Diagnostic and preventive practices for iridovirus in marine fish
NakajimaK2002-Diagnostic-and-Preventive-Practices-for-Iridovirus-in-Marine-Fish.pdf (201.2Kb)Abstract
The first outbreak of red sea bream iridoviral disease (RSIVD) caused by red sea bream iridovirus (RSIV) was recorded among cultured red sea bream (Pagrus major) in 1990 in Ehime, Shikoku, Japan. Since then, the disease has caused mass mortalities of many cultured marine fishes. From 1990-2000, RSIVD was detected in 31 cultured marine fish species, including 28 Perciformes, 2 Pleuronectiformes and 1 Teteraodontiformes, in 18 prefectures in the southwestern part of Japan. The infected fish are lethargic and show severe anemia, petechiae of the gills, and enlargement of the spleen. Histopathologically, the disease is characterized by the presence of enlarged cells in the spleen, heart, kidney, liver and gills that are deeply stained with Giemsa solution.
Diagnostic methods for RSIV, such as the observation of stained imprints or tissue sections, an immunofluorescent (IF) test with a monoclonal antibody (MAb) and a polymerase chain reaction (PCR) technique have been developed. The IF test with MAb is commonly used in the rapid diagnosis of RSIV-infected fish. For an effective control measure against RSIVD, a formalin-killed vaccine has been developed and this showed a significant effect in red sea bream under both experimental and field conditions.
Citation
Nakajima, K. (2002). Diagnostic and preventive practices for iridovirus in marine fish. In Y. Inui & E. R. Cruz-Lacierda (Eds.), Disease Control in Fish and Shrimp Aquaculture in Southeast Asia - Diagnosis and Husbandry Techniques: Proceedings of the SEAFDEC-OIE Seminar-Workshop on Disease Control in Fish and Shrimp Aquaculture in Southeast Asia - Diagnosis and Husbandry Techniques, 4-6 December 2001, Iloilo City, Philippines (pp. 75-79). Tigbauan, Iloilo, Philippines: SEAFDEC Aquaculture Department.
Publisher
Aquaculture Department, Southeast Asian Fisheries Development CenterSubject
Type
Conference paperISBN
9718511601Related items
Showing items related by title, author, creator and subject.
-
Conference paper
Successful use of cryopreserved oyster trocophores as a live first feed larval marine fish and invertebrates
- In CL Marte, GF Quinitio & AC Emata (Eds.), Proceedings of the Seminar-Workshop on Breeding and Seed Production of Cultured Finfishes in the Philippines, Tigbauan, Iloilo, Philippines, 4-5 May 1993, 1996 - SEAFDEC Aquaculture DepartmentTrochophore-stage larvae of the Pacific oyster Crassostrea gigas were cryopreserved in bulk and stored in liquid nitrogen for periods up to two years before thawing and feeding to a variety of warmwater and coldwater larval marine fish, as well as to marine shrimps and other invertebrates. The commercial product ("TrochoFeed"), marketed in both pre-thawed and cryopreserved versions, has been used successfully in the early rearing of cultured species including red drum, snook, grouper, and black cod, as well as for numerous warmwater and coldwater aquarium display fish. This paper describes the nutritional profile of the cryopreserved trochopores and presents a summary of the available growth and mortality data. -
Article
Euryhaline rotifer Proales similis as initial live food for rearing fish with small mouth
-Aquaculture , 2014 - ElsevierThe SS-type rotifer Brachionus rotundiformis is a common initial food for rearing fish larvae with a small mouth. However, there are commercially important fish species whose mouth sizes are too small to feed on SS-type rotifers. In 2004, we isolated a small (body length = 82.7 ± 10.9 μm; body width 40.5 ± 6.4 μm), flexible, and iloricate rotifer, Proales similis from an estuary in Okinawa, Japan. Under laboratory conditions (25 °C, 2–25 ppt) P. similis produced its first offspring on 2.5 to 2.8 days after hatching, and produced 4.3 to 7.8 offspring within 4.0 to 4.7 days life span. Batch cultured P. similis fed Nannochloropsis oculata suspension at 28.8 μg dry weight ml− 1 and cultured at 25 °C, 25 ppt filtered seawater, increased exponentially from 25 to 2400 ind ml− 1 after 11 days of culture with an overall intrinsic rate of natural increase (r) of 0.42 day− 1. The growth rate of P. similis was not significantly different when fed fresh N. oculata and super fresh Chlorella vulgaris-V12®. Total lipid per wet weight of P. similis fed by N. oculata and C. vulgaris were 2.4 and 2.6%, respectively. The compositions of eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA), and arachidonic acid (ARA) of P. similis fed N. oculata were 23.2, 0.0 and 5.3%, respectively, while these were 11.0, 17.5 and 0.5% respectively, when fed C. vulgaris. The use of P. similis to feed small mouth fish including seven-band grouper Epinephelus septemfasciatus, rusty angelfish Centropyge ferrugata, and humphead wrasse Cheilinus undulatus showed that it is an excellent starter food for these species because of their high selectivity index and improved survival. In addition, P. similis was ingested by Japanese eel Anguilla japonica larvae with a complicated digestive system. The use of P. similis as starter feed for small mouth fish larvae is highly recommended. -
Conference paper
Community-based stock enhancement of abalone, Haliotis asinina in Sagay marine reserve: Achievements, limitations and directions
- In K Hajime, T Iwata, Y Theparoonrat, N Manajit & VT Sulit (Eds.), Consolidating the Strategies for Fishery Resources Enhancement in Southeast Asia. Proceedings of the Symposium … Strategy for Fisheries Resources Enhancement in the Southeast Asian Region, Pattaya, Thailand, 27-30 July 2015, 2016 - Training Department, Southeast Asian Fisheries Development CenterThe Sagay Marine Reserve (SMR) under the National Integrated Protected Area System (NIPAS) is one of the many reef areas in the Visayan Sea in the central part of the Philippine archipelago. The SMR covers 32,000 ha or 59% of coastal waters north of the mainland Sagay City. Donkey’s ear abalone is one of the most sought mollusks traded by small-scale fishers in Molocaboc Island located within the SMR. High buying prices in local and international markets compared with other fish catch motivated fishers to target abalone and caused its overfishing. SEAFDEC/AQD, with support from the Government of Japan Trust Fund (JTF), conducted a community-based stock enhancement through a tri-party collaboration between the fisherfolks of Molocaboc Island, the Sagay local government at the village and city levels, and SEAFDEC/AQD. The study showed that the decision and implementation of stock enhancement and the definition of its objectives and relevance involves the strong engagement with stakeholders. For over a period of eight years (2007-2014), we learned that stock enhancement necessarily involve high financial investments and enormous transaction cost over a long period of time which are often not affordable to local governments of coastal communities in Southeast Asia. Thus, community-based collaborations may help achieve enhancement and restocking goals.
