SEAFDEC/AQDINSTITUTIONAL REPOSITORY
    • English
    • 日本語
    • ไทย
    • Bahasa Indonesia
  • 日本語 
    • English
    • 日本語
    • ไทย
    • Bahasa Indonesia
  • ログイン
アイテム表示 
  •   ホーム
  • 03 SEAFDEC/AQD External Publications
  • Journal Articles, Conference Papers and Book Chapters by SEAFDEC Staff
  • Conference Proceedings
  • アイテム表示
  •   ホーム
  • 03 SEAFDEC/AQD External Publications
  • Journal Articles, Conference Papers and Book Chapters by SEAFDEC Staff
  • Conference Proceedings
  • アイテム表示
JavaScript is disabled for your browser. Some features of this site may not work without it.

Electrophoretic studies on induced gynogenetic diploids and triploids in tilapia (Oreochromis niloticus and O. aureus)

  • Global styles
  • MLA
  • Vancouver
  • Elsevier - Harvard
  • APA
  • Help
Thumbnail
閲覧/開く
Request a copy
日付
1988
著者
Romana, Maria Rowena R. ORCID
Page views
2,422
主題
tilapia culture ASFA
breeding stock ASFA
genetics ASFA
enzymes ASFA
electrophoresis ASFA
polyploids ASFA
Philippines AGROVOC
Nile tilapia AGROVOC
tilapia AGROVOC
Oreochromis niloticus AGROVOC
Oreochromis aureus AGROVOC
Taxonomic term
Oreochromis niloticus GBIF
Oreochromis aureus GBIF
Metadata
アイテムの詳細レコードを表示する

Share 
 
抄録
Preliminary electrophoretic screening of laboratory maintained tilapia stocks revealed enzyme polymorphism at 3 co-dominantly inherited loci--adenosine deaminase (Ada), aminopeptidase (Ap) and malic enzyme (Me-2). Oreochromis niloticus and O. aureus broodstock used in artificial gynogenesis and polyploidy experiments were genetically tagged with these biochemical markers. Results of manipulations to induce diploid gynogenetic and triploid broods from heterozygous females were determined by genetic analysis.

Segregation ratios in the control broods confirmed Mendelian inheritance at the Me-2 and Ada loci. Genetic analysis of enzyme polymorphism in gynogenetic broods, produced from ova fertilized with genetically inert sperm and heat-shocked 5 minutes after fertilization indicated diploidy restoration by second polar body retention. Diploidisation of gynogenomes by suppression of first cleavage of mitosis in the zygote, attempted by heat shocking eggs 20-45 minutes after fertilization with UV-treated sperm, proved effective in one brood (heat shock at 45 min. after fertilization) in which a high incidence (~100% ) of individuals homozygous for Me-2, was observed. Finally, electrophoretic analysis of triploids revealed banding patterns different from those observed in normal and gynogenetic diploids. Such as banding phenotypes, peculiar only to triploids, denoted success in triploidy induction which was achived here with the fusion of the pronucleus and the maternal genome made double by suppresion of meiosis II.
URI
http://hdl.handle.net/10862/308
Type
Conference paper
Collections
  • Conference Proceedings [289]

Related items

Showing items related by title, author, creator and subject.

  • Thumbnail

    Tilapia farming in cages and ponds 

    Eguia, Ruel V.; Romana-Eguia, Ma. Rowena R. ORCID (Aquaculture Department, Southeast Asian Fisheries Development Center, 2004)
    This 40-page manual describes the farming practices for tilapia in cages, pens, ponds, and tanks. Also details selection of quality seedstock, maintenance of stock (feeding, water management), and harvesting. A list of ...Series: Aquaculture extension manual; No. 36
  • Thumbnail

    [The Philippines recommends for tilapia:] Introduction 

    Yap, Wilfredo G.; Eguia, Maria Rowena R.; Abella, Tereso A.; The Tilapia Technical Committee 2017 (DOST-PCAARRD, 2018)
  • Thumbnail

    Producing 100% male tilapia 

    Castaños, Milagros T.; Southeast Asian Fisheries Development Center, Aquaculture Department (Aquaculture Department, Southeast Asian Fisheries Development Center, 1993)

© SEAFDEC/AQD  2022
連絡先 | ご意見をお寄せ下さい
 

 

ブラウズ

リポジトリ全体コミュニティ/コレクション公開日著者タイトル主題このコレクション公開日著者タイトル主題

登録利用者

ログイン登録

統計

View Usage Statistics

© SEAFDEC/AQD  2022
連絡先 | ご意見をお寄せ下さい
 

 

Export citations

Export the current results of the search query as a citation list. Select one of the available citation styles, or add a new one using the "Citations format" option present in the "My account" section.

The list of citations that can be exported is limited to items.

Export citations

Export the current item as a citation. Select one of the available citation styles, or add a new one using the "Citations format" option present in the "My account" section.

Export Citations

DOCUMENT REQUEST NOT AVAILABLE

This publication is still available (in PRINT) and for sale at AQD bookstore. The library is currently restricted to send PDF of publications that are still for sale.

You may contact bookstore@seafdec.org.ph or visit AQD bookstore for orders.

FILE UNDER EMBARGO

This file associated with this publication is currently under embargo. This will be available for download after the embargo date.