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Plantlet regeneration of Kappaphycus alvarezii var. adik-adik by tissue culture

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Date
2007
Author
Hurtado, A. Q.
Biter, A. B.
Page views
1,147
ASFA keyword
aquaculture ASFA
cell culture ASFA
colour ASFA
intensive aquaculture ASFA
light intensity ASFA
nursery grounds ASFA
plant morphology ASFA
taxonomy ASFA
tissue culture ASFA
AGROVOC keyword
Philippines AGROVOC
callus AGROVOC
cortex AGROVOC
explants AGROVOC
Filaments
Photons
Plantlets
Kappaphycus alvarezii AGROVOC
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Abstract
Three color morphotypes of Kappaphycus alvarezii var. adik-adik (brown, green and red) collected from a farming area in Tictauan Is., Zamboanga City, Philippines were used as explants in the study in order to micropropagate ‘new’ plants. Individual sections of sterile Kappaphycus alvarezii var. adik-adik, initially cultured in a 48-well culture plate containing ESS/2 + E3 + PGR, released callus cells after 4–5 days of incubation at 23–25°C, 13:11H LD cycle and 10–15 μmol photons m−2 s−1 light intensity. True calli were formed after 29–35 days following dense formation of filaments or undifferentiated round cells at the medullary and inner cortical layers of the section. Plantlets (2–3 mm long) of Kappaphycus alvarezii var. adik-adik were able to regenerate after 98, 150 and 177 days in-vitro among the reds, greens, and browns, respectively. This study established successful methods for the production and regeneration of tissue explants of Kappaphycus alvarezii var. adik-adik which can possibly be used to mass produce ‘new’ cultivars for land- and sea-based nurseries as sources for commercial farming.
URI
http://hdl.handle.net/10862/2059
Suggested Citation
Hurtado, A. Q., & Biter, A. B. (2007). Plantlet regeneration of Kappaphycus alvarezii var. adik-adik by tissue culture. Journal of Applied Phycology, 19(6), 783-786. https://doi.org/10.1007/s10811-007-9269-1 
DOI
10.1007/s10811-007-9269-1
Type
Article
ISSN
0921-8971; 1573-5176
Collections
  • Journal Articles [1201]

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