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dc.contributor.authorCaipang, Christopher Marlowe A.
dc.contributor.authorPakingking Jr., Rolando V.
dc.contributor.authorApines-Amar, Mary Jane S.
dc.date.accessioned2015-04-09T13:51:44Z
dc.date.available2015-04-09T13:51:44Z
dc.date.issued2012
dc.identifier.citationCaipang, C. M. A., Pakingking Jr., R. V., & Apines-Amar, M. J. S. (2012). Screening of vibriosis in Asian seabass, Lates calcarifer using loop-mediated isothermal amplification (LAMP) assay. Human and Veterinary Medicine, 4(2), 52-57.en
dc.identifier.issn2066-7655
dc.identifier.urihttp://hdl.handle.net/10862/2172
dc.description.abstractThe aim of this study was to standardize a loop-mediated isothermal amplification (LAMP) assay for the detection of Vibrio harveyi , the causative agent of vibriosis in Asian seabass, Lates calcarifer. The dnaJ gene of the bacterial pathogen was used as the target gene for the LAMP assay. It was optimized at an incubation time of 1 h at 63°C. The assay was highly specific for V. harveyi and did not cross-react with other bacterial pathogens offish. However, the assay was able to detect V. harveyi that was isolated from infected shrimps. The limit of detection of the LAMP assay was 40 pg of DNA mL-1 or 40 fg of the genomic DNA per LAMP reaction and was 10 times more sensitive than conventional PCR in detecting the bacterial pathogen from infected samples. The LAMP products can be quantified spectrophotometrically using hydroxynaphthol blue (HNB) dye and showed positive correlation with the amount of the pathogen. These results demonstrated that LAMP is a simple and sensitive detection technique that has potential application for routine diagnosis of vibrosis caused by V. harveyi in Asian seabass and other aquatic species.en
dc.description.sponsorshipThe use of the facilities at the National Institute of Molecular Biology and Biotechnology (NIMBB), University of the Philippines Visayas and at the Fish Health Laboratory of the Southeast Asian Fisheries Development Center (SEAFDEC) is gratefully acknowledged.en
dc.language.isoenen
dc.publisherBioflux Societyen
dc.relation.urihttp://www.hvm.bioflux.com.ro/docs/2012.52-57.pdf
dc.subjectVibrio harveyien
dc.subjectLAMPen
dc.subjectSea bassen
dc.subjectLates calcariferen
dc.subjectDiagnostic techniquesen
dc.subjectDiagnosisen
dc.titleScreening of vibriosis in Asian seabass, Lates calcarifer using loop-mediated isothermal amplification (LAMP) assayen
dc.typeArticleen
dc.citation.volume4
dc.citation.issue2
dc.citation.spage52
dc.citation.epage57
dc.citation.journalTitleHuman & Veterinary Medicineen
dc.subject.asfaVibriosisen
dc.subject.asfaAquatic animalsen
dc.subject.asfaAquatic organismsen
dc.subject.asfaBioassaysen
dc.subject.asfaPolymerase chain reactionen
dc.subject.asfaScreeningen
dc.subject.asfaDetectionen
dc.identifier.essn2066-7663


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