Interactive effects of vitamin C and E supplementation on growth performance, fatty acid composition and reduction of oxidative stress in juvenile Japanese flounder Paralichthys olivaceus fed dietary oxidized fish oil
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2014Author
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Abstract
A study was conducted to determine the interactive effects of vitamin C (VC) and E (VE) supplementation on growth, fatty acid composition and oxidative status of Japanese flounder juveniles. Fish (initial average body weight of 1.1 ± 0.1 g) in triplicate were fed five test diets for 60 days. Control diet contained fresh fish oil (FFO, 8.9 meq/kg) with 100 mg α-tocopherol (α-Toc) equivalents/kg of VE and 500 mg ascorbic acid (AsA) equivalents/kg of VC (FFO100E/500C). The other four diets contained oxidized fish oil (OFO, 167.8 meq/kg) with varying levels of VE (mg/kg) and VC (mg/kg) (OFO100E/500C, OFO200E/500C, OFO100E/1000C and OFO200E/1000C). Fish fed FFO100E/500C and OFO100E/500C had no differences in body weight gain (BWG). However, fish fed OFO200E/1000C diet had a significantly lower BWG than FFO100E/500C. Fish fed OFO200E/500C and OFO100E/1000C showed no differences in thiobarbituric acid-reactive substance values compared with FFO100E/500C. Increasing the levels of VC and VE supplementation increased liver AsA and α-Toc contents, respectively. Liver α-Toc content was significantly increased with incremental dietary VC levels, indicating a sparing effect of VC on liver α-Toc content of fish. Increasing the levels of dietary VC and VE supplementations decreased concentrations of 20:5n-3, 22:5n-3 and 22:6n-3 in fish liver. Fish fed OFO100E/500C and OFO200E/1000C diets showed higher oxidative stress condition than those fed FFO100E/500C. In conclusion, dietary VC and VE supplementation could maintain normal growth and health condition of juvenile Japanese flounder fed OFO. However, high doses of both vitamin supplements induced fish lipid peroxidation under oxidative stress condition.
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Paralichthys olivaceus Vitamin C Vitamin E Lipid peroxidation Oxidative stress Fatty acid compositionSuggested Citation
Gao, J., Koshio, S., Ishikawa, M., Yokoyama, S., & Mamauag, R. E. (2014). Interactive effects of vitamin C and E supplementation on growth performance, fatty acid composition and reduction of oxidative stress in juvenile Japanese flounder Paralichthys olivaceus fed dietary oxidized fish oil. Aquaculture , 422–423, 84-90. https://doi.org/10.1016/j.aquaculture.2013.11.031
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Aya, Frolan; Moniruzzaman, Mohammad ; ; Won, Seonghun ; Hamidoghli, Ali ; ; (Springer, 2022-12-06)
This study examined the effects of feeding fermented tuna by-product (FTBP) on the growth, non-specific immune response, liver and intestinal morphology, and disease resistance of olive flounder Paralichthys olivaceus. Olive flounders (n = 20; 2 g) were randomly assigned into four dietary groups in triplicates. Fish were fed four test diets (50% crude protein; 10% crude lipid) for 10 weeks in which unprocessed tuna by-product (TBP) meal was replaced (on a protein basis) with 0% (FTBP0 as the control diet), 15% (FTBP15), 30% (FTBP30), and 60% (FTBP60) of FTBP protein. Results showed that growth performance, blood parameters, and proximate composition were not influenced by FTBP inclusion. Non-specific immune parameters such as superoxide dismutase activity in the FTBP30 and FTBP60 groups were significantly higher than in the FTBP15 and the control groups, whereas lysozyme and myeloperoxidase activities were not different. Liver histopathology revealed normal architecture in groups fed FTBP0 and FTBP15 diets although mild alterations were noted in the FTBP30- and FTBP60-fed groups. Intestinal villi height and muscular thickness were not significantly altered with FTBP inclusion. Moreover, higher cumulative survival rate was observed in the FTBP60-fed group than fish fed with the other diets following the 13-day challenge with E. tarda. Together, these results demonstrate that fermented tuna by-product meal could be included at 60% in olive flounder diets.
