Diet-tissue stable isotopic fractionation of tropical sea cucumber, Holothuria scabra
- Global styles
- APA
- Elsevier - Harvard
- Vancouver
- MLA
- Help

Associated URLs
www.jircas.affrc.go.jpDate
2013Author
Page views
880Metadata
Show full item recordCited times in Scopus
Share
Abstract
To provide a basis for a stable carbon and nitrogen isotope ratio (δ13C / δ15N) analysis to determine the assimilated organic matter in sea cucumber, Holothuria scabra, diet-tissue fractionations were experimentally determined by mono-feeding rearing with diatom. While δ15N fractionation of the whole body wall (2.4‰) was similar to the commonly accepted value (2.6 - 4‰), δ13C fractionation of the body wall (4.2‰) showed considerable discrepancy with the commonly accepted value (0 - 1‰) due to the high content (35% dry wt/wt) of calcareous spicules (CaCO3) in the body wall, which had significantly higher δ13C (-8.6‰) than the organic fractions. Computational elimination of spicules based upon spicule content and spicule δ13C reduced the δ13C fractionation of the body wall to 1.5‰, close to the common value. δ13C fractionation after spicule removal by acid decarbonation and subsequent rinsing (3.2‰) did not agree with the common value, and δ15N fractionation was significantly elevated by decarbonation. δ15N and δ13C fractionations of the intestine (1.5 and 2.2‰, respectively) did not agree with the common values. Since δ13C and δ15N of the feces did not differ significantly from those of the diet, feces may be used to determine ingested organic matter in the wild.
Suggested Citation
Watanabe, S., Kodama, M., Sumbing, J. G., & Lebata-Ramos, M. J. H. (2013). Diet-tissue stable isotopic fractionation of tropical sea cucumber, Holothuria scabra.Type
ArticleISSN
0021-3551Subject
Collections
- Journal Articles [1100]