Development and characterization of nine novel microsatellite markers for the milkfish Chanos chanos
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Abstract
The milkfish, Chanos chanos, is an important aquaculture resource in Southeast Asia. Using NGS data, 72 microsatellite markers were developed. PCR product confirmation using agarose gel electrophoresis allowed the identification of 24 utilizable markers. Milkfish samples (n = 48) from a hatchery stock in Palawan, Philippines were analyzed for genetic variability at the aforementioned 24 loci. Consequently, nine of these microsatellite loci were noted to have high success rate in amplification, exhibited polymorphism with 19 maximum number of alleles and no null alleles. These are the first microsatellite markers to be developed and characterized for C. chanos that will efficiently enable genetic stock delineation and monitoring as well as marker-aided genetic improvement research.
Suggested Citation
Santos, B. S., Romana-Eguia, M. R. R., Basiao, Z. U., & Ikeda, M. (2015). Development and characterization of nine novel microsatellite markers for the milkfish Chanos chanos. Conservation Genetics Resources , 7(2), 451-453. https://doi.org/10.1007/s12686-014-0393-3
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ArticleISSN
1877-7252; 1877-7260Collections
- Journal Articles [1266]
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Evaluation of organic and inorganic fertilizers in brackishwater milkfish ponds
(Elsevier, 1989)The study was conducted in twelve 144-m2 ponds to evaluate the effect of different organic and inorganic fertilizers on the growth, survival, gross production, and profitability of marketable milkfish. The following treatments were used: Treatment I, SEAFDEC traditional fertilization practice (16-20-0 at 50 kg/ha and 45-0-0 at 15 kg/ha); Treatment II, half-dosage of Treatment I; Treatment III, chicken manure at 0.5 ton/ha; and Treatment IV, MASA (processed from agricultural and industrial wastes) fertilizer at 0.5 ton/ha. All treatments were applied once in every 2 weeks. No significant difference (P > 0.05) existed in the harvest and production of milkfish among the treatments. However, economic indicators such as return-on-investment (ROI), payback period, and marginal analysis ranked the performance of the fertilizer treatments in the order of I, II, III and IV. Fish kills occurred in three ponds applied with chicken manure and MASA fertilizer. This could have been due to a heavy build-up of organic matter in the pond bottom which led to the collapse of the benthic algal community, depletion of dissolved oxygen and the presence of hydrogen sulfide. It is therefore suggested that a lower dosage of organic fertilizer should be applied in ponds especially during the rainy season. -
Milkfish breeding and hatchery technology at SEAFDEC/AQD
(Aquaculture Department, Southeast Asian Fisheries Development Center, 1999)Describes the techniques already adopted by the private sector: broodstock management, broodstock diet, commercial fry production, live transport, and larval diet. A list of AQD research publications on milkfish is included.
