SEAFDEC/AQDINSTITUTIONAL REPOSITORY
    • English
    • 日本語
    • ไทย
    • Bahasa Indonesia
  • English 
    • English
    • 日本語
    • ไทย
    • Bahasa Indonesia
  • Login
View Item 
  •   SEAFDEC/AQD Institutional Repository Home
  • 03 SEAFDEC/AQD External Publications
  • Journal Articles, Conference Papers and Book Chapters by SEAFDEC Staff
  • Journal Articles
  • View Item
  •   SEAFDEC/AQD Institutional Repository Home
  • 03 SEAFDEC/AQD External Publications
  • Journal Articles, Conference Papers and Book Chapters by SEAFDEC Staff
  • Journal Articles
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

Screening of vibriosis in Asian seabass, Lates calcarifer using loop-mediated isothermal amplification (LAMP) assay

  • Global styles
  • MLA
  • Vancouver
  • Elsevier - Harvard
  • APA
  • Help
Thumbnail
Download URL
www.hvm.bioflux.com.ro
Date
2012
Author
Caipang, Christopher Marlowe A.
Pakingking , Rolando, Jr. ORCID
Apines-Amar, Mary Jane S.
Page views
1,711
Subject
vibriosis ASFA
aquatic animals ASFA
aquatic organisms ASFA
bio-assays ASFA
polymerase chain reaction ASFA
screening ASFA
detection ASFA
LAMP
sea bass AGROVOC
Lates calcarifer AGROVOC
diagnostic techniques AGROVOC
diagnosis AGROVOC
Taxonomic term
Vibrio harveyi GBIF
Lates calcarifer GBIF
Metadata
Show full item record

Share 
 
Abstract
The aim of this study was to standardize a loop-mediated isothermal amplification (LAMP) assay for the detection of Vibrio harveyi , the causative agent of vibriosis in Asian seabass, Lates calcarifer. The dnaJ gene of the bacterial pathogen was used as the target gene for the LAMP assay. It was optimized at an incubation time of 1 h at 63°C. The assay was highly specific for V. harveyi and did not cross-react with other bacterial pathogens offish. However, the assay was able to detect V. harveyi that was isolated from infected shrimps. The limit of detection of the LAMP assay was 40 pg of DNA mL-1 or 40 fg of the genomic DNA per LAMP reaction and was 10 times more sensitive than conventional PCR in detecting the bacterial pathogen from infected samples. The LAMP products can be quantified spectrophotometrically using hydroxynaphthol blue (HNB) dye and showed positive correlation with the amount of the pathogen. These results demonstrated that LAMP is a simple and sensitive detection technique that has potential application for routine diagnosis of vibrosis caused by V. harveyi in Asian seabass and other aquatic species.
URI
http://hdl.handle.net/10862/2172
Suggested Citation
Caipang, C. M. A., Pakingking , Jr., & Apines-Amar, M. J. S. (2012). Screening of vibriosis in Asian seabass, Lates calcarifer using loop-mediated isothermal amplification (LAMP) assay. Human & Veterinary Medicine, 4(2), 52-57. http://hdl.handle.net/10862/2172
Type
Article
ISSN
2066-7655; 2066-7663
Collections
  • Journal Articles [1158]

Related items

Showing items related by title, author, creator and subject.

  • Thumbnail

    Use of plant protein sources in aquafeed for high value marine species in ASEAN member states 

    Catacutan, Mae R. (Aquaculture Department, Southeast Asian Fisheries Development Center, 2015)
    Cultured marine aquatic species are predominantly carnivorous. Major species in the region are seabass, grouper, snapper, tiger shrimp, mangrove crab and abalone. These species, except for abalone, require a high level of ...
  • Thumbnail

    Nursery and grow-out culture of high-value fish species in sea cages 

    Gaitan, Albert G.; Toledo, Joebert D. (Aquaculture Department, Southeast Asian Fisheries Development Center, 2009)
  • Thumbnail

    Nursery culture of high-value fish species in brackishwater ponds 

    Ladja, Jocelyn M.; Gapasin, Rolando S. J. (Aquaculture Department, Southeast Asian Fisheries Development Center, 2009)

© SEAFDEC/AQD  2022
Contact Us | Send Feedback
 

 

Browse

All of SAIRCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects

My Account

LoginRegister

Statistics

View Usage Statistics

© SEAFDEC/AQD  2022
Contact Us | Send Feedback
 

 

Export citations

Export the current results of the search query as a citation list. Select one of the available citation styles, or add a new one using the "Citations format" option present in the "My account" section.

The list of citations that can be exported is limited to items.

Export citations

Export the current item as a citation. Select one of the available citation styles, or add a new one using the "Citations format" option present in the "My account" section.

Export Citations

EXTERNAL LINKS DISCLAIMER

This link is being provided as a convenience and for informational purposes only. SEAFDEC/AQD bears no responsibility for the accuracy, legality or content of the external site or for that of subsequent links. Contact the external site for answers to questions regarding its content.

If you come across any external links that don't work, we would be grateful if you could report them to the repository administrators.

Click DOWNLOAD to open/view the file. Request a copy in case the link we provided don't work.

Download

DOCUMENT REQUEST NOT AVAILABLE

This publication is still available (in PRINT) and for sale at AQD bookstore. The library is currently restricted to send PDF of publications that are still for sale.

You may contact bookstore@seafdec.org.ph or visit AQD bookstore for orders.

FILE UNDER EMBARGO

This file associated with this publication is currently under embargo. This will be available for download after the embargo date.