Marker-aided genetic stock management: prospects in Philippine aquatic biodiversity conservation and aquaculture
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Abstract
With the advent of DNA marker-based technologies and applications, genetic stock assessment incorporating molecular marker information has become an important tool in managing resources both for aquaculture and stock enhancement. Local initiatives toward this end have been undertaken by several research and academic agencies particularly those with access to advanced molecular genetic laboratory facilities both in the Philippines and in collaborating foreign institutions. Funds coming from the Philippine Department of Science and Technology and/or international research grants have supported work on commercially valuable species such as tilapia, shrimp, mud crabs, abalone, milkfish and some high value marine fishes with a view of utilizing and in the process, demonstrating the significance of more scientific microlevel assessment of stocks. Information drawn from marker-aided genetic stock evaluation can contribute to a better understanding of the impact of how proper stock management can be more effectively achieved and how this method can gradually translate to improved yields both from culture and fisheries. This paper covers a review of the status of this technology as applied to ongoing fish conservation and aquaculture production efforts in the Philippines.
Suggested Citation
Romana-Eguia, M. R. R., Ikeda, M., & Kijima, A. (2015). Marker-aided genetic stock management: prospects in Philippine aquatic biodiversity conservation and aquaculture. In M. R. R. Romana-Eguia, F. D. Parado-Estepa, N. D. Salayo, & M. J. H. Lebata-Ramos (Eds.), Resource Enhancement and Sustainable Aquaculture Practices in Southeast Asia: Challenges in Responsible Production of Aquatic Species: Proceedings of the International Workshop on Resource Enhancement and Sustainable Aquaculture Practices in Southeast Asia 2014 (RESA) (pp. 213-222). Tigbauan, Iloilo, Philippines: Aquaculture Dept., Southeast Asian Fisheries Development Center.
Type
Conference paperISBN
9789719931041
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Genetic assessment of philippine milkfish (Chanos chanos) stocks based on novel microsatellites for markeraided broodstock management
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Reports on genetic diversity within/among milkfish populations using DNA markers are sparse. Earlier work dealt with evolutionary relationships among wild populations to define management units in the Indo-Pacific region where milkfish naturally abound. However, application of milkfish genetic markers in broodstock development and management remains to be investigated. Here, nine novel microsatellites developed by NGS were utilized to (a) describe the genetic structure of Philippine wild and hatchery-bred milkfish; (b) monitor the impact of domestication selection and inbreeding; and (c) formulate marker-aided broodstock management methods, a prerequisite to genetic improvement. Milkfish samples from three wild populations: Claveria (CLA), Currimao (CUR), Camiguin (CAM); eleven local hatchery stocks: SEAFDEC Integrated Hatchery (SIH), SEAFDEC Big Hatchery-Igang batches 1 and 2 (SBH-I1 and SBH-I2), SEAFDEC Big Hatchery-Dumangas (SBH-D), Hautea Hatchery (HH), Sual Pangasinan Hatchery (SPH), BFAR Dagupan Hatchery (BDH), BFAR Bohol Hatchery (BoH), BFAR Palawan Hatchery (PAL), Zambales Hatchery-P0 (ZH-P0), Zambales Hatchery-F1 (ZH-F1); and a hatchery stock from West Java in Indonesia (WJH), were examined. An Indonesian stock was included since in the Philippines, Indonesian milkfish fingerlings are imported and farmed for having purportedly better production traits. Genetic diversity indices such as expected heterozygosity (He) and allele frequency (A) ranged from 0.655 to 0.697 and 9.2 to 11.1, respectively. AMOVA showed significant but low genetic differentiation among the milkfish populations (FST = 0.013; P=0.000,) since much of the variation is attributed to intrapopulation differences (98.6%). The oldest hatchery stock SIH (30-35 years) had relatively moderate genetic variability (He = 0.66, A = 10.6), which is lower than that of 5-year old SBH-I1 (He= 0.687, A= 11.5) considering that both stocks originally came from the same source in the wild. A reduction in genetic diversity was seen when a local hatchery stock (ZH-P0;He = 0.66 and A= 10.8) was monitored after one generation (ZH-F1; He = 0.65, A=9.3). Finally, the Indonesian stock WJH had genetic variability levels (He= 0.66; A= 10.5) comparable with local stocks. Results of genetic analyses are herein discussed in the context of promoting effective milkfish broodstock management practices for the production of good quality seed stock. -
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