Changes in the gastrointestinal tract and associated organs during early development of the grouper (Epinephelus coioides)
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The histomorphological changes in the gastrointestinal tract of Epinephelus coioides and associated organs during its early development were studied. Larvae of E. coioides were reared in 5-tonne tanks using the semi-intensive culture system. Larval samples were collected at days 0, 2, 4, 6, 8, 10, 12, 14, 16, 20, 25, 30, 35, 40, 45 and 60. The total length (TL) of about 10-20 larvae per sampling was measured. At least 3 samples were examined from each stage for longitudinal sections using light microscopy. The digestive tract of day 0 larvae was a straight, undifferentiated tube composed of simple cuboidal cells. At day 2, cellular differentiation was observed in the pharynx, oesophagus, primordial stomach and intestine. The primordial stomach broadened into a voluminous pouch at day 10. The gastric gland was observed in the stomach from day 20. Day 35 seemed to be the proper time to feed larvae with minced fish when using the semi-intensive rearing system. Insignificant histomorphological changes in the metamorphosing grouper larvae were observed from days 40-60.
Quinitio, G. F., Sa-an, A. C., Toledo, J. D., & Tan-Fermin, J. D. (2004). Changes in the gastrointestinal tract and associated organs during early development of the grouper (Epinephelus coioides). In M. A. Rimmer, S. McBride, & K. C. Williams (Eds.), Advances in grouper aquaculture (pp. 26–29). Canberra: Australian Centre for International Agricultural Research.
SeriesACIAR Monograph 110
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