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dc.contributor.authorSantos, Brian S.
dc.contributor.authorRomana-Eguia, Maria Rowena R.
dc.contributor.authorBasiao, Zubaida U.
dc.contributor.authorIkeda, Minoru
dc.identifier.citationSantos, B. S., Romana-Eguia, M. R. R., Basiao, Z. U., & Ikeda, M. (2015). Development and characterization of nine novel microsatellite markers for the milkfish Chanos chanos. Conservation Genetics Resources, 7(2), 451–453.en
dc.description.abstractThe milkfish, Chanos chanos, is an important aquaculture resource in Southeast Asia. Using NGS data, 72 microsatellite markers were developed. PCR product confirmation using agarose gel electrophoresis allowed the identification of 24 utilizable markers. Milkfish samples (n = 48) from a hatchery stock in Palawan, Philippines were analyzed for genetic variability at the aforementioned 24 loci. Consequently, nine of these microsatellite loci were noted to have high success rate in amplification, exhibited polymorphism with 19 maximum number of alleles and no null alleles. These are the first microsatellite markers to be developed and characterized for C. chanos that will efficiently enable genetic stock delineation and monitoring as well as marker-aided genetic improvement research.en
dc.description.sponsorshipFunds for this study were provided by the Department of Science and Technology-Philippine Council for Agriculture, Aquatic and Natural Resources Research and Development (DOST-PCAARRD) and the Southeast Asian Fisheries Development Center, Aquaculture Department (SEAFDEC/AQD; study code Br-02-F2012B).en
dc.publisherSpringer Verlagen
dc.subjectMicrosatellite markers
dc.subjectChanos chanos
dc.subjectPopulation genetic structure
dc.titleDevelopment and characterization of nine novel microsatellite markers for the milkfish Chanos chanosen
dc.citation.journalTitleConservation Genetics Resourcesen

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