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Development and characterization of nine novel microsatellite markers for the milkfish Chanos chanos

dc.contributor.authorSantos, Brian S.
dc.contributor.authorRomana-Eguia, Maria Rowena R.
dc.contributor.authorBasiao, Zubaida U.
dc.contributor.authorIkeda, Minoru
dc.date.accessioned2015-06-01T03:45:38Z
dc.date.available2015-06-01T03:45:38Z
dc.date.issued2015
dc.identifier.citationSantos, B. S., Romana-Eguia, M. R. R., Basiao, Z. U., & Ikeda, M. (2015). Development and characterization of nine novel microsatellite markers for the milkfish Chanos chanos. Conservation Genetics Resources, 7(2), 451–453.en
dc.identifier.issn1877-7252
dc.identifier.urihttp://hdl.handle.net/10862/2266
dc.description.abstractThe milkfish, Chanos chanos, is an important aquaculture resource in Southeast Asia. Using NGS data, 72 microsatellite markers were developed. PCR product confirmation using agarose gel electrophoresis allowed the identification of 24 utilizable markers. Milkfish samples (n = 48) from a hatchery stock in Palawan, Philippines were analyzed for genetic variability at the aforementioned 24 loci. Consequently, nine of these microsatellite loci were noted to have high success rate in amplification, exhibited polymorphism with 19 maximum number of alleles and no null alleles. These are the first microsatellite markers to be developed and characterized for C. chanos that will efficiently enable genetic stock delineation and monitoring as well as marker-aided genetic improvement research.en
dc.description.sponsorshipFunds for this study were provided by the Department of Science and Technology-Philippine Council for Agriculture, Aquatic and Natural Resources Research and Development (DOST-PCAARRD) and the Southeast Asian Fisheries Development Center, Aquaculture Department (SEAFDEC/AQD; study code Br-02-F2012B).en
dc.language.isoenen
dc.publisherSpringer Verlagen
dc.subjectMicrosatellite markers
dc.subjectmilkfishen
dc.subjectChanos chanosen
dc.subjectPopulation genetic structure
dc.titleDevelopment and characterization of nine novel microsatellite markers for the milkfish Chanos chanosen
dc.typeArticleen
dc.identifier.doi10.1007/s12686-014-0393-3
dc.citation.volume7
dc.citation.issue2
dc.citation.spage451
dc.citation.epage453
dc.citation.journalTitleConservation Genetics Resourcesen
dc.identifier.essn1877-7260
dc.subject.scientificNameChanos chanos


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  • Journal Articles [1229]
    These papers were contributed by Department staff to various national and international journals.

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