conditions.
CitationYunque, D. A. T., Tibubos, K. R., Hurtado, A. Q., & Critchley, A. T. (2011). Optimization of culture conditions for tissue culture production of young plantlets of carrageenophyte Kappaphycus.
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Conference paperIbA Kechik - In TU Bagarinao & EEC Flores (Eds.), Towards sustainable aquaculture in Southeast Asia and Japan: Proceedings of the Seminar-Workshop on Aquaculture Development in Southeast Asia, Iloilo City, Philippines, 26-28 July, 1994, 1995 - Aquaculture Department, Southeast Asian Fisheries Development CenterAquaculture in Malaysia is experiencing rapid growth. Total production in 1992 amounted to 79,699 tons valued at RM 207.4 million. These figures are 23% and 25% higher than the previous year's. Semi-culture of the cockle Anadara granosa was still predominant, contributing about 70% of the total output. Culture and production of the oyster Crassostrea iredalei is still insignificant. Sea bass Lates calcarifer constituted over 80% of the production from marine cages. Cage culture of grouper Epinephelus sp., snapper Lutjanus sp. and pompano Trachinotus blochii were also done in much smaller scale. The mangrove snapper Lutjanus argentimaculatus was recently spawned in captivity and larvae and juveniles were produced. In 1992, the tiger shrimp Penaeus monodon constituted about 87% of brackishwater pond production. Pond culture of the white shrimp P. merguiensis and the mudcrab Scylla sp. is at the experimental stage. Red tilapia hybrid was the major freshwater species cultured in cages, with 1,486 tons harvested in 1992. Freshwater pond production was valued at RM 100.85 million, 22% of which was due to the eel Anguilla japonica. Production of freshwater ornamental fishes is also becoming significant. Other exotic species recently bred and cultured are the African catfish Clarias gariepinus and the pacu Piaractus brachypomus. The indigenous freshwater catfish Mystus nemurus and carp Probarbus julleini have recently been bred in captivity and cultured experimentally. Lately, there have been attempts to culture non-conventional species such as the bullfrog Rana catesbeiana, the soft-shell turtle Trionyx sinensis, and aquatic ornamental plants.
Conference paperS Sahavacharin - In TU Bagarinao & EEC Flores (Eds.), Towards sustainable aquaculture in Southeast Asia and Japan: Proceedings of the Seminar-Workshop on Aquaculture Development in Southeast Asia, Iloilo City, Philippines, 26-28 July, 1994, 1995 - Aquaculture Department, Southeast Asian Fisheries Development CenterCoastal aquaculture in Thailand has expanded rapidly in both area and production in the last decade. The important cultured species are the shrimps (Penaeus monodon and P. merguiensis), sea bass Lates calcarifer, groupers Epinephelus malabaricus and E. tauvina, green mussel Perna viridis, horse mussel Modiolus senhausenii, blood cockles Anadara granosa and A. nodifera and the oysters Crassostrea belcheri, C. lugubris and Saccostrea commercialis. The total production from coastal aquaculture in 1991 was 230,444 tons, consisting of 70.3% shrimp, 28.8% mollusks, and 0.9% fishes. The seaweeds Gracilaria spp., pearl oysters, scallops, and abalones are cultured on a pilot scale in some places. Hatchery technologies have recently been developed for groupers, oysters, scallops, and abalones. Expanded aquaculture has had some adverse effects on the environment and has also suffered from the environmental changes and conflicts due to other sectors using the same water and other resources.
Conference paperSM Aypa - In TU Bagarinao & EEC Flores (Eds.), Towards sustainable aquaculture in Southeast Asia and Japan: Proceedings of the Seminar-Workshop on Aquaculture Development in Southeast Asia, Iloilo City, Philippines, 26-28 July, 1994, 1995 - Aquaculture Department, Southeast Asian Fisheries Development CenterAquaculture is regarded as the most promising source of protein food in the years ahead. Milkfish and Nile tilapia are the major fishes now produced but groupers, sea bass, rabbitfish, red snappers, carps, and catfishes are grown by some farmers. The tiger shrimp is still the most important cultured crustacean, but white shrimps and mudcrabs also have great potential. Oysters and mussels are produced in considerable amounts. Mariculture of the seaweed Eucheuma is now a well established industry, and the pond culture of Gracilaria for agar extraction is beginning to take off.