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Dietary lipid requirement of whiteleg shrimp Litopenaeus vannamei juveniles cultured in biofloc system
(Wiley, 2020-06)
An eight-week feeding trial was conducted to evaluate the contribution of biofloc on dietary lipid requirement in whiteleg shrimp Litopenaeus vannamei. Five diets with graded levels of dietary lipid (45, 60, 90, 120 and ...
Determination of poly‐β‐hydroxybutyrate assimilation by postlarval whiteleg shrimp, Litopenaeus vannamei using stable 13C isotope tracing
(Wiley, 2021-02)
A preliminary study was conducted to demonstrate fate of the bacterial storage compound poly-β-hydroxybutyrate (PHB) once ingested and degraded in vivo in crustaceans. The 2% supplementation of <sup>13</sup>C-labeled ...
An innovative biofloc technology for the nursery production of Pacific whiteleg shrimp, Penaeus vannamei in tanks
(International Network for Natural Sciences, 2022-10-09)
Nursery production of shrimp is usually done in small ponds; however, the use of small and circular tanks with plastic liners is gaining popularity. From an industry standpoint, there is still a need to assess how nursery systems can be of benefit to the shrimp production cycle. Hence, the use of small circular tanks coupled with the incorporation of biofloc technology was assessed in terms of its viability during the nursery production of the Pacific whiteleg shrimp, Penaeus vannamei. A 450m<sup>2</sup> plastic lined circular tank was installed and prepared for the stocking of P. vannamei postlarvae (PLs) at a density of 500 PLs per m<sup>2</sup>. Biofloc was produced and maintained throughout the nursery phase using brown sugar as carbon source at a carbon to nitrogen (C:N) ratio of 10. Water quality was monitored daily, while presumptive Vibrios were enumerated weekly. Sampling for growth was done at the 14<sup>th</sup> day post-stocking and weekly until harvest on the 30th day. The different water quality parameters were within optimum levels required for shrimp growth. Presumptive Vibrios were dominated by the yellow colonies. At the end of the nursery phase, there was 100% survival and the shrimp attained an average body weight of 1.26 g and a feed conversion ratio (FCR) of 0.43. Our results indicate that the use of small circular tanks with biofloc during the nursery production phase of whiteleg shrimp is feasible and can be incorporated in the grow-out culture of this shrimp species....
Biofloc-based nursery production system: Heeding the call towards a sustainable shrimp culture industry in the Philippines
(International Network for Natural Sciences, 2022)
The increasing global population resulted in intense pressure on the food production sectors to meet the rise in food demand. The aquaculture industry, which is one of the major food production sectors, provides opportunities ...
Vibrio load and percentage composition of sucrose‐fermenting vibrios in Nile tilapia (Oreochromis niloticus) cultured in brackish water earthen ponds and biocontrol potential of recycled tilapia‐conditioned greenwater against population growth of V. parahaemolyticus and non‐sucrose‐fermenting vibrios in the grow‐out culture of white leg shrimp (Litopenaeus vannamei)
(Wiley, 2022-10-20)
<i>Vibrio</i> are known opportunistic pathogens of pond-cultured fish and shrimp. This study determined the presumptive <i>Vibrio</i> count (PVC) and percentage composition of “beneficial” sucrose-fermenting vibrios (SFVs) ...
Lime application to condition soil to pH 11 controls growth of harmful vibrios and WSSV
(European Association of Fish Pathologists, 2023-05-26)
Shrimp farming has been devastated by several diseases. Disease outbreaks can be prevented by optimising environmental conditions or by eliminating risks factors including carrier organisms. These can be achieved through proper pond preparation. One important step in pond preparation is the application of hydrated lime.
This paper describes the effect of hydrated lime application to condition pond soil to pH 11 on the pond ecosystem, more specifically on soil pH and biota. The experiment was done using three 600 m<sup>2</sup> brackish water ponds. After hydrated lime application, the wet soil pH on the 1<sup>st</sup>-8<sup>th</sup> day was significantly higher (P<0.05) compared to the pH before liming to pH 11, and flushing after flooding for 24 h. Soil pH of 11 was observed 24-48h after lime application. Moreover, unwanted species like crabs and fish were found dead. Liming to pH 11 negatively affects the counts of green vibrio colonies thereby decreasing the percentage of green vibrios (PGV). In another experiment, three 750m<sup>2</sup> earthen ponds were used. The pond soils were previously positive for the Whitespot Syndrome Virus (WSSV), hydrated lime were applied to bring the pond soil pH to 11. After lime application, WSSV was not detected in the pond soils.
Results showed that increasing the soil pH to ≥11 positively affects the pond ecosystem. Liming encourages the proliferation of yellow vibrios and the eradication of carrier species (i.e. crabs) as well as predators (i.e. fish) in the ponds. Furthermore, liming to pH 11 may also eradicate White spot virus from the pond sediment. To implement a 1-unit increase in wet pH, hydrated lime should be applied at 2T/ha....
Complete genome sequence of Vibrio parahaemolyticus strain PH1273, isolated from aquacultured shrimp in the Philippines
(American Society for Microbiology, 2023-10-19)
We announce the complete genome sequence of Vibrio parahaemolyticus strain PH1273. This strain was collected from a Penaeus vannamei pond in the Philippines in 2015. Genome analysis revealed that it lacks the gene pirAB ...