Marker-aided genetic stock management: prospects in Philippine aquatic biodiversity conservation and aquaculture
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With the advent of DNA marker-based technologies and applications, genetic stock assessment incorporating molecular marker information has become an important tool in managing resources both for aquaculture and stock enhancement. Local initiatives toward this end have been undertaken by several research and academic agencies particularly those with access to advanced molecular genetic laboratory facilities both in the Philippines and in collaborating foreign institutions. Funds coming from the Philippine Department of Science and Technology and/or international research grants have supported work on commercially valuable species such as tilapia, shrimp, mud crabs, abalone, milkfish and some high value marine fishes with a view of utilizing and in the process, demonstrating the significance of more scientific microlevel assessment of stocks. Information drawn from marker-aided genetic stock evaluation can contribute to a better understanding of the impact of how proper stock management can be more effectively achieved and how this method can gradually translate to improved yields both from culture and fisheries. This paper covers a review of the status of this technology as applied to ongoing fish conservation and aquaculture production efforts in the Philippines.
Romana-Eguia, M. R. R., Ikeda, M., & Kijima, A. (2015). Marker-aided genetic stock management: prospects in Philippine aquatic biodiversity conservation and aquaculture. In M. R. R. Romana-Eguia, F. D. Parado-Estepa, N. D. Salayo, & M. J. H. Lebata-Ramos (Eds.), Resource Enhancement and Sustainable Aquaculture Practices in Southeast Asia: Challenges in Responsible Production of Aquatic Species: Proceedings of the International Workshop on Resource Enhancement and Sustainable Aquaculture Practices in Southeast Asia 2014 (RESA) (pp. 213-222). Tigbauan, Iloilo, Philippines: Aquaculture Dept., Southeast Asian Fisheries Development Center. http://hdl.handle.net/10862/2776
PublisherAquaculture Department, Southeast Asian Fisheries Development Center
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Conference paperMRR Romana-Eguia - In JH Primavera, ET Quinitio & MR Eguia (Eds.), Proceedings of the Regional Technical Consultation on Stock Enhancement for Threatened Species of International Concern, Iloilo City, Philippines, 13-15 July 2005, 2006 - Aquaculture Department, Southeast Asian Fisheries Development CenterAquaculture and fisheries management require tools for identifying individuals or groups of aquatic organisms for the purpose of monitoring performance (growth, survival and behavior) and stock structure. In aquaculture research, commercially important traits of tagged individuals are assessed to generate supportive data for selective breeding, genetic improvement and commercial-scale fish farming. Fisheries management employs identification systems for the evaluation of stock abundance, population dynamics and documentation of wild and hatchery-bred stocks. Stock structure analysis is useful in the planning and implementation of sound stock management and more importantly, in stock enhancement programs. Blankenship and Leber (1995) underscored the inclusion of tagging/marking strategies for released hatchery stocks in the guidelines for responsible marine stock enhancement. Identifying and keeping track of introduced stocks in release habitats allows an assessment of their adaptability in the wild (Allendorf et al., 1988) and the success of the reseeding and/or restocking effort. Although often used interchangeably, the terms ‘tags’ and ‘markers’ differ by definition. Tags are artificial or synthetic materials that are attached to the aquatic organism to allow individual or group identification while markers are traits or characters either applied or inherent to the organism (Thorsteinsson, 2002). Tags/markers are essential in evaluating resource distribution patterns, behavior, migration and movement of stocks, dynamics of exploited aquatic populations and evolutionary processes, all of which comprise baseline information for any stock management, enhancement and conservation program in aquaculture and fisheries (Allendorf et al 1988, Mulvey et al., 1998).
Genetic assessment of milkfish (Chanos chanos Forsskal) stocks based on novel short tandem repeats for marker-aided broodstock management MRR Romana-Eguia, BS Santos, M Ikeda, ZU Basiao & A Kijima -
Aquaculture Research, 2018 - John Wiley and SonsMilkfish hatchery broodstock are either from on-grown wild-caught or hatchery-produced fry/juveniles. To determine if a marker-assisted management scheme can be formulated for improved milkfish hatchery production, milkfish stocks were genetically characterized using nine novel short tandem repeats or microsatellites. Eight wild-bred Philippine stocks (CLA, CUR, CAM, SIH, SBH-I1, HH, PAL and ZH-P0), four hatchery-bred stocks (SBH-I2, SBH-D, BoH and ZH-F1), two farm stocks of known mixed lineages (SPH and BDH) and one Indonesian hatchery-bred stock (WJH) were assessed. WJH was included since milkfish fingerlings from Indonesia reared in Philippine farms could be developed into future broodstock. Mean allelic richness (Ar) was highest in wild-bred stocks (9.5) and lowest in hatchery-bred spawners (9.1). Mean expected heterozygosities (He) were relatively similar in all stocks with wild-bred stocks slightly higher (0.67) than the others. An analysis of molecular variance indicated significant yet low genetic differentiation among stocks (FST = 0.013; p = .000) where variation (98.6%) was explained by intra-stock differences. In some of the domesticated stocks, reductions in mean allelic richness were observed in first generation hatchery broodstock (e.g. ZH-F1; Ar = 8.3), compared with their founder stock (e.g. ZH-P0; Ar = 9.4). The Indonesian stock was similar to local wild-bred stocks based on genetic variability indices; thus, it might be likely that the local stocks’ fitness traits could be comparable with the imported milkfish stock which has been perceived to be better. The quality of locally available farmed milkfish and prospects of formulating a broodstock management scheme for the production of good quality milkfish seedstock are herewith discussed.
magazineArticleDetermining changes in the genetic diversity of selected hatchery stocks through DNA-level polymorphisms analysis provides aquaculturists with a means to monitor inbreeding, control loss of genetic diversity, and achieve sustainable levels of genetic gain in the development of improved stocks. Tests with selected and domesticated tilapia stocks in the Philippines revealed variability between marker system results.