Screening of vibriosis in Asian seabass, Lates calcarifer using loop-mediated isothermal amplification (LAMP) assay
MetadataShow full item record
The aim of this study was to standardize a loop-mediated isothermal amplification (LAMP) assay for the detection of Vibrio harveyi , the causative agent of vibriosis in Asian seabass, Lates calcarifer. The dnaJ gene of the bacterial pathogen was used as the target gene for the LAMP assay. It was optimized at an incubation time of 1 h at 63°C. The assay was highly specific for V. harveyi and did not cross-react with other bacterial pathogens offish. However, the assay was able to detect V. harveyi that was isolated from infected shrimps. The limit of detection of the LAMP assay was 40 pg of DNA mL-1 or 40 fg of the genomic DNA per LAMP reaction and was 10 times more sensitive than conventional PCR in detecting the bacterial pathogen from infected samples. The LAMP products can be quantified spectrophotometrically using hydroxynaphthol blue (HNB) dye and showed positive correlation with the amount of the pathogen. These results demonstrated that LAMP is a simple and sensitive detection technique that has potential application for routine diagnosis of vibrosis caused by V. harveyi in Asian seabass and other aquatic species.
Caipang, C. M. A., Pakingking , Jr., & Apines-Amar, M. J. S. (2012). Screening of vibriosis in Asian seabass, Lates calcarifer using loop-mediated isothermal amplification (LAMP) assay.
Human & Veterinary Medicine, 4(2), 52-57. http://hdl.handle.net/10862/2172
- Journal Articles 
Showing items related by title, author, creator and subject.
Emata, Arnil C. (Aquaculture Department, Southeast Asian Fisheries Development Center, 1995)Most of the fish research at SEAFDEC AQD in 1992-1994 was on milkfish. Studies were conducted on year-round spawning through hormonal or environmental manipulation; optimum lipid and protein levels and ration size for ...
Catacutan, Mae R. (Aquaculture Department, Southeast Asian Fisheries Development Center, 2015)Cultured marine aquatic species are predominantly carnivorous. Major species in the region are seabass, grouper, snapper, tiger shrimp, mangrove crab and abalone. These species, except for abalone, require a high level of ...