Development and characterization of nine novel microsatellite markers for the milkfish Chanos chanos
View/
Date
2015
Page views
2,177AGROVOC keyword
Taxonomic term
Metadata
Show full item recordCited times in Scopus
Share
Abstract
The milkfish, Chanos chanos, is an important aquaculture resource in Southeast Asia. Using NGS data, 72 microsatellite markers were developed. PCR product confirmation using agarose gel electrophoresis allowed the identification of 24 utilizable markers. Milkfish samples (n = 48) from a hatchery stock in Palawan, Philippines were analyzed for genetic variability at the aforementioned 24 loci. Consequently, nine of these microsatellite loci were noted to have high success rate in amplification, exhibited polymorphism with 19 maximum number of alleles and no null alleles. These are the first microsatellite markers to be developed and characterized for C. chanos that will efficiently enable genetic stock delineation and monitoring as well as marker-aided genetic improvement research.
Suggested Citation
Santos, B. S., Romana-Eguia, M. R. R., Basiao, Z. U., & Ikeda, M. (2015). Development and characterization of nine novel microsatellite markers for the milkfish Chanos chanos. Conservation Genetics Resources , 7(2), 451-453. https://doi.org/10.1007/s12686-014-0393-3
Type
ArticleISSN
1877-7252; 1877-7260Collections
- Journal Articles [1201]
Related items
Showing items related by title, author, creator and subject.
-
Series: Aquaculture extension manual; No. 63
Seed production of milkfish Chanos chanos Forsskal
(Aquaculture Department, Southeast Asian Fisheries Development Center, 2016)A 26-page manual describing the site selection, hatchery design, spawning, larval rearing, natural food production, and economic analysis for milkfish. -
Ration reduction, integrated multitrophic aquaculture (milkfish-seaweed-sea cucumber) and value-added products to improve incomes and reduce the ecological footprint of milkfish culture in the Philippines
(AquaFish Collaborative Research Support Program, Oregon State University, 2012)In the Philippines, cage culture of milkfish in marine environments is increasing. The practice uses high stocking densities, with significantly greater inputs of artificial feeds which more often than not, have led to ... -
Annual report 1979
(Aquaculture Department, Southeast Asian Fisheries Development Center, 1980)The annual report for 1979 is presented. A summary of the centre's activities is given with particular emphasis on the milkfish, Chanos chanos, programme and the prawn programme. Administrative details are included.
