Utilization of mung bean, Vigna radiata (Linnaeus) as a novel protein source in practical-type diets for juvenile milkfish, Chanos chanos (Forsskal): Effects on growth, feed efficiency, body composition, and histology of gut and liver
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A 15-week feeding trial was conducted to determine the optimum partial inclusion of mung bean protein in milkfish diet. Six isonitrogenous practical-type diets with mung bean included at 0%, 4%, 8%, 12%, 16%, and 20% of the diet equivalent to 0%, 3%, 7%, 10%, 13%, and 17% of the total dietary protein, respectively, were formulated. Milkfish with average body weight (ABW) of 8.5 ± 0.23g were distributed in eighteen tanks (6 treatments X 3 replications) with 10 fish each. The fish were fed the diets three times daily. Results showed that growth of milkfish was not adversely affected by the inclusion of mung bean protein at any dietary level. Feed conversion ratio (FCR) and protein efficiency ratio (PER) were significantly improved by the inclusion of mung bean at 20% of the diet. Nutrient compositions of the fish carcass were similar in all diets. Furthermore, no detrimental effects attributable to mung bean inclusion were seen in terms of protein retention, hepatosomatic index (HSI), and liver and midgut histology of the fish. Overall, mung bean is a promising protein source for milkfish and can be included up to 20% of the diet contributing as much as 17% of the total dietary protein without detrimental effects on growth, feed performance, PER, protein retention, HSI, and liver and intestinal histology.
CitationApines-Amar, M. J. S., Coloso, R. M., Amar, M. N. G., Golez, M. S. M., Bunda, M. G. B., & Jaspe, C. J. (2015). Utilization of mung bean, Vigna radiata (Linnaeus) as a novel protein source in practical-type diets for juvenile milkfish, Chanos chanos (Forsskal): Effects on growth, feed efficiency, body composition, and histology of gut and liver.
PublisherSociety of Israeli Aquaculture and Marine Biotechnology (SIAMB)
This study was funded by the Department of Science and Technology (DOST) through the Philippine Council for Agriculture, Aquatic, and Natural Resources Research and Development (PCAARRD).
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Conference paperAC Emata - In TU Bagarinao & EEC Flores (Eds.), Towards sustainable aquaculture in Southeast Asia and Japan: Proceedings of the Seminar-Workshop on Aquaculture Development in Southeast Asia, Iloilo City, Philippines, 26-28 July, 1994, 1995 - SEAFDEC Aquaculture DepartmentMost of the fish research at SEAFDEC AQD in 1992-1994 was on milkfish. Studies were conducted on year-round spawning through hormonal or environmental manipulation; optimum lipid and protein levels and ration size for captive broodstock; and the influence of spawner age on reproductive performance. The economics of hatchery operations, alone or integrated with broodstock as a commercial enterprise, was assessed. Mass production of larvae was refined with the use of commercial or SEAFDEC-formulated larval diets. Alternative rearing schemes in large tanks and ponds were tried. Hatcheryproduced and wild-caught larvae were compared in terms of growth and production in experimental nursery and grow-out ponds. Supplemental diets for brackishwater grow-out culture were formulated. Studies on broodstock management of grouper Epinephelus spp. included lipid enrichment of the diet and hormonal induction of sex inversion. Seed production techniques were developed but survival rates were low. Grouper culture was found economically feasible in experimental ponds with 'trash' fish as feed. The mangrove red snapper Lutjanus argentimaculatus was successfully induced to spawn with injection of human chorionic gonadotropin. Initial larval rearing trials were successful but survival rates must be improved. Hormonal manipulation of spawning of the Asian sea bass Lates calcarifer allows seed production during most of the year. Photoperiod manipulation leads to maturation of females, but not males, beyond the natural breeding season (April-November). Nursery rearing of 9 mm juveniles is feasible in floating net cages with night lights that attract food zooplankton. The requirements of sea bass for lipid, protein, carbohydrates, and essential amino acids were determined. In the rabbitfish Siganus guttatus, weekly injections of luteinizing hormone releasing hormone analogue (LHRHa) sustains milt production for three weeks. Thyroid hormones injected into broodstocks improved the growth of larvae to day 7. Induced spawning techniques for the Asian catfish Clarias macrocephalus were refined by determining the seasonal responsiveness to LHRHa and pimozide injections and testing for pheromonal induction of spontaneous spawning. The optimum insemination rate was determined and egg hatchability was enhanced by removal of the adhesive coat before incubation. Several practical diets for catfish during grow-out culture were tested against 'trash' fish. The broodstock management for bighead carp Aristichthys nobilis was studied. Cage-reared juveniles from cage-reared broodstock showed the best growth. To improve the reproductive performance, the broodstock diets were supplemented with vitamins A, C, and E. Research on tilapias focused on genetics and strain selection. Several strain testing procedures for Nile tilapia were evaluated in their efficiency to detect economically important strain differences. Reference lines were developed from two existing red tilapia strains to measure and reduce the effects of uncontrolled nongenetic variables in strain evaluation experiments with Nile tilapia. The tolerance of two Nile tilapia strains to heavy metals was similar when gauged by the 24-hour and 96-hour lethal concentration and by fish growth, survival, and reproductive performance. In a separate study, four strains of red tilapia showed generally higher seed production when reared in tanks than in cages. Improvements in the feed and feeding management for Nile tilapia were also studied. Intensive tilapia farming and feeding have led to oxygen depletion and fish kills in Sampaloc Lake. To rehabilitate the lake, it is imperative to reduce the farming area from 30 to 6 hectares; stop the use of commercial feeds; and remove the water hyacinths and other debris. Fish kills in Laguna de Bay have also become serious in recent years, and a review of the occurrences, losses, and possible causes is currently being conducted. Studies on the epizootic ulcerative syndrome of snakeheads in Laguna de Bay have yet to pinpoint the pathogen. Skin lesions in tilapias in several ponds and lakes in the country were found to be due to bacteria.
Conference paperRM Coloso - In MR Catacutan, RM Coloso & BO Acosta (Eds.), Development and Use of Alternative Dietary Ingredients or Fish Meal Substitutes in Aquaculture Feed Formulation … Ingredients or Fish Meal Substitutes in Aquaculture Feed Formulation, 9-11 December 2014, Nay Pyi Taw, Myanmar, 2015 - Aquaculture Department, Southeast Asian Fisheries Development CenterFish production from aquaculture in Asia has steadily increased during the past decade. In 2012, Asia s share in the total world aquaculture production was about 89% with 60 M metric tons valued at US$ 120 B. ASEAN Member States such as Viet Nam, Indonesia, Thailand, Myanmar, and the Philippines are among the top producers in Asia contributing 9 M metric tons of production from aquaculture valued at US$ 19 B (FAO, 2014). To sustain the production and profitability of aquaculture operations, reducing costs is needed mainly through feeds and feeding which represent up to 60% of operational costs. Reductions in feeding costs can be realized through optimizing nutrient levels of diets, feeding strategies, and by using plant protein sources as fish meal substitutes. As more intensive methods for production of the top five commodities (carps, tilapia, milkfish, catfish, and Pangasius sp.) become popular in ASEAN Member States, practical feeds need to be formulated using plant protein sources that are locally available. Plant protein sources such as soy proteins and corn gluten have been used as partial or total replacements for fish meal quite extensively in aquafeed for the top aquaculture commodities because of their high protein content (40-60%) and good digestibility. Other alternative dietary protein sources with emphasis on oilseed meals, peas and other leguminous seed meals, leaf meals from terrestrial plants, aquatic plants, plant protein concentrates, single cell proteins, cereal by products, fermentation and other products have been or are currently being evaluated as fish meal substitutes for their nutritive values, inclusion levels, constraints in processing mainly to reduce the effects of anti-nutritional factors as well as economic value. The proper use of these ingredients would promote good fish growth, survival, production, and boost the income of small scale farmers. Testing of aquaculture feeds containing these local ingredients will help the regional as well as worldwide research and development efforts and ultimately benefit the local small scale fish farmers and other stakeholders.
Conference paperIG Borlongan, CL Marte & J Nocillado - In CL Marte, GF Quinitio & AC Emata (Eds.), Proceedings of the Seminar-Workshop on Breeding and Seed Production of Cultured Finfishes in the Philippines, Tigbauan, Iloilo, Philippines, 4-5 May 1993, 1996 - SEAFDEC Aquaculture DepartmentA preliminary feeding experiment was conducted to determine growth and survival of milkfish larvae reared on various feeding regimes involving the use of artificial diets. Two larval diets (Feed A and Feed B) containing 45% protein and 10% lipid were fed either alone or in combination with Brachionus from day 8 to day 21. The feed in the control treatment were Brachionus (10 ind/ml) from day 8 to day 14 and Artemia (2-3 ind/ml) from day 15 to day 21. Larvae in all treatments were fed Brachionus (10 ind/ml) from day 2 to day 7. No significant differences were observed in survival rates, total length, wet weight and dry weight among fish fed combination of Brachionus and Feed B and the control feed (Brachionus and Artemia). These promising results indicate the possibility of using Feed B as partial replacement or supplement to live food. However, lowest survival rates, total length, and weight were obtained in fish fed either Feed A or Feed B alone, indicating that the test artificial diets given solely to milkfish larvae starting from day 8 can not support good growth and survival. Further studies on the development of improved artificial diets for larval milkfish need to be done.