Interactive effects of vitamin C and E supplementation on growth performance, fatty acid composition and reduction of oxidative stress in juvenile Japanese flounder Paralichthys olivaceus fed dietary oxidized fish oil
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A study was conducted to determine the interactive effects of vitamin C (VC) and E (VE) supplementation on growth, fatty acid composition and oxidative status of Japanese flounder juveniles. Fish (initial average body weight of 1.1 ± 0.1 g) in triplicate were fed five test diets for 60 days. Control diet contained fresh fish oil (FFO, 8.9 meq/kg) with 100 mg α-tocopherol (α-Toc) equivalents/kg of VE and 500 mg ascorbic acid (AsA) equivalents/kg of VC (FFO100E/500C). The other four diets contained oxidized fish oil (OFO, 167.8 meq/kg) with varying levels of VE (mg/kg) and VC (mg/kg) (OFO100E/500C, OFO200E/500C, OFO100E/1000C and OFO200E/1000C). Fish fed FFO100E/500C and OFO100E/500C had no differences in body weight gain (BWG). However, fish fed OFO200E/1000C diet had a significantly lower BWG than FFO100E/500C. Fish fed OFO200E/500C and OFO100E/1000C showed no differences in thiobarbituric acid-reactive substance values compared with FFO100E/500C. Increasing the levels of VC and VE supplementation increased liver AsA and α-Toc contents, respectively. Liver α-Toc content was significantly increased with incremental dietary VC levels, indicating a sparing effect of VC on liver α-Toc content of fish. Increasing the levels of dietary VC and VE supplementations decreased concentrations of 20:5n-3, 22:5n-3 and 22:6n-3 in fish liver. Fish fed OFO100E/500C and OFO200E/1000C diets showed higher oxidative stress condition than those fed FFO100E/500C. In conclusion, dietary VC and VE supplementation could maintain normal growth and health condition of juvenile Japanese flounder fed OFO. However, high doses of both vitamin supplements induced fish lipid peroxidation under oxidative stress condition.
CitationGao, J., Koshio, S., Ishikawa, M., Yokoyama, S., & Mamauag, R. E. P. (2014). Interactive effects of vitamin C and E supplementation on growth performance, fatty acid composition and reduction of oxidative stress in juvenile Japanese flounder Paralichthys olivaceus fed dietary oxidized fish oil.
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Growth and survival of grouper Epinephelus coioides (Hamilton) larvae fed free-living nematode Panagrellus redivivus at first feeding OS Reyes, MN Duray, CB Santiago & M Ricci -
Aquaculture International, 2011 - European Aquaculture SocietyThe free-living nematode, Panagrellus redivivus, was tested as live food for grouper Epinephelus coioides larvae during the first feeding stage. A series of experiments were conducted to determine the acceptability of the free-living nematodes in grouper larvae at first feeding, the optimum nematode density and the response of the larvae to nutritionally enriched nematode. All experiments were conducted in 200-L conical tanks filled with 150-L filtered seawater and stocked at 15 larvae L−1. Duration of feeding experiments was up to day 21 (experiment 1) and 14 days (experiment 2 and 3). Brachionus plicatilis and Artemia (experiment 1) and Brachionus plicatilis alone (experiment 2 & 3) was used as the control treatment. Observations indicated that the grouper larvae readily fed on free-living nematodes as early as 3 days posthatching, the start of exogenous feeding. Optimum feeding density for the larvae was 75 nematodes ml−1. The enrichment of cod liver oil or sunflower oil influenced the total lipids and n-3 highly unsaturated fatty acids of P. redivivus, which in turn influenced those of the grouper larvae, however, growth and survival of the larvae were not affected (P > 0.05). The results from this investigation showed that the nematode, P. redivivus, can be used as first live food for grouper larvae from the onset of exogenous feeding until they could feed on Artemia nauplii.
Book | Conference publication
Development and use of alternative ingredients or fish meal substitutes in aquaculture feed formulation: Proceedings of the ASEAN Regional Technical Consultation on Development and Use of Alternative Dietary Ingredients or Fish Meal Substitutes in Aquaculture Feed Formulation MR Catacutan, RM Coloso & BO Acosta (Eds.) - 2015 - Aquaculture Department, Southeast Asian Fisheries Development CenterRecognizing the need for a concerted effort to follow-up on this priority issue of the ASEAN on aquaculture feed development and utilization. SEAFDEC (Aquaculture Department and Secretariat) and the Government of Myanmar organized the 'Regional Technical Consultation (RTC) on development and Use of Alternative Dietary Ingredients or Fish Meal Substitutes in Aquaculture Feed Formulation'. The meeting was convened with the main purpose of providing a forum for charting the regional priorities and future directions on feed development, particularly on the use of alternative feed ingredients or protein substitutes. The specific objectives were to: (i) review the ASEAN-SEAFDEC member country status, constraints associated with developing alternative dietary ingredients for aquaculture feed; (ii) identify specific advances being made in the region with respect to the development of alternative aquaculture feed ingredients; and (iii) define approaches or initiatives supporting catch reduction of low-value/trash fish; (iv) formulate relevant policy recommendations (regional and country-specific) for effective development and utilization of aquaculture feeds; and (v) enhance cooperation among member countries and relevant stakeholders on initiatives that support sustainable aquaculture practices, particularly on feeds. This publication presents the outputs of the RTC. The country reports and review papers presented during the conference which are contained in this volume are cited individually.
Conference paperM Boonyaratpalin - In MR Catacutan, RM Coloso & BO Acosta (Eds.), Development and Use of Alternative Dietary Ingredients or Fish Meal Substitutes in Aquaculture Feed Formulation … Ingredients or Fish Meal Substitutes in Aquaculture Feed Formulation, 9-11 December 2014, Nay Pyi Taw, Myanmar, 2015 - Aquaculture Department, Southeast Asian Fisheries Development CenterFish meal (FM) is the best and main protein source for fish and shrimp feed because of the favorable amino acid profile, highly unsaturated fatty acids, palatability and absence of antinutritional factors. Aquaculture production has increased (about 8-9% a year) and is expected to increase further at the same rate to meet the demand for increase world population and health concern. In this connection, more aquafeed is required. Aquafeed rely much on fish meal (FM) and fish oil (FO) for the supply of major essential nutrients (essential amino acids, essential fatty acids, mineral and attractant). Therefore, more FM is required. However, world fish meal production has been relatively static in the last 15 years and is unlikely to increase further, coupled with public pressure on sustainable feed (fish in fish out ratio or fish meal independent ratio). Thus, levels of FM use in fish feed will have to be reduced and replaced by alternative protein sources for a sustainable increase in aquaculture production. Therefore, the identification and development of alternative feed ingredients that can replace FM is recognized as an international research priority. Consequently, several international integrated projects have been established in Europe, USA and Australia to reduce fish meal and fish oil in fish feeds. A European integrated project involving 14 countries, 32 partners with 4 major programs has been established to develop feeds from sustainable alternatives to fish meal and fish oil to produce safe, healthy seafood; assess the health benefits of fish farmed on the new diets; assess the safety of fish farmed on the new diets; assess perceptions regarding farmed fish and to devise a framework to communicate the risk and benefit of consuming farmed fish to the public and other stake holders. The strategic goal of this project is to tailor aquaculture feeds to produce high-quality fish with significantly reduced use of fish meal (FM) and fish oil (FO).