Development and characterization of nine novel microsatellite markers for the milkfish Chanos chanos
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The milkfish, Chanos chanos, is an important aquaculture resource in Southeast Asia. Using NGS data, 72 microsatellite markers were developed. PCR product confirmation using agarose gel electrophoresis allowed the identification of 24 utilizable markers. Milkfish samples (n = 48) from a hatchery stock in Palawan, Philippines were analyzed for genetic variability at the aforementioned 24 loci. Consequently, nine of these microsatellite loci were noted to have high success rate in amplification, exhibited polymorphism with 19 maximum number of alleles and no null alleles. These are the first microsatellite markers to be developed and characterized for C. chanos that will efficiently enable genetic stock delineation and monitoring as well as marker-aided genetic improvement research.
Suggested CitationSantos, B. S., Romana-Eguia, M. R. R., Basiao, Z. U., & Ikeda, M. (2015). Development and characterization of nine novel microsatellite markers for the milkfish Chanos chanos.
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Benitez, Lita V. (Philippine Council for Aquatic and Marine Research and Development, 1989)This paper reviews recent work on milkfish nutrition. Substantial progress had been made towards understanding the digestive physiology of milkfish. Major enzaymes envolved in the digestions of carbohydrates, protein and ...
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